LOCUS       BBa_K418000             1416 bp    DNA     linear       30-Apr-2024
DEFINITION  IPTG inducible Lac promoter cassette
ACCESSION   BBa_K418000
VERSION     BBa_K418000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    50..53
                     /label=conserved
     RBS             44..57
                     /label=BBa_B0031
     misc_feature    1153..1191
                     /label=LVA
     CDS             64..1191
                     /label=lacI-LVA
     misc_feature    64..1191
                     /label=BBa_C0012
     misc_feature    1192..1216
                     /label=Help:Barcodes
     CDS             64..1191
                     /label=BBa_C0012
     stem_loop       1236..1279
                     /label=stem_loop
     misc_feature    1225..1304
                     /label=BBa_B0010
     terminator      1225..1304
                     /label=BBa_B0010
     stem_loop       1320..1339
                     /label=T7 TE
     misc_feature    1346..1346
                     /label=stop
     polyA_site      1340..1353
                     /label=polya
     misc_feature    1313..1353
                     /label=BBa_B0012
     terminator      1313..1353
                     /label=BBa_B0012
     terminator      1225..1353
                     /label=BBa_B0015
     misc_feature    1364..1380
                     /label=lac O1
     promoter        1381..1386
                     /label=-35
     misc_feature    1387..1403
                     /label=lac O1
     promoter        1404..1409
                     /label=-10
     misc_feature    1362..1415
                     /label=BBa_R0011
     promoter        1362..1415
                     /label=BBa_R0011
     misc_feature    44..1416
                     /label=BBa_Q01121
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggaaacctac
       61 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      121 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
      181 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
      241 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
      301 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
      361 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
      421 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
      481 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
      541 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
      601 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
      661 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
      721 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
      781 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
      841 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
      901 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
      961 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     1021 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1141 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1201 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1321 gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag cggataacaa
     1381 ttgacattgt gagcggataa caagatactg agcaca
//