LOCUS BBa_K422015 6827 bp DNA linear 03-Apr-2025 DEFINITION pSEVA132 ACCESSION BBa_K422015 VERSION BBa_K422015.1 FEATURES Location/Qualifiers promoter 309..1199 /label=araC CDS 1540..3471 /label=Insert flanked by two AarI sites (A- and C-part compatible misc_feature 3968..5337 /label=BBR1 ori CDS 5707..6687 /label=Ampicillin resistance promoter 1475..1501 /label=Arabinose Promoter ORIGIN 1 actagttcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 61 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 121 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 181 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 241 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 301 ctcggtaccc gggcgattta ttatgacaac ttgacggcta catcattcac tttttcttca 361 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa 421 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg 481 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc 541 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg 601 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg 661 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt 721 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg 781 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga 841 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc 901 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga 961 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc 1021 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc 1081 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc 1141 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata 1201 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc 1261 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa 1321 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat 1381 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata 1441 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact 1501 gtgggaattc gagctctaag gaggcacctg caaaaggaga tgaaaatcga agaaggtaaa 1561 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa 1621 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa 1681 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc 1741 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag 1801 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac 1861 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca 1921 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg 1981 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt 2041 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct 2101 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca 2161 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc 2221 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta 2281 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt 2341 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact 2401 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct 2461 tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa cgcccagaaa 2521 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg 2581 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact 2641 aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg 2701 ggagaaaatc tttattttca aggtcatcat catcatcatc atcatggaga aagcttgttt 2761 aaggggccgc gtgattacaa cccgatatcg agcaccattt gtcatttgac gaatgaatct 2821 gatgggcaca caacatcgtt gtatggtatt ggatttggtc ccttcatcat tacaaacaag 2881 cacttgttta gaagaaataa tggaacactg ttggtccaat cactacatgg tgtattcaag 2941 gtcaagaaca ccacgacttt gcaacaacac ctcattgatg ggagggacat gataattatt 3001 cgcatgccta aggatttccc accatttcct caaaagctga aatttagaga gccacaaagg 3061 gaagagcgca tatgtcttgt gacaaccaac ttccaaacta agagcatgtc tagcatggtg 3121 tcagacacta gttgcacatt cccttcatct gatggcatat tctggaagca ttggattcaa 3181 accaaggatg ggcagtgtgg cagtccatta gtatcaacta gagatgggtt cattgttggt 3241 atacactcag catcgaattt caccaacaca aacaattatt tcacaagcgt gccgaaaaac 3301 ttcatggaat tgttgacaaa tcaggaggcg cagcagtggg ttagtggttg gcgattaaat 3361 gctgactcag tattgtgggg gggccataaa gttttcatgg tgaaacctga agagcctttt 3421 cagccagtta aggaagcgac tcaactcatg aatcgtcgtc gccgtcgcta atgcgtttct 3481 agattgcagg tggaagcttc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3541 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3601 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 3661 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3721 tgcgtattgg gcgctcttcc gcttcttaat taaaggcatc aaataaaacg aaaggctcag 3781 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg 3841 acaaatccgc cgccctagac agctgggcgc gcccccccta cgggcttgct ctccgggctt 3901 cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg atggccgcga 3961 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg 4021 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt 4081 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt 4141 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac 4201 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc 4261 gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct 4321 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc 4381 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg 4441 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga 4501 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac 4561 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt 4621 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct 4681 gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac 4741 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga 4801 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct 4861 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac 4921 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt 4981 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt 5041 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag 5101 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct 5161 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga 5221 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac 5281 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct 5341 ggacgatggc gagccgttgg agccgccgac acgggtaacg ctgccgcgcc ggtagggccg 5401 gcctacggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg 5461 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga 5521 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt 5581 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca 5641 gcggaaaagg acaagggtcg tccaaaaaaa aaggctccaa aaggagcctt taattgtatc 5701 ggtttaccaa tgtttaatca ggctcgcgcc aatttccgca atctgacggt tacgttcatc 5761 catcgtcgcc tggctgccgg tggtataaat caccacaata cggctcggtt taccatccgg 5821 gcccagcgcc gcaataatgc cacggctgcc acgttcgccc gcaccgcttt tatccgcaat 5881 aaaccagcca gccggcagcg cgctacgcag cagcggaccc gccactttat ccgcttccat 5941 ccaatcaatc agttgctggc ggcttgccag ggtcagcagc tcacccgtca gcagtttacg 6001 cagggtggtc gccattgctg ccggcatggt ggtatcacgt tcatcgttcg gaatcgcttc 6061 gttcagttcc ggttcccaac gatccagacg ggtcacatga tcgcccatgt tatgcagaaa 6121 cgcggtcagt tctttcggac cgccaatggt cgtcagcagc aggttcgccg cggtgttatc 6181 gctcatggta atcgccgcgc tgcacagttc acgcacggtc atgccatcgg tcagatgttt 6241 ttcggtcacc gggctatatt ccaccagatc gttctggcta taatgaatac gacggcccag 6301 ttgttcctgg cccgcatcca cacggctcag aaccgcaccg cacagcagca ctttaaaggt 6361 gctcatcatc ggaaaacgtt cttccggacg aaaagattcc agaattttgc cgctgttcag 6421 atccagttca atatagccca cgcgcgcacc cagttgatct tccgcatctt tcactttcac 6481 cagggtttcc ggatgcgcaa acaccggcag gcaaaacgcc gcaaaaaacg gaatcagcgc 6541 cacacgaaaa tgctgaatgc tcatactctt cctttttcaa tattattgaa gcatttatca 6601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 6661 ggaattaaaa aaaagcccgc tcattaggcg ggctactaat ttaaatcgta attattgggg 6721 acccctggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc 6781 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaag //