BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K417001 1 ACCsynth 1-aminocyclopropane-1-carboxylate (ACC) synthase 2010-10-19T11:00:00Z 2015-05-08T01:12:28Z Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet). (S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine). This enzyme is commonly referred to as ACC synthase. false false _537_ 0 6513 9 Not in stock false Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity. false Ben Porebski, Andrew Perry annotation2090376 1 ACC Synth range2090376 1 1 1290 annotation2090377 1 Stop range2090377 1 1288 1290 annotation2090375 1 Start range2090375 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K417007 1 BBa_K417007 Simple inducible (LacI) ethylene generator 2010-10-25T11:00:00Z 2015-05-08T01:12:28Z See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences. A simpler version of BBa_K417006 which expresses ACC synthase and ACC oxidase (EFE) to produce ethylene. This version does not express the SAM synthetase enzyme (BBa_K417000) and relies on S-adenosyl methionine generated by the chassis. false false _537_ 0 6167 9 Not in stock false Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device. false Ben Porebski, Andrew Perry component2103056 1 BBa_B0034 component2103050 1 BBa_B0034 component2103063 1 BBa_B0012 component2103061 1 BBa_B0010 component2103042 1 BBa_R0010 component2103054 1 BBa_K417001 component2103060 1 BBa_K417002 annotation2103050 1 BBa_B0034 range2103050 1 209 220 annotation2103042 1 BBa_R0010 range2103042 1 1 200 annotation2103054 1 BBa_K417001 range2103054 1 227 1516 annotation2103056 1 BBa_B0034 range2103056 1 1525 1536 annotation2103063 1 BBa_B0012 range2103063 1 2587 2627 annotation2103061 1 BBa_B0010 range2103061 1 2499 2578 annotation2103060 1 BBa_K417002 range2103060 1 1543 2490 BBa_K417002 1 ACCoxidase 1-aminocyclopropane-1-carboxylate (ACC) oxidase 2010-10-19T11:00:00Z 2015-05-08T01:12:28Z Solanum lycopersicum (Tomato) (Lycopersicon esculentum) codon optimized for <i>E. coli</i>. This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, which catalyses the formation of ethylene from 1-aminocyclopropane-1-carboxylate under the presence of ascorbate and O2. (1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O) This enzyme is commonly referred to as ACC oxidase or ethylene forming enzyme (EFE). false false _537_ 0 6513 9 Not in stock false Codon optimization for <i>E. coli</i> and removal of biobrick restriction sites. false Ben Porebski, Andrew Perry annotation2090378 1 Start range2090378 1 1 3 annotation2090379 1 ACC Oxidase range2090379 1 1 948 annotation2090380 1 Stop range2090380 1 946 948 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K417002_sequence 1 atggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaa BBa_K417007_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataatactagagaaagaggagaaatactagatggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K417001_sequence 1 atgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z