BBa_K427002
1
BBa_K427002
Mor protein of Mu bacteriophage
2010-10-21T11:00:00Z
2015-05-08T01:12:29Z
Synthetized from the reported sequence of Mor protein from Mu bacteriophage. It was optimized for its expression in E. coli.
Mor is a protein of the Mu bacteriophage. It is typically transcribed by the early promoter of the phage and it activates the middle promoter Pm. Since it is the activator of Pm it can be used alongside this promoter to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.
false
false
_544_
0
6602
9
It's complicated
false
We did an E. coli codon optimization of the sequence and added a ribosome binding site to the sequence before synthesis.
false
Jan Marte Contreras Ortiz
annotation2092366
1
MuMor
range2092366
1
19
405
annotation2092364
1
RBS
range2092364
1
1
12
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_K427006
1
BBa_K427006
MuMor Sensitivity tuner (PoPS->PoPS)
2010-10-21T11:00:00Z
2015-05-08T01:12:29Z
We built this part form Mor and Pm sequences of Mu bacetriophage and included the bidirectional terminator BBa_B0014 from the registry.
This is a sensitivity tuner created form the Mor protein and Pm promoter of the Mu bacteriophage it can be used to increase the PoPS of a construct. The Mor protein encoded in the first part of the construct is the activator of the Pm promoter, which does not begin transcription until the Mor protein binds to its consensus sequence and attracts the polymerase.
false
false
_544_
0
6602
9
It's complicated
true
No special design considerations
false
Jan Marte Contreras Ortiz
component2092433
1
BBa_K427003
component2092431
1
BBa_B0014
component2092424
1
BBa_K427002
annotation2092431
1
BBa_B0014
range2092431
1
414
508
annotation2092433
1
BBa_K427003
range2092433
1
517
587
annotation2092424
1
BBa_K427002
range2092424
1
1
405
BBa_K427003
1
BBa_K427003
Pm promoter of Mu bacteriophage
2010-10-21T11:00:00Z
2015-05-08T01:12:29Z
Synthetized from the reported sequence of Pm promoter from Mu bacteriophage.
Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
false
false
_544_
0
6602
9
It's complicated
false
No special considerations
false
Jan Marte Contreras Ortiz
annotation2092367
1
Pm
range2092367
1
1
71
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K427006_sequence
1
aaagaggagaaatactagatgactgaagacctgtttggtgatctgcaagacgataccattctggcacacctggataatcctgccgaggacacaagccgttttccggcgctgctggctgaactgaacgatctgctgcgtggtgagctgtctcgcctgggagttgaccctgcccattctctggagattgtcgtggccatttgtaaacatctgggcggaggccaagtttatattccacgtggacaggcactggacagtctgattcgtgacctgcgcatttggaacgatttcaacgggcgtaatgtgagtgaactgactacccgctatggagtgacttttaacaccgtgtataaagccattcgccgtatgcgtcgtctgaaatatcgccagtatcaaccgagcctgctgtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttctgtaaacagtaaagccggttaatccggctttttttacgtcctcaatatcctgtgatgaataaccgtac
BBa_K427002_sequence
1
aaagaggagaaatactagatgactgaagacctgtttggtgatctgcaagacgataccattctggcacacctggataatcctgccgaggacacaagccgttttccggcgctgctggctgaactgaacgatctgctgcgtggtgagctgtctcgcctgggagttgaccctgcccattctctggagattgtcgtggccatttgtaaacatctgggcggaggccaagtttatattccacgtggacaggcactggacagtctgattcgtgacctgcgcatttggaacgatttcaacgggcgtaatgtgagtgaactgactacccgctatggagtgacttttaacaccgtgtataaagccattcgccgtatgcgtcgtctgaaatatcgccagtatcaaccgagcctgctg
BBa_K427003_sequence
1
ttctgtaaacagtaaagccggttaatccggctttttttacgtcctcaatatcctgtgatgaataaccgtac
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z