LOCUS       BBa_K417007             2627 bp    DNA     linear       16-Feb-2025
DEFINITION  Simple inducible (LacI) ethylene generator
ACCESSION   BBa_K417007
VERSION     BBa_K417007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start
     CDS             227..1516
                     /label=ACC Synth
     misc_feature    1514..1516
                     /label=Stop
     CDS             227..1516
                     /label=BBa_K417001
     misc_feature    1529..1532
                     /label=conserved
     RBS             1525..1536
                     /label=BBa_B0034
     misc_feature    1543..1545
                     /label=Start
     CDS             1543..2490
                     /label=ACC Oxidase
     misc_feature    2488..2490
                     /label=Stop
     CDS             1543..2490
                     /label=BBa_K417002
     stem_loop       2510..2553
                     /label=stem_loop
     misc_feature    2499..2578
                     /label=BBa_B0010
     terminator      2499..2578
                     /label=BBa_B0010
     stem_loop       2594..2613
                     /label=T7 TE
     misc_feature    2620..2620
                     /label=stop
     polyA_site      2614..2627
                     /label=polya
     misc_feature    2587..2627
                     /label=BBa_B0012
     terminator      2587..2627
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgtctcgtaa
      241 tgccaccttc aatagccatg gtcaggactc ttcgtatttt ctgggttggc aggagtatga
      301 aaaaaaccct tatcacgagg tccataacac caacggcatc atccaaatgg gcctggctga
      361 gaatcaactg tgctttgacc tgctggaatc ttggctggcc aaaaatccgg aagcagctgc
      421 cttcaaaaaa aacggcgaga gcattttcgc tgaactggcc ctgtttcaag actatcatgg
      481 gctgcctgag ttcaaaaaag ccatggtgga ctttatggcc gaaattcgtg ggaacaaagt
      541 gacctttgac ccgaatcatc tggttctgac tgctggtgct acaagtgcta acgaaacgtt
      601 catcttttgt ctggcagatc ctggtgaagc tgtactgatc ccgacaccgt attatccagg
      661 cttcgatcgt gacctgaaat ggcgtacagg tgtggaaatc gtaccgatcc attgtaccag
      721 ctctaatggt ttccagatca cggaaacggc actggaagaa gcatatcagg aggccgagaa
      781 acgtaatctg cgcgtgaaag gtgtactggt gaccaatccg tctaatcctc tgggtactac
      841 tatgacccgt aacgaactgt atctgctgct gtcctttgtg gaggataaag gcattcacct
      901 gattagcgac gagatctatt ccgggaccgc ttttagtagc ccgagcttta ttagtgtgat
      961 ggaggtgctg aaagaccgta attgtgatga gaacagtgag gtctggcaac gtgttcatgt
     1021 ggtgtatagc ctgtccaaag acctgggact gcctggtttt cgtgttggcg ccatctatag
     1081 caacgatgac atggttgtgg ctgccgctac aaaaatgagc agcttcggtc tggtttcttc
     1141 tcaaacccaa catctgctgt cagcaatgct gtccgataaa aaactgacca aaaactatat
     1201 cgccgagaat cataaacgcc tgaaacaacg ccaaaaaaaa ctggtgtctg gcctgcaaaa
     1261 atctggtatc agttgcctga acggtaatgc tggcctgttt tgctgggtcg acatgcgtca
     1321 cctgctgcgt tctaacacat tcgaggccga aatggaactg tggaaaaaaa tcgtgtatga
     1381 ggttcatctg aacattagcc cgggttcttc atgccactgt actgaaccgg gttggtttcg
     1441 tgtgtgtttc gccaatctgc ctgagcgtac tctggatctg gctatgcaac gtctgaaagc
     1501 cttcgtgggc gaataatact agagaaagag gagaaatact agatggaaaa cttcccgatc
     1561 atcaacctgg agaaactgaa tggtgacgaa cgtgccaata caatggaaat gattaaagac
     1621 gcctgtgaga actggggctt ttttgaactg gtgaaccacg gtatccctca tgaagtgatg
     1681 gacacggtcg agaaaatgac caaaggccac tataaaaaat gtatggagca gcgctttaaa
     1741 gagctggttg ccagcaaagg tctggaggct gttcaggcag aagtgaccga tctggattgg
     1801 gagagcacct tctttctgcg tcatctgccg accagtaaca ttagtcaggt gccggacctg
     1861 gatgaagaat atcgtgaggt catgcgcgat ttcgccaaac gcctggaaaa actggccgaa
     1921 gaactgctgg atctgctgtg tgaaaatctg gggctggaaa aaggttatct gaaaaacgcc
     1981 ttctatggct ctaaaggacc gaattttggg acgaaagtca gcaattatcc gccttgtccg
     2041 aaaccggatc tgatcaaagg actgcgtgcc cacaccgatg ccggtggtat tatcctgctg
     2101 tttcaagacg acaaagtgtc aggcctgcaa ctgctgaaag acgaacagtg gatcgatgtt
     2161 cctcctatgc gccactctat tgtggtgaat ctgggtgacc aactggaggt tattacgaac
     2221 ggaaaatata aaagtgtgct gcaccgcgtt attgctcaga cagatggcac ccgtatgagt
     2281 ctggcatcat tctataatcc gggctctgat gctgtaatct atccggccaa aacgctggta
     2341 gaaaaagagg ccgaggagtc tacacaggtt tatccgaaat ttgtgttcga cgactatatg
     2401 aaactgtatg ccgggctgaa attccaagca aaagaacctc gcttcgaagc catgaaagca
     2461 atggagagcg atccgattgc tagtgcctaa tactagagcc aggcatcaaa taaaacgaaa
     2521 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2581 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//