BBa_K417004 1 BBa_K417004 RBS + ACC Synthase 2010-10-25T11:00:00Z 2015-05-08T01:12:28Z Apple RBS + ACC Synthase false false _537_ 0 6513 9 Not in stock false Nil false Benjamin Porebski component2101564 1 BBa_K417001 component2101560 1 BBa_B0034 annotation2101564 1 BBa_K417001 range2101564 1 19 1308 annotation2101560 1 BBa_B0034 range2101560 1 1 12 BBa_K417003 1 BBa_K417003 RBS + SAM Synthetase 2010-10-25T11:00:00Z 2015-05-08T01:12:28Z E. coli genomic DNA RBS + SAM Synthetase false false _537_ 0 6513 9 Not in stock false Nil false Benjamin Porebski component2101553 1 BBa_K417000 component2101550 1 BBa_B0034 annotation2101550 1 BBa_B0034 range2101550 1 1 12 annotation2101553 1 BBa_K417000 range2101553 1 19 1173 BBa_K417000 1 SAMsynth S-adenosyl methionine (SAM) synthetase 2010-10-13T11:00:00Z 2015-05-08T01:12:27Z Part was cloned from genomic DNA of Escherichia coli strain BL21(DE3) using PCR. This is the protein coding sequence of S-adenosyl methionine synthetase, which catalyses the formation of S-adenosyl methionine synthetase (AdoMet) from ATP and methionine (ATP + L-methionine + H(2)O <=> phosphate + diphosphate + S-adenosyl-L-methionine). Other names for this enzyme are AdoMet synthetase, methionine adenosyltransferase. It is the product of the metK gene in E. coli. false false _537_ 0 6167 9 It's complicated true The genomic sequence corresponding to this part does not contain any illegal restriction sites with respect to RFC10, so no mutagenesis of the native sequence was required. false Ben Porebski, Andrew Perry annotation2085975 1 stop range2085975 1 1153 1153 annotation2085973 1 SAMsynth range2085973 1 1 1155 annotation2085974 1 start range2085974 1 1 1 BBa_K417005 1 BBa_K417005 RBS + ACC Oxidase 2010-10-25T11:00:00Z 2015-05-08T01:12:28Z Tomato RBS + ACC Oxidase false false _537_ 0 6513 9 Not in stock false Nil false Benjamin Porebski component2101581 1 BBa_B0034 component2101585 1 BBa_K417002 annotation2101581 1 BBa_B0034 range2101581 1 1 12 annotation2101585 1 BBa_K417002 range2101585 1 19 966 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K417002 1 ACCoxidase 1-aminocyclopropane-1-carboxylate (ACC) oxidase 2010-10-19T11:00:00Z 2015-05-08T01:12:28Z Solanum lycopersicum (Tomato) (Lycopersicon esculentum) codon optimized for <i>E. coli</i>. This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, which catalyses the formation of ethylene from 1-aminocyclopropane-1-carboxylate under the presence of ascorbate and O2. (1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O) This enzyme is commonly referred to as ACC oxidase or ethylene forming enzyme (EFE). false false _537_ 0 6513 9 Not in stock false Codon optimization for <i>E. coli</i> and removal of biobrick restriction sites. false Ben Porebski, Andrew Perry annotation2090378 1 Start range2090378 1 1 3 annotation2090379 1 ACC Oxidase range2090379 1 1 948 annotation2090380 1 Stop range2090380 1 946 948 BBa_K417001 1 ACCsynth 1-aminocyclopropane-1-carboxylate (ACC) synthase 2010-10-19T11:00:00Z 2015-05-08T01:12:28Z Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet). (S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine). This enzyme is commonly referred to as ACC synthase. false false _537_ 0 6513 9 Not in stock false Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity. false Ben Porebski, Andrew Perry annotation2090377 1 Stop range2090377 1 1288 1290 annotation2090375 1 Start range2090375 1 1 3 annotation2090376 1 ACC Synth range2090376 1 1 1290 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K417006 1 BBa_K417006 LacI ethylene generator 2010-10-25T11:00:00Z 2015-05-08T01:12:28Z E. coli genomic DNA, Apple and Tomato LacI regulated ethylene generator that uses 3 enzymes, blah false false _537_ 0 6513 9 Not in stock false Nil false Benjamin Porebski component2101895 1 BBa_R0010 component2101928 1 BBa_B0015 component2101907 1 BBa_K417003 component2101914 1 BBa_K417004 component2101921 1 BBa_K417005 annotation2101914 1 BBa_K417004 range2101914 1 1390 2697 annotation2101895 1 BBa_R0010 range2101895 1 1 200 annotation2101928 1 BBa_B0015 range2101928 1 3680 3808 annotation2101921 1 BBa_K417005 range2101921 1 2706 3671 annotation2101907 1 BBa_K417003 range2101907 