BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K105009 1 ECFP ECFP, yeast optimized for fusion proteins 2008-10-11T11:00:00Z 2015-05-08T01:08:52Z We changed the format of the ECFP coding part of [[Part:BBa_E2020|BBa_E2020]] into Phillips/Silver standard. Our BioBrick is cyan fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of BioBrick part for fusion proteins (see design details). false false _253_ 0 3313 9 It's complicated false To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006). false Katja Karstens BBa_K416008 1 BBa_K416008 Aga2 fused to eCFP 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z This part comes from a mixture of biobricks that were received in the 2010 initial distribution and from parts that NYU iGEM 2010 made ourselves. When working correctly this reporter construct transcribes Aga2-eCFP from the Gal promoter. If the yeast strain is also expressing Aga1, then this fusion should be anchored onto the plasma membrane, allowing you to visualize eCFP as a halo around the yeast cell. false false _527_ 0 6443 9 Not in stock true We considered fusing this reporter to a variety of promoters, but in the end chose pGAL because it gives a very high level of transcription and we could turn the reporter on and off false Russell Durrett component2110286 1 BBa_K416001 component2110288 1 BBa_K106018 component2110283 1 BBa_K416005 component2110285 1 BBa_K416000 component2110287 1 BBa_K105009 annotation2110285 1 BBa_K416000 range2110285 1 582 842 annotation2110288 1 BBa_K106018 range2110288 1 1656 1835 annotation2110283 1 BBa_K416005 range2110283 1 1 575 annotation2110286 1 BBa_K416001 range2110286 1 851 895 annotation2110287 1 BBa_K105009 range2110287 1 904 1647 BBa_K416005 1 BBa_K416005 pGAL::RBS 2010-10-14T11:00:00Z 2015-05-08T01:12:27Z This part comes from the yeast Saccharomyces cerevisea and should be used therein. This composite part is the Gal promoter fused to a Ribosome Binding Sequence (or Kozak sequence). It should be used in yeast when the part immediately downstream should be translated. For more information see the pGAL part info - part ID: J63006 false false _527_ 0 6443 9 Not in stock false We designed this part in order to be able to incorporate constructs that we would like to see translated without having to insert an RBS each time. We believe that this promoter sequence fused to the RBS will be useful for future researchers working in Saccharomyces. false Russell Durrett component2086422 1 BBa_K165002 component2086421 1 BBa_J63006 annotation2086421 1 BBa_J63006 range2086421 1 1 549 annotation2086422 1 BBa_K165002 range2086422 1 558 575 BBa_K416000 1 BBa_K416000 Aga2 CDS Responsible for Yeast Surface Display 2010-05-27T11:00:00Z 2015-05-08T01:12:27Z This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT. The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell. The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions. false false _527_ 0 6443 9 Not in stock true We removed the STOP codon to allow for N- and C-terminal fusions, which have both been proven to occur efficiently. false Russell Durrett annotation2069499 1 Aga2 CDS range2069499 1 1 261 BBa_K106018 1 BBa_K106018 Adh1 terminator 2008-10-23T11:00:00Z 2015-05-08T01:08:58Z Cerevisiae. Adh1 terminator for cerevisiae. false false _226_ 0 2659 9 Not in stock false None. false Andrew Horwitz BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K416001 1 BBa_K416001 (Gly4Ser)3 Flexible Peptide Linker 2010-05-28T11:00:00Z 2015-05-08T01:12:27Z The DNA came from K.D. Wittrup's lab at MIT, on the pCTCON plasmid used for yeast scFv surface display. This is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. The linker is to be fused in frame in between two protein domains, separating the two domains so that they each retain original functions yet will be physically connected. false false _527_ 0 6459 9 It's complicated false This sequence is meant to be translated between two different protein domains, so no start or stop codon is present. false Harris Kaplan BBa_K105009_sequence 1 gcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgca BBa_K165002_sequence 1 cccgccgccaccatggag BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_K106018_sequence 1 gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacc BBa_K416005_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagagcccgccgccaccatggag BBa_K416000_sequence 1 atgcagttacttcgctgtttttcaatattttctgttattgcttcagttttagcacaggaactgacaactatatgcgagcaaatcccctcaccaactttagaatcgacgccgtactctttgtcaacgactactattttggccaacgggaaggcaatgcaaggagtttttgaatattacaaatcagtaacgtttgtcagtaattgcggttctcacccctcaacaactagcaaaggcagccccataaacacacagtatgttttt BBa_K416001_sequence 1 ggtggaggaggctctggtggaggcggtagcggaggcggagggtcg BBa_K416008_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagagcccgccgccaccatggagtactagatgcagttacttcgctgtttttcaatattttctgttattgcttcagttttagcacaggaactgacaactatatgcgagcaaatcccctcaccaactttagaatcgacgccgtactctttgtcaacgactactattttggccaacgggaaggcaatgcaaggagtttttgaatattacaaatcagtaacgtttgtcagtaattgcggttctcacccctcaacaactagcaaaggcagccccataaacacacagtatgttttttactagagggtggaggaggctctggtggaggcggtagcggaggcggagggtcgtactagaggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattctggttgaattggatggcgatgtcaatggccataagttttcggttagtggtgaaggagagggcgacgctacctatgggaagttaactttaaagttcatttgtactaccggtaagttaccagttccttggcctactttggtcacaacccttacatggggggtgcagtgctttgccagatatccggatcacatgaaacaacacgattttttcaaatccgctatgcctgaaggatatgtacaagaaagaaccatatttttcaaggatgacggcaactacaaaactagagccgaagttaaattcgaaggtgacacattggtaaatcgaattgagctcaaaggaatagattttaaggaagatggtaacatccttggtcataagttagagtataatgcaatttctgataacgtctacataactgcggataaacagaaaaatggtattaaagccaattttaaaattaggcataacatcgaagatgggagtgttcaacttgcagaccactaccaacaaaatacacccataggagacggtcccgtactgttgccagataaccattatctgtctacacaatctaaattaagcaaagatccaaatgaaaagcgtgaccacatggtgttgctagagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcatactagaggcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z