LOCUS       BBa_K415504             2187 bp    DNA     linear       28-Dec-2024
DEFINITION  Rtta3_2a_Hygro L1L2 MammoBlock Entry Vector
ACCESSION   BBa_K415504
VERSION     BBa_K415504.1  
FEATURES             Location/Qualifiers
     CDS             363..1068
                     /label=rtTA3
     misc_feature    1069..1128
                     /label=2A
     CDS             1129..2169
                     /label=Hygro
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac tactagaggg atccccgggt accggtcgcc
      361 accatgtcta ggctggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
      421 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
      481 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
      541 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
      601 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
      661 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
      721 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
      781 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
      841 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
      901 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
      961 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     1021 ctcccagccg atgcccttga cgattttgac cttgacatgc tccccgggga attggagggc
     1081 cgcggcagcc tgctgacctg cggcgacgtg gaggaaaacc ccggccccat gaaaaagcct
     1141 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     1201 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     1261 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     1321 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     1381 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     1441 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     1501 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     1561 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctcctggcaa
     1621 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     1681 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     1741 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     1801 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     1861 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     1921 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     1981 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     2041 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     2101 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag
     2161 gctaactgag cggccgcgaa ttctacc
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