BBa_K415300
1
Toggle
Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt
2010-10-22T11:00:00Z
2015-05-08T01:12:27Z
Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.
Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.The Collins UV Toggle (2000) biobricked. It is a bistable toggle (on or off state) and switching is induced by UV exposure or IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
false
false
_525_
0
6736
9
It's complicated
false
We used PCR to biobrick this sequence from its original form in an Amp plasmid with SC101 origin. We had to transfer into pSB1C3 to submit it to the registry.
false
Leanna Morinishi, Grant Robinson, Andrew Yang, Paul Muir, Shirley Galbiati, Shawn Pan, Jason Stevens, Lauren McGough, Deepak Mishra, Ron Weiss
annotation2093352
1
lacI-LVA
range2093352
1
56
1183
annotation2093358
1
CAP binding site
range2093358
1
1305
1342
annotation2093354
1
LVA
range2093354
1
1145
1183
annotation2093362
1
start
range2093362
1
1389
1389
annotation2093363
1
cI lambda
range2093363
1
1426
2133
annotation2093366
1
barcode
range2093366
1
2173
2197
annotation2093350
1
OR1
range2093350
1
25
41
annotation2093364
1
LVA
range2093364
1
2134
2166
annotation2093348
1
BBa_R0051
range2093348
1
1
49
annotation2093357
1
end of LacI coding region (inactive)
range2093357
1
1217
1304
annotation2093347
1
OR2
range2093347
1
1
17
annotation2093349
1
-35
range2093349
1
15
20
annotation2093359
1
-35
range2093359
1
1353
1358
annotation2093355
1
barcode
range2093355
1
1184
1208
annotation2093356
1
BBa_R0010
range2093356
1
1217
1416
annotation2093351
1
-10
range2093351
1
38
43
annotation2093360
1
-10
range2093360
1
1377
1382
annotation2093361
1
LacI binding site
range2093361
1
1382
1416
annotation2093365
1
BBa_C0051
range2093365
1
1423
2172
annotation2093353
1
BBa_C0012
range2093353
1
56
1183
BBa_K415300_sequence
1
gtcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcatgacgtccatccggccgtcctttgcatacaccataggtgtggtttaatttgatgccctttttcagggctggaatgtgtaagagcggggttatttatgctgttgtttttttgttactcgggaagggctttacctcttccgcataaacgcttccatcagcgtttatagttaaaaaaatctttcggaactggttttgcgcttaccccaaccaacaggggatttgctgctttccattgagcctgtttctctgcgcgacgttcgcggcggcgtgtttgtgcatccatctggattctcctgtcagttagctttggtggtgtgtggcagttgtagtcctgaacgaaaaccccccgcgattggcacattggcagctaatccggaatcgcacttacggccaatgcttcgtttcgtatcacacaccccaaagccttctgctttgaatgctgcccttcttcagggcttaatttttaagagcgtcaccttcatggtggtcagtgcgtcctgctgatgtgctcattataaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtgcatgcatttacgttgacaccatcgaatggctgaaatgagctgttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaaccggttatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z