1 209 1381 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K417005_sequence 1 aaagaggagaaatactagatggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaa BBa_K417003_sequence 1 aaagaggagaaatactagatggcaaaacacctttttacgtccgagtccgtctctgaagggcatcctgacaaaattgctgaccaaatttctgatgccgttttagacgcgatcctcgaacaggatccgaaagcacgcgttgcttgcgaaacctacgtaaaaaccggcatggttttagttggcggcgaaatcaccaccagcgcctgggtagacatcgaagagatcacccgtaacaccgttcgcgaaattggctatgtgcattccgacatgggctttgacgctaactcctgtgcggttctgagcgctatcggcaaacagtctcctgacatcaaccagggcgttgaccgtgccgatccgctggaacagggcgcgggtgaccagggtctgatgtttggctacgcaactaatgaaaccgacgtgctgatgccagcacctatcacctatgcacaccgtctggtacagcgtcaggctgaagtgcgtaaaaacggcactctgccgtggctgcgcccggacgcgaaaagccaggtgacttttcagtatgacgacggcaaaatcgttggtatcgatgctgtcgtgctttccactcagcactctgaagagatcgaccagaaatcgctgcaagaagcggtaatggaagagatcatcaagccaattctgcccgctgaatggctgacttctgccaccaaattcttcatcaacccgaccggtcgtttcgttatcggtggcccaatgggtgactgcggtctgactggtcgtaaaattatcgttgatacctacggcggcatggcgcgtcacggtggcggtgcattctctggtaaagatccatcaaaagtggaccgttccgcagcctacgcagcacgttatgtcgcgaaaaacatcgttgctgctggcctggccgatcgttgtgaaattcaggtttcctacgcaatcggcgtggctgaaccgacctccatcatggtagaaactttcggtactgagaaagtgccttctgaacaactgaccctgctggtacgtgagttcttcgacctgcgcccatacggtctgattcagatgctggatctgctgcacccgatctacaaagaaaccgcagcatacggtcactttggtcgtgaacatttcccgtgggaaaaaaccgacaaagcgcagctgctgcgcgatgctgccggtctgaagtaa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K417002_sequence 1 atggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaa BBa_K417000_sequence 1 atggcaaaacacctttttacgtccgagtccgtctctgaagggcatcctgacaaaattgctgaccaaatttctgatgccgttttagacgcgatcctcgaacaggatccgaaagcacgcgttgcttgcgaaacctacgtaaaaaccggcatggttttagttggcggcgaaatcaccaccagcgcctgggtagacatcgaagagatcacccgtaacaccgttcgcgaaattggctatgtgcattccgacatgggctttgacgctaactcctgtgcggttctgagcgctatcggcaaacagtctcctgacatcaaccagggcgttgaccgtgccgatccgctggaacagggcgcgggtgaccagggtctgatgtttggctacgcaactaatgaaaccgacgtgctgatgccagcacctatcacctatgcacaccgtctggtacagcgtcaggctgaagtgcgtaaaaacggcactctgccgtggctgcgcccggacgcgaaaagccaggtgacttttcagtatgacgacggcaaaatcgttggtatcgatgctgtcgtgctttccactcagcactctgaagagatcgaccagaaatcgctgcaagaagcggtaatggaagagatcatcaagccaattctgcccgctgaatggctgacttctgccaccaaattcttcatcaacccgaccggtcgtttcgttatcggtggcccaatgggtgactgcggtctgactggtcgtaaaattatcgttgatacctacggcggcatggcgcgtcacggtggcggtgcattctctggtaaagatccatcaaaagtggaccgttccgcagcctacgcagcacgttatgtcgcgaaaaacatcgttgctgctggcctggccgatcgttgtgaaattcaggtttcctacgcaatcggcgtggctgaaccgacctccatcatggtagaaactttcggtactgagaaagtgccttctgaacaactgaccctgctggtacgtgagttcttcgacctgcgcccatacggtctgattcagatgctggatctgctgcacccgatctacaaagaaaccgcagcatacggtcactttggtcgtgaacatttcccgtgggaaaaaaccgacaaagcgcagctgctgcgcgatgctgccggtctgaagtaa BBa_K417006_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcaaaacacctttttacgtccgagtccgtctctgaagggcatcctgacaaaattgctgaccaaatttctgatgccgttttagacgcgatcctcgaacaggatccgaaagcacgcgttgcttgcgaaacctacgtaaaaaccggcatggttttagttggcggcgaaatcaccaccagcgcctgggtagacatcgaagagatcacccgtaacaccgttcgcgaaattggctatgtgcattccgacatgggctttgacgctaactcctgtgcggttctgagcgctatcggcaaacagtctcctgacatcaaccagggcgttgaccgtgccgatccgctggaacagggcgcgggtgaccagggtctgatgtttggctacgcaactaatgaaaccgacgtgctgatgccagcacctatcacctatgcacaccgtctggtacagcgtcaggctgaagtgcgtaaaaacggcactctgccgtggctgcgcccggacgcgaaaagccaggtgacttttcagtatgacgacggcaaaatcgttggtatcgatgctgtcgtgctttccactcagcactctgaagagatcgaccagaaatcgctgcaagaagcggtaatggaagagatcatcaagccaattctgcccgctgaatggctgacttctgccaccaaattcttcatcaacccgaccggtcgtttcgttatcggtggcccaatgggtgactgcggtctgactggtcgtaaaattatcgttgatacctacggcggcatggcgcgtcacggtggcggtgcattctctggtaaagatccatcaaaagtggaccgttccgcagcctacgcagcacgttatgtcgcgaaaaacatcgttgctgctggcctggccgatcgttgtgaaattcaggtttcctacgcaatcggcgtggctgaaccgacctccatcatggtagaaactttcggtactgagaaagtgccttctgaacaactgaccctgctggtacgtgagttcttcgacctgcgcccatacggtctgattcagatgctggatctgctgcacccgatctacaaagaaaccgcagcatacggtcactttggtcgtgaacatttcccgtgggaaaaaaccgacaaagcgcagctgctgcgcgatgctgccggtctgaagtaatactagagaaagaggagaaatactagatgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataatactagagaaagaggagaaatactagatggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K417001_sequence 1 atgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K417004_sequence 1 aaagaggagaaatactagatgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z