LOCUS       BBa_K427007             1755 bp    DNA     linear       20-Nov