BBa_K415500 1 BMP2 BMP2 L1L2 MammoBlock Entry Vector 2010-10-23T11:00:00Z 2015-05-08T01:12:27Z NCBI Link: http://www.ncbi.nlm.nih.gov/gene/650 cDNA for human homolog obtained from OpenBiosystems. BBa_K415500 is an L1L2 Entry Vector for MammoBlock recombination cloning, containing the gene BMP2 (see below) BMP2 (Bone Morphogenetic Protein 2) is a well estabished pro-osteogenic morphogen shown to induce osteogenesis in a variety of cell types in literature. Upon binding BMP2, the BMPRI receptor activates a downstream signaling cascade involving Smad proteins, which results in activation of the Runt-related transcription factor 2 (RUNX2). RUNX2 is the main transcription factor controlling osteoblast differentiation; transfection of RUNX2 induced osteoblastic differentiation of fibroblasts, which do not normally differentiate into osteoblasts. false false _525_ 0 6768 9 It's complicated false The plasmid was assembled by using primers with attB recombination sites to PCR a BMP2 insert with attB sites on either side. This construct was inserted into a P1P2 donor vector using the Gateway recombination system to yield an L1L2 MammoBlock entry vector. false Laura Deming, Joy Jiao, Adrian Slusarczyk annotation2095276 1 Stop Codon range2095276 1 1215 1217 annotation2095275 1 Start Codon range2095275 1 24 26 BBa_K415500_sequence 1 ggatcccggtaccggtcgccaccatggtggccgggacccgctgtcttctagcgttgctgcttccccaggtcctcctgggcggcgcggctggcctcgttccggagctgggccgcaggaagttcgcggcggcgtcgtcgggccgcccctcatcccagccctctgacgaggtcctgagcgagttcgagttgcggctgctcagcatgttcggcctgaaacagagacccacccccagcagggacgccgtggtgcccccctacatgctagacctgtatcgcaggcactcaggtcagccgggctcacccgccccagaccaccggttggagagggcagccagccgagccaacactgtgcgcagcttccaccatgaagaatctttggaagaactaccagaaacgagtgggaaaacaacccggagattcttctttaatttaagttctatccccacggaggagtttatcacctcagcagagcttcaggttttccgagaacagatgcaagatgctttaggaaacaatagcagtttccatcaccgaattaatatttatgaaatcataaaacctgcaacagccaactcgaaattccccgtgaccagacttttggacaccaggttggtgaatcagaatgcaagcaggtgggaaagttttgatgtcacccccgctgtgatgcggtggactgcacagggacacgccaaccatggattcgtggtggaagtggcccacttggaggagaaacaaggtgtctccaagagacatgttaggataagcaggtctttgcaccaagatgaacacagctggtcacagataaggccattgctagtaacttttggccatgatggaaaagggcatcctctccacaaaagagaaaaacgtcaagccaaacacaaacagcggaaacgccttaagtccagctgtaagagacaccctttgtacgtggacttcagtgacgtggggtggaatgactggattgtggctcccccggggtatcacgccttttactgccacggagaatgcccttttcctctggctgatcatctgaactccactaatcatgccattgttcagacgttggtcaactctgttaactctaagattcctaaggcatgctgtgtcccgacagaactcagtgctatctcgatgctgtaccttgacgagaatgaaaaggttgtattaaagaactatcaggacatggttgtggagggttgtgggtgtcgctgataagaattctacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z