BBa_K426020 1 BBa_K426020 Self-Lysis Device under Pbad and with BRP 2010-10-26T11:00:00Z 2015-05-08T01:12:29Z Synthetic<br> References:<br> iGEM 2010, UC Berkeley: http://2010.igem.org/Team:Berkeley/Project/Self_Lysis <br> iGEM 2008, UC Berkeley: http://2008.igem.org/Team:UC_Berkeley/LysisDevice The Self Lysis Device acts to degrade the bacterial membrane (inner membrane, peptidoglycan layer and outer membrane).<br> The Construct<br> Pbad promoter: allows for fast, controlled induction, allows better control of the timing of lysis, addresses biosafety concerns since this promoter is only induced by an exogenous molecule. <br> Bacteriocin Releasing Protein (BRP): degrades the outer membrane <br> Lysozyme: degrades the peptidoglycan layer <br> Holin: creates pores in the inner membrane <br> Anti-holin: threshold-gating system <br> (for more complete descriptions, see 2008 UC Berkeley iGEM team's wiki)<br> A variation of this part (that does not include BRP) was characterized in standard E. coli media, Luria Broth (LB), by the 2008 UC Berkeley iGEM team. In the Anderson lab, the construct was modified to include the BRP protein and has been extensively tested as a means of delivery in Mammalian cells. The 2010 Berkeley iGEM team, characterized the device in an entirely new setting: for use inside of Choanoflagellates.<br> Self-lysis can be assayed by measuring changes in optical density, or changes in the opaqueness of a solution. As lysis occurs, optical density decreases, meaning the solution of bacteria becomes more translucent. A TECAN machine was used to measure the change in optical density over time. The self-lysis device was tested in LB, TB, artificial sea water (ASW), and Cereal Grass Media(CGM). Tests showed that TB is the best media for lysis, followed by LB, CGM, high ratios of LB:ASW, low ratios of LB: ASW, and ASW. Also, macroscopic tests indicate that lysis occurs in spring water:LB ratios as high as 80:20. false false _541_ 0 7241 9 It's complicated true NA false Daniela Mehech annotation2114314 1 rnpB T1 Terminator range2114314 1 2796 2908 annotation2114312 1 rbs.BRP range2114312 1 1245 1417 annotation2114310 1 rbs.antiholin range2114310 1 2440 2789 annotation2114291 1 Pbad range2114291 1 1 1194 annotation2114301 1 rbs.lambda holin range2114301 1 1920 2263 annotation2114292 1 rbs.lambda lysozyme range2114292 1 1418 1919 annotation2114311 1 Pcon range2114311 1 2404 2438 annotation2114313 1 dbl Terminator range2114313 1 2269 2397 BBa_K426020_sequence 1 gatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatcttttataaggaggatctgtatatgaaagctaccaaactggttctgggtgctgttatcctgggttctaccctgctggctgcttgccaggctaactacatccgtgacgttcagggtcgtaccgttgctccgtctacctcttctgaactgaccggtatcgctgttcagtaaggatctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctattcactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgaggatctattgggggtaagacctgaagatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggatctcttacggctagctcagtcctaggcactttgctagcggatctccccttattgggggtaagacatgaagctgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaggatctgccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z