BBa_K415301 1 LPToggle Low Power Toggle - Ptrc:cI:tt:Plambda:LacI:tt 2010-10-24T11:00:00Z 2015-05-08T01:12:27Z From the [[http://2010.igem.org/Team:MIT|MIT 2010 iGEM Team]]. This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS. [[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. This plasmid has been modified so that it now contains cI that is hypersensitive to cleavage by RecA = Low Power Toggle.]] This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.<br><br>This part improves upon its predecessor in that it requires less UV power to switch states, thus killing fewer cells (see E.Coli death curve).<br><br> [[Image:Deathcurve.png|frame|150px|right|E.Coli population density as a function of UV exposure. Measured by drip assay.]] The following data was taken from cells co-transformed with K415301 (with and [[Part:BBa_K415023]]. {|style="border-width: 1px; width: 200px; border-style: solid; border-color: #000; width: 700px; text-align:center;" !colspan="6"| == UV Power Modulation == |- |colspan="2"|'''Photos''' (in J/m^2, ignore W) |colspan="4"| |- |colspan="2"|'''FACS''' (in J/m^2) |colspan="1"|[[Image:]] |colspan="1"|[[Image:]] |colspan="1"|[[Image:]] |colspan="1"|[[Image:]] |- |colspan="2"| |colspan="1"|0 |colspan="1"|5 |colspan="1"|20 |colspan="1"|40 |} false false _525_ 0 6736 9 It's complicated false None. false iGEM 2010 MIT BBa_K415301_sequence 1 gtcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcatgacgtccatccggccgtcctttgcatacaccataggtgtggtttaatttgatgccctttttcagggctggaatgtgtaagagcggggttatttatgctgttgtttttttgttactcgggaagggctttacctcttccgcataaacgcttccatcagcgtttatagttaaaaaaatctttcggaactggttttgcgcttaccccaaccaacaggggatttgctgctttccattgagcctgtttctctgcgcgacgttcgcggcggcgtgtttgtgcatccatctggattctcctgtcagttagctttggtggtgtgtggcagttgtagtcctgaacgaaaaccccccgcgattggcacattggcagctaatccggaatcgcacttacggccaatgcttcgtttcgtatcacacaccccaaagccttctgctttgaatgctgcccttcttcagggcttaatttttaagagcgtcaccttcatggtggtcagtgcgtcctgctgatgtgctcattataaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtgcatgcatttacgttgacaccatcgaatggctgaaatgagctgttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaaccggttatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttggctgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z