LOCUS BBa_K415300 2494 bp DNA linear 31-Oct-2024 DEFINITION Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt ACCESSION BBa_K415300 VERSION BBa_K415300.1 FEATURES Location/Qualifiers misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 promoter 38..43 /label=-10 CDS 56..1183 /label=lacI-LVA misc_feature 56..1183 /label=BBa_C0012 misc_feature 1145..1183 /label=LVA misc_feature 1184..1208 /label=barcode misc_feature 1217..1416 /label=BBa_R0010 CDS 1217..1304 /label=end of LacI coding region (inactive) misc_feature 1305..1342 /label=CAP binding site promoter 1353..1358 /label=-35 promoter 1377..1382 /label=-10 misc_feature 1382..1416 /label=LacI binding site misc_feature 1389..1389 /label=start CDS 1426..2133 /label=cI lambda misc_feature 2134..2166 /label=LVA misc_feature 1423..2172 /label=BBa_C0051 misc_feature 2173..2197 /label=barcode ORIGIN 1 gtcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 61 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg 121 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt 181 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat 241 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca 301 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca 361 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg 421 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat 481 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga 541 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg 601 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat 661 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca 721 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc 781 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg 841 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca 901 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca 961 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc 1021 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt 1081 tcatgacgtc catccggccg tcctttgcat acaccatagg tgtggtttaa tttgatgccc 1141 tttttcaggg ctggaatgtg taagagcggg gttatttatg ctgttgtttt tttgttactc 1201 gggaagggct ttacctcttc cgcataaacg cttccatcag cgtttatagt taaaaaaatc 1261 tttcggaact ggttttgcgc ttaccccaac caacagggga tttgctgctt tccattgagc 1321 ctgtttctct gcgcgacgtt cgcggcggcg tgtttgtgca tccatctgga ttctcctgtc 1381 agttagcttt ggtggtgtgt ggcagttgta gtcctgaacg aaaacccccc gcgattggca 1441 cattggcagc taatccggaa tcgcacttac ggccaatgct tcgtttcgta tcacacaccc 1501 caaagccttc tgctttgaat gctgcccttc ttcagggctt aatttttaag agcgtcacct 1561 tcatggtggt cagtgcgtcc tgctgatgtg ctcattataa ccgccagtgg tatttatgtc 1621 aacaccgcca gagataattt atcaccgcag atggttatct gtgcatgcat ttacgttgac 1681 accatcgaat ggctgaaatg agctgttgac aattaatcat ccggctcgta taatgtgtgg 1741 aattgtgagc ggataacaat ttcacacagg aaaccggtta tgagcacaaa aaagaaacca 1801 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa 1861 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc 1921 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca 1981 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag 2041 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt 2101 tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg 2161 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa 2221 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt 2281 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt 2341 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta 2401 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc 2461 gctagtcagt ggcctgaaga gacgtttggc tgat //