BBa_K424003
1
BBa_K424003
luxC for light production protein generator
2010-06-06T11:00:00Z
2015-05-08T01:12:28Z
Uses part <partinfo>BBa_K424002</partinfo>
What does the device do? Helps to produce light
What are the inputs and outputs to the device? Takes in a long-chain aldehyde + CoA + NADP(+) and produces a long-chain acyl-CoA + NADPH.
Are any other devices needed? Also requires the other genes of the bacterial luciferase pathway.
Does the device have any chassis dependencies?
true
false
_545_
0
6272
9
Discontinued
false
This device uses a strong RBS to drive translation of luxC.
false
Patrick Nee
component2070301
1
BBa_K424002
component2070296
1
BBa_B0030
annotation2070296
1
BBa_B0030
range2070296
1
1
15
annotation2070301
1
BBa_K424002
range2070301
1
24
272
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K424002
1
BBa_K424002
LuxC gene from V. fischeri for generating light.
2010-06-06T11:00:00Z
2015-05-08T01:12:28Z
NCIB ACCESSION Y00509 REGION: 1624..1866
The luxC gene sequence from "V. fischeri luxR, luxI and luxC genes". This will generate light using the standard e coli metabolites. It does not require the addtion of any substrate.
true
false
_545_
0
6272
9
Discontinued
false
We had difficulty combining this part with BBa_ak4q343 due to XXXX.
false
Patrick Nee
annotation2070292
1
Stop Codon
range2070292
1
244
249
annotation2070294
1
light
range2070294
1
22
234
annotation2070293
1
start
range2070293
1
14
19
BBa_K424003_sequence
1
attaaagaggagaaatactagagataggttcgtcgccgattatattcttcacttttccatcgttgaccaacggtatataaaaaattcacaatctgatttaaatttagattaattctatctttgatatttaatgtttttgaaactgaactttcagtaatgacagataattttactctattatcattatttagtttaacttctttatatgcataattatcaaaatcttgaatcattccattaattatcattggaatacatttattcattaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K424002_sequence
1
ataggttcgtcgccgattatattcttcacttttccatcgttgaccaacggtatataaaaaattcacaatctgatttaaatttagattaattctatctttgatatttaatgtttttgaaactgaactttcagtaatgacagataattttactctattatcattatttagtttaacttctttatatgcataattatcaaaatcttgaatcattccattaattatcattggaatacatttattcattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z