LOCUS       BBa_K426020             2910 bp    DNA     linear       01-Jul-2024
DEFINITION  Self-Lysis Device under Pbad and with BRP
ACCESSION   BBa_K426020
VERSION     BBa_K426020.1  
FEATURES             Location/Qualifiers
     promoter        1..1194
                     /label=Pbad
     CDS             1418..1919
                     /label=rbs.lambda lysozyme
     CDS             1920..2263
                     /label=rbs.lambda holin
     CDS             2440..2789
                     /label=rbs.antiholin 
     promoter        2404..2438
                     /label=Pcon
     CDS             1245..1417
                     /label=rbs.BRP
     stem_loop       2269..2397
                     /label=dbl Terminator
     stem_loop       2796..2908
                     /label=rnpB T1 Terminator
ORIGIN
        1 gatctctatg ctactccatc gagccgtcaa ttgtctgatt cgttaccaat tatgacaact
       61 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
      121 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
      181 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
      241 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
      301 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
      361 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
      421 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
      481 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
      541 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
      601 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
      661 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
      721 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
      781 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
      841 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
      901 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
      961 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     1021 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     1081 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     1141 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatctta
     1201 cctgacgctt tttatcgcaa ctctctactg tttctccata cccggatctt ttataaggag
     1261 gatctgtata tgaaagctac caaactggtt ctgggtgctg ttatcctggg ttctaccctg
     1321 ctggctgctt gccaggctaa ctacatccgt gacgttcagg gtcgtaccgt tgctccgtct
     1381 acctcttctg aactgaccgg tatcgctgtt cagtaaggat ctaaaaaagc cggagtagaa
     1441 gatggtagaa atcaataatc aacgtaaggc gttcctcgat atgctggcgt ggtcggaggg
     1501 aactgataac ggacgtcaga aaaccagaaa tcatggttat gacgtcattg taggcggaga
     1561 gctattcact gattactccg atcaccctcg caaacttgtc acgctaaacc caaaactcaa
     1621 atcaacaggc gccggacgct accagcttct ttcccgttgg tgggatgcct accgcaagca
     1681 gcttggcctg aaagacttct ctccgaaaag tcaggacgct gtggcattgc agcagattaa
     1741 ggagcgtggc gctttaccta tgattgatcg tggtgatatc cgtcaggcaa tcgaccgttg
     1801 cagcaatatc tgggcttcac tgccgggcgc tggttatggt cagttcgagc ataaggctga
     1861 cagcctgatt gcaaaattca aagaagcggg cggaacggtc agagagattg atgtatgagg
     1921 atctattggg ggtaagacct gaagatgcca gaaaaacatg acctgttggc cgccattctc
     1981 gcggcaaagg aacaaggcat cggggcaatc cttgcgtttg caatggcgta ccttcgcggc
     2041 agatataatg gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg cgccattatc
     2101 gcctggttca ttcgtgacct tctcgacttc gccggactaa gtagcaatct cgcttatata
     2161 acgagcgtgt ttatcggcta catcggtact gactcgattg gttcgcttat caaacgcttc
     2221 gctgctaaaa aagccggagt agaagatggt agaaatcaat aaggatctcc aggcatcaaa
     2281 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2341 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatagga
     2401 tctcttacgg ctagctcagt cctaggcact ttgctagcgg atctcccctt attgggggta
     2461 agacatgaag ctgccagaaa aacatgacct gttggccgcc attctcgcgg caaaggaaca
     2521 aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat ataatggcgg
     2581 tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct ggttcattcg
     2641 tgaccttctc gacttcgccg gactaagtag caatctcgct tatataacga gcgtgtttat
     2701 cggctacatc ggtactgact cgattggttc gcttatcaaa cgcttcgctg ctaaaaaagc
     2761 cggagtagaa gatggtagaa atcaataagg atctgccggc ttatcggtca gtttcacctg
     2821 atttacgtaa aaacccgctt cggcgggttt ttgcttttgg aggggcagaa agatgaatga
     2881 ctgtccacga cgctataccc aaaagaaatg
//