BBa_K416003
1
BBa_K416003
Yeast Secretion Tag
2010-06-22T11:00:00Z
2015-05-08T01:12:27Z
This part was sent to use from Dr. Sheldon Park of the University of Buffalo.
This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS.
false
false
_527_
0
7430
9
It's complicated
true
There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
false
John Phair
BBa_K416003_sequence
1
atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z