BBa_K417007
type: ComponentDefinition
persistent identity: BBa K417007
display id: BBa_K417007
version: 1
was generated by: iGEM to SBOL conversion
description: Simple inducible (LacI) ethylene generator
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:28Z
mutable provenance: See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences.
owned by: james
owned by: myers
mutable description: A simpler version of BBa_K417006 which expresses ACC synthase and ACC oxidase (EFE) to produce ethylene. This version does not express the SAM synthetase enzyme (BBa_K417000) and relies on S-adenosyl methionine generated by the chassis.
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device.
star: false
creator: Ben Porebski, Andrew Perry
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0012
component: BBa_B0010
component: BBa_K417002
component: BBa_B0034
component: BBa_K417001
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K417001
sequence annotation: BBa_K417002
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K417007_sequence/1

BBa_B0012
type: Component
persistent identity: component2103063
display id: component2103063
version: 1
top level: BBa_K417007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2103061
display id: component2103061
version: 1
top level: BBa_K417007
definition: BBa_B0010
access: public

BBa_K417002
type: Component
persistent identity: component2103060
display id: component2103060
version: 1
top level: BBa_K417007
definition: ACCoxidase
access: public

BBa_B0034
type: Component
persistent identity: component2103056
display id: component2103056
version: 1
top level: BBa_K417007
definition: BBa_B0034
access: public

BBa_K417001
type: Component
persistent identity: component2103054
display id: component2103054
version: 1
top level: BBa_K417007
definition: ACCsynth
access: public

BBa_B0034
type: Component
persistent identity: component2103050
display id: component2103050
version: 1
top level: BBa_K417007
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2103042
display id: component2103042
version: 1
top level: BBa_K417007
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2103042
display id: annotation2103042
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103042/range2103042/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K417007/annotation2103042/range2103042/1
type: Range
persistent identity: range2103042
display id: range2103042
version: 1
top level: BBa_K417007
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103050
display id: annotation2103050
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103050/range2103050/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K417007/annotation2103050/range2103050/1
type: Range
persistent identity: range2103050
display id: range2103050
version: 1
top level: BBa_K417007
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K417001
type: SequenceAnnotation
persistent identity: annotation2103054
display id: annotation2103054
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103054/range2103054/1
component: BBa_K417001

https://synbiohub.org/public/igem/BBa_K417007/annotation2103054/range2103054/1
type: Range
persistent identity: range2103054
display id: range2103054
version: 1
top level: BBa_K417007
direction: direction/reverse
start: 227
end: 1516
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103056
display id: annotation2103056
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103056/range2103056/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K417007/annotation2103056/range2103056/1
type: Range
persistent identity: range2103056
display id: range2103056
version: 1
top level: BBa_K417007
direction: direction/reverse
start: 1525
end: 1536
orientation: inline

BBa_K417002
type: SequenceAnnotation
persistent identity: annotation2103060
display id: annotation2103060
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103060/range2103060/1
component: BBa_K417002

https://synbiohub.org/public/igem/BBa_K417007/annotation2103060/range2103060/1
type: Range
persistent identity: range2103060
display id: range2103060
version: 1
top level: BBa_K417007
direction: direction/reverse
start: 1543
end: 2490
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2103061
display id: annotation2103061
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103061/range2103061/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K417007/annotation2103061/range2103061/1
type: Range
persistent identity: range2103061
display id: range2103061
version: 1
top level: BBa_K417007
direction: direction/forward
start: 2499
end: 2578
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2103063
display id: annotation2103063
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103063/range2103063/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K417007/annotation2103063/range2103063/1
type: Range
persistent identity: range2103063
display id: range2103063
version: 1
top level: BBa_K417007
direction: direction/forward
start: 2587
end: 2627
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ACCsynth
type: ComponentDefinition
persistent identity: BBa K417001
display id: BBa_K417001
version: 1
was generated by: iGEM to SBOL conversion
description: 1-aminocyclopropane-1-carboxylate (ACC) synthase
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
mutable provenance: Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed
owned by: james
owned by: myers
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet). (S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine). This enzyme is commonly referred to as ACC synthase.
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity.
star: false
creator: Ben Porebski, Andrew Perry
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ACC Synth
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K417001_sequence/1

ACC Synth
type: SequenceAnnotation
persistent identity: annotation2090376
display id: annotation2090376
version: 1
top level: ACCsynth
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1
type: Range
persistent identity: range2090376
display id: range2090376
version: 1
top level: ACCsynth
direction: direction/forward
start: 1
end: 1290
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2090375
display id: annotation2090375
version: 1
top level: ACCsynth
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1
type: Range
persistent identity: range2090375
display id: range2090375
version: 1
top level: ACCsynth
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2090377
display id: annotation2090377
version: 1
top level: ACCsynth
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1
type: Range
persistent identity: range2090377
display id: range2090377
version: 1
top level: ACCsynth
direction: direction/forward
start: 1288
end: 1290
orientation: inline

