LOCUS BBa_K417006 3808 bp DNA linear 22-Dec-2024 DEFINITION LacI ethylene generator ACCESSION BBa_K417006 VERSION BBa_K417006.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1381 /label=SAMsynth misc_feature 227..227 /label=start misc_feature 1379..1379 /label=stop CDS 227..1381 /label=BBa_K417000 misc_feature 209..1381 /label=BBa_K417003 misc_feature 1394..1397 /label=conserved RBS 1390..1401 /label=BBa_B0034 misc_feature 1408..1410 /label=Start CDS 1408..2697 /label=ACC Synth misc_feature 2695..2697 /label=Stop CDS 1408..2697 /label=BBa_K417001 misc_feature 1390..2697 /label=BBa_K417004 misc_feature 2710..2713 /label=conserved RBS 2706..2717 /label=BBa_B0034 misc_feature 2724..2726 /label=Start CDS 2724..3671 /label=ACC Oxidase misc_feature 3669..3671 /label=Stop CDS 2724..3671 /label=BBa_K417002 misc_feature 2706..3671 /label=BBa_K417005 stem_loop 3691..3734 /label=stem_loop misc_feature 3680..3759 /label=BBa_B0010 terminator 3680..3759 /label=BBa_B0010 stem_loop 3775..3794 /label=T7 TE misc_feature 3801..3801 /label=stop polyA_site 3795..3808 /label=polya misc_feature 3768..3808 /label=BBa_B0012 terminator 3768..3808 /label=BBa_B0012 terminator 3680..3808 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caaaacacct 241 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatttctga 301 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta 361 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat 421 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt 481 tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca 541 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg 601 ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct 661 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc 721 gaaaagccag gtgacttttc agtatgacga cggcaaaatc gttggtatcg atgctgtcgt 781 gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga 841 agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat 901 caacccgacc ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggtcg 961 taaaattatc gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg 1021 taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa 1081 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt 1141 ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca 1201 actgaccctg ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct 1261 ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca 1321 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta 1381 atactagaga aagaggagaa atactagatg ctgtctcgta atgccacctt caatagccat 1441 ggtcaggact cttcgtattt tctgggttgg caggagtatg aaaaaaaccc ttatcacgag 1501 gtccataaca ccaacggcat catccaaatg ggcctggctg agaatcaact gtgctttgac 1561 ctgctggaat cttggctggc caaaaatccg gaagcagctg ccttcaaaaa aaacggcgag 1621 agcattttcg ctgaactggc cctgtttcaa gactatcatg ggctgcctga gttcaaaaaa 1681 gccatggtgg actttatggc cgaaattcgt gggaacaaag tgacctttga cccgaatcat 1741 ctggttctga ctgctggtgc tacaagtgct aacgaaacgt tcatcttttg tctggcagat 1801 cctggtgaag ctgtactgat cccgacaccg tattatccag gcttcgatcg tgacctgaaa 1861 tggcgtacag gtgtggaaat cgtaccgatc cattgtacca gctctaatgg tttccagatc 1921 acggaaacgg cactggaaga agcatatcag gaggccgaga aacgtaatct gcgcgtgaaa 1981 ggtgtactgg tgaccaatcc gtctaatcct ctgggtacta ctatgacccg taacgaactg 2041 tatctgctgc tgtcctttgt ggaggataaa ggcattcacc tgattagcga cgagatctat 2101 tccgggaccg cttttagtag cccgagcttt attagtgtga tggaggtgct gaaagaccgt 2161 aattgtgatg agaacagtga ggtctggcaa cgtgttcatg tggtgtatag cctgtccaaa 2221 gacctgggac tgcctggttt tcgtgttggc gccatctata gcaacgatga catggttgtg 2281 gctgccgcta caaaaatgag cagcttcggt ctggtttctt ctcaaaccca acatctgctg 2341 tcagcaatgc tgtccgataa aaaactgacc aaaaactata tcgccgagaa tcataaacgc 2401 ctgaaacaac gccaaaaaaa actggtgtct ggcctgcaaa aatctggtat cagttgcctg 2461 aacggtaatg ctggcctgtt ttgctgggtc gacatgcgtc acctgctgcg ttctaacaca 2521 ttcgaggccg aaatggaact gtggaaaaaa atcgtgtatg aggttcatct gaacattagc 2581 ccgggttctt catgccactg tactgaaccg ggttggtttc gtgtgtgttt cgccaatctg 2641 cctgagcgta ctctggatct ggctatgcaa cgtctgaaag ccttcgtggg cgaataatac 2701 tagagaaaga ggagaaatac tagatggaaa acttcccgat catcaacctg gagaaactga 2761 atggtgacga acgtgccaat acaatggaaa tgattaaaga cgcctgtgag aactggggct 2821 tttttgaact ggtgaaccac ggtatccctc atgaagtgat ggacacggtc gagaaaatga 2881 ccaaaggcca ctataaaaaa tgtatggagc agcgctttaa agagctggtt gccagcaaag 2941 gtctggaggc tgttcaggca gaagtgaccg atctggattg ggagagcacc ttctttctgc 3001 gtcatctgcc gaccagtaac attagtcagg tgccggacct ggatgaagaa tatcgtgagg 3061 tcatgcgcga tttcgccaaa cgcctggaaa aactggccga agaactgctg gatctgctgt 3121 gtgaaaatct ggggctggaa aaaggttatc tgaaaaacgc cttctatggc tctaaaggac 3181 cgaattttgg gacgaaagtc agcaattatc cgccttgtcc gaaaccggat ctgatcaaag 3241 gactgcgtgc ccacaccgat gccggtggta ttatcctgct gtttcaagac gacaaagtgt 3301 caggcctgca actgctgaaa gacgaacagt ggatcgatgt tcctcctatg cgccactcta 3361 ttgtggtgaa tctgggtgac caactggagg ttattacgaa cggaaaatat aaaagtgtgc 3421 tgcaccgcgt tattgctcag acagatggca cccgtatgag tctggcatca ttctataatc 3481 cgggctctga tgctgtaatc tatccggcca aaacgctggt agaaaaagag gccgaggagt 3541 ctacacaggt ttatccgaaa tttgtgttcg acgactatat gaaactgtat gccgggctga 3601 aattccaagc aaaagaacct cgcttcgaag ccatgaaagc aatggagagc gatccgattg 3661 ctagtgccta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3721 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3781 ccttcgggtg ggcctttctg cgtttata //