##gff-version 3 ##sequence-region BBa_K424017 1 3348 BBa_K424017 . terminator 3220 3299 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K424017 . engineered_region 3220 3299 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K424017 . stem_loop 3231 3274 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K424017 . engineered_region 52 2485 . + 0 ID=BBa_K424018;Name=BBa_K424018 BBa_K424017 . CDS 1105 2385 . + 0 ID=annotation2086144;Name=RhlB;Parent=BBa_K424018 BBa_K424017 . stop_codon 2483 2485 . + 0 ID=annotation2086149;Name=end codon alanine;Parent=BBa_K424018 BBa_K424017 . point_mutation 1894 1899 . + 0 ID=annotation2086146;Name=silent mutation;Parent=BBa_K424018 BBa_K424017 . point_mutation 2008 2013 . + 0 ID=annotation2086147;Name=silent mutation;Parent=BBa_K424018 BBa_K424017 . engineered_region 152 2385 . + 0 ID=annotation2086159;Name=RhlAB Panama;Parent=BBa_K424018 BBa_K424017 . start_codon 52 54 . + 0 ID=annotation2086148;Name=start codon cysteine;Parent=BBa_K424018 BBa_K424017 . point_mutation 871 876 . + 0 ID=annotation2086145;Name=silent mutation;Parent=BBa_K424018 BBa_K424017 . sequence_feature 52 2485 . + 0 ID=annotation2086180;Name=Rhamnosyltransferase complex;Parent=BBa_K424018 BBa_K424017 . CDS 152 939 . + 0 ID=annotation2086143;Name=RhlA;Parent=BBa_K424018 BBa_K424017 . ribosome_entry_site 32 43 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K424017 . conserved_region 36 39 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K424017 . promoter 1 23 . + 0 ID=BBa_I719005;Name=BBa_I719005 BBa_K424017 . CDS 2492 3211 . + 0 ID=BBa_E0040;Name=BBa_E0040 BBa_K424017 . CDS 2492 3211 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 BBa_K424017 . terminator 3308 3348 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K424017 . polyA_site 3335 3348 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K424017 . engineered_region 3308 3348 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K424017 . stem_loop 3315 3334 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K424017 . stop_codon 3341 3341 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 >BBa_K424017 taatacgactcactatagggagatactagagaaagaggagaaatactagagtgtctgccgttcgaacaacaagaacgaa ctctacgtaatgccgggatacccgtggcagcgatagctgtttgcctgttcgaaaatttttgggaggtgtgaaatgcggc gcgaaagtctgttggtatcggtttgcaagggcctgcgggtacatgtcgagcgcgttgggcaggatcccgggcgcagcac ggtgatgctggtcaacggcgcgatggcgaccaccgcctcgttcgcccggacctgcaagtgcctggccgaacatttcaac gtggtgctgttcgacctgcccttcgccgggcagtcgcgtcagcacaacccgcagcgcgggttgatcaccaaggacgacg aggtggaaatcctcctggcgctgatcgagcgcttcgaggtcaatcacctggtctccgcgtcctggggcggtatctccac gctgctggcgctgtcgcgcaatccgcgcggcatccgcagctcggtggtgatggcattcgcccctggactgaaccaggcg atgctcgactacgtcgggcgggcgcaggcgctgatcgagctggacgacaagtcggcgatcggccatctgctcaacgaga ccgtcggcaaatacctgccgccgcgcctgaaagccagcaaccatcagcacatggcttcgctggccaccggcgaatacga gcaggcgcgctttcacatcgaccaggtgctggcgctcaacgatcggggctacctggcttgcctggagcggatccagagc cacgtgcatttcatcaacggcagctgggacgaatacaccaccgccgaggacgcccgccagttccgcgactacctgccgc actgtagtttctcgcgggtggagggcaccgggcatttcctcgacctggagtccaagctggccgcggtacgcgtgcaccg cgccctgctcgagcacctgctgaagcaaccggagccgcagcgggcggaacgcgcggcgggattccacgagatggccatc ggctacgcctgaacccttgacctgcgaagacccggcctggccgggctttgcggttgcataacgcacggagtagccccat gcacgccatcctcatcgccatcggctcggccggcgacgtatttcccttcatcggcttggcccggaccctgaaattgcgc gggcaccgcgtgagcctctgcaccatcccggtgtttcgcgacgcggtggagcagcacggcatcgcgttcgtcccgctga gcgacgaactgacctaccgccgggccatgggcgatccgcgcctgtgggaccccaagacgtccttcggcgtgctctggca agccatcgccgggatgatcgagccggtctacgagtacgtctcggcgcagcgccatgacgacatcgtggtggtcggctcg ctctgggcgctgggcgcacgcatcgctcacgagaagtacgggattccctacctgtccgcgcaggtctcgccatcgacct tgctgtcggcgcacctgccgccggtacaccccaagttcaacgtgcccgagcagatgccgctggcgatgcgcaagctgct ctggcgctgcatcgagcgcttcaagctggatcgcacctgcgcgccggagatcaacgcggtgcgccgcaaggtcggcctg gagacgccggtgaagcgcatcttcacccaatggatgcattcgccgcagggcgtggtctgcctgttcccggcctggttcg cgccaccccagcaggattggccgcaacccctgcacatgaccggcttcccgctgttcgacggcagtatcccggggacccc gctcgacgacgaactgcaacgctttctcgatcagggcagccggccgctggtgttcacccagggctcgaccgaacaccta cagggcgacttctacgccatggccctgcgcgcgctggaacgcctcggcgcgcgtgggatcttcctcaccggcgccggcc aggaaccgctgcgcggcttgccgaatcacgtgctacagcgcgcctacgcgccactgggagccttgctgccatcgtgcgc cgggctggtccatccgggcggtatcggcgccatgagcctggccttggcggcgggggtgccgcaggtgctgctgccctgc gcccacgaccagttcgacaatgccgaacggctggtccggctcggctgcgggatgcgcctgggcgtgccgttgcgcgagc aggagttgcgcggggcgctgtggcgcttgctcgaggacccggccatggcggcggcctgtcggcgtttcatggaattgtc acaaccgcacagtatcgcttgcggtaaagcggcccaggtggtcgaacgttgtcatagggagggggatgcgcgatggctg aaggctgcgtcctgaacggtgctggcataacagatagggttgccatgattttgccgtatcggcaaggctgcgcgcttga cagcgtcataccccgggccaattctgctgtgatgcatactagatgcgtaaaggagaagaacttttcactggagttgtcc caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt gtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacga aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct caccttcgggtgggcctttctgcgtttata