ACCoxidase
type: ComponentDefinition
persistent identity: BBa K417002
display id: BBa_K417002
version: 1
was generated by: iGEM to SBOL conversion
description: 1-aminocyclopropane-1-carboxylate (ACC) oxidase
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
mutable provenance: Solanum lycopersicum (Tomato) (Lycopersicon esculentum) codon optimized for E. coli.
owned by: james
owned by: myers
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, which catalyses the formation of ethylene from 1-aminocyclopropane-1-carboxylate under the presence of ascorbate and O2. (1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O) This enzyme is commonly referred to as ACC oxidase or ethylene forming enzyme (EFE).
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimization for E. coli and removal of biobrick restriction sites.
star: false
creator: Ben Porebski, Andrew Perry
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ACC Oxidase
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K417002_sequence/1

ACC Oxidase
type: SequenceAnnotation
persistent identity: annotation2090379
display id: annotation2090379
version: 1
top level: ACCoxidase
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1
type: Range
persistent identity: range2090379
display id: range2090379
version: 1
top level: ACCoxidase
direction: direction/forward
start: 1
end: 948
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2090378
display id: annotation2090378
version: 1
top level: ACCoxidase
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1
type: Range
persistent identity: range2090378
display id: range2090378
version: 1
top level: ACCoxidase
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2090380
display id: annotation2090380
version: 1
top level: ACCoxidase
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1
type: Range
persistent identity: range2090380
display id: range2090380
version: 1
top level: ACCoxidase
direction: direction/forward
start: 946
end: 948
orientation: inline

https://synbiohub.org/public/igem/BBa_K417007_sequence/1
type: Sequence
persistent identity: BBa K417007 sequence
display id: BBa_K417007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataatactagagaaagaggagaaatactagatggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K417001_sequence/1
type: Sequence
persistent identity: BBa K417001 sequence
display id: BBa_K417001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctgtctcgtaatgccaccttcaatagccatggtcaggactcttcgtattttctgggttggcaggagtatgaaaaaaacccttatcacgaggtccataacaccaacggcatcatccaaatgggcctggctgagaatcaactgtgctttgacctgctggaatcttggctggccaaaaatccggaagcagctgccttcaaaaaaaacggcgagagcattttcgctgaactggccctgtttcaagactatcatgggctgcctgagttcaaaaaagccatggtggactttatggccgaaattcgtgggaacaaagtgacctttgacccgaatcatctggttctgactgctggtgctacaagtgctaacgaaacgttcatcttttgtctggcagatcctggtgaagctgtactgatcccgacaccgtattatccaggcttcgatcgtgacctgaaatggcgtacaggtgtggaaatcgtaccgatccattgtaccagctctaatggtttccagatcacggaaacggcactggaagaagcatatcaggaggccgagaaacgtaatctgcgcgtgaaaggtgtactggtgaccaatccgtctaatcctctgggtactactatgacccgtaacgaactgtatctgctgctgtcctttgtggaggataaaggcattcacctgattagcgacgagatctattccgggaccgcttttagtagcccgagctttattagtgtgatggaggtgctgaaagaccgtaattgtgatgagaacagtgaggtctggcaacgtgttcatgtggtgtatagcctgtccaaagacctgggactgcctggttttcgtgttggcgccatctatagcaacgatgacatggttgtggctgccgctacaaaaatgagcagcttcggtctggtttcttctcaaacccaacatctgctgtcagcaatgctgtccgataaaaaactgaccaaaaactatatcgccgagaatcataaacgcctgaaacaacgccaaaaaaaactggtgtctggcctgcaaaaatctggtatcagttgcctgaacggtaatgctggcctgttttgctgggtcgacatgcgtcacctgctgcgttctaacacattcgaggccgaaatggaactgtggaaaaaaatcgtgtatgaggttcatctgaacattagcccgggttcttcatgccactgtactgaaccgggttggtttcgtgtgtgtttcgccaatctgcctgagcgtactctggatctggctatgcaacgtctgaaagccttcgtgggcgaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K417002_sequence/1
type: Sequence
persistent identity: BBa K417002 sequence
display id: BBa_K417002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaaaacttcccgatcatcaacctggagaaactgaatggtgacgaacgtgccaatacaatggaaatgattaaagacgcctgtgagaactggggcttttttgaactggtgaaccacggtatccctcatgaagtgatggacacggtcgagaaaatgaccaaaggccactataaaaaatgtatggagcagcgctttaaagagctggttgccagcaaaggtctggaggctgttcaggcagaagtgaccgatctggattgggagagcaccttctttctgcgtcatctgccgaccagtaacattagtcaggtgccggacctggatgaagaatatcgtgaggtcatgcgcgatttcgccaaacgcctggaaaaactggccgaagaactgctggatctgctgtgtgaaaatctggggctggaaaaaggttatctgaaaaacgccttctatggctctaaaggaccgaattttgggacgaaagtcagcaattatccgccttgtccgaaaccggatctgatcaaaggactgcgtgcccacaccgatgccggtggtattatcctgctgtttcaagacgacaaagtgtcaggcctgcaactgctgaaagacgaacagtggatcgatgttcctcctatgcgccactctattgtggtgaatctgggtgaccaactggaggttattacgaacggaaaatataaaagtgtgctgcaccgcgttattgctcagacagatggcacccgtatgagtctggcatcattctataatccgggctctgatgctgtaatctatccggccaaaacgctggtagaaaaagaggccgaggagtctacacaggtttatccgaaatttgtgttcgacgactatatgaaactgtatgccgggctgaaattccaagcaaaagaacctcgcttcgaagccatgaaagcaatggagagcgatccgattgctagtgcctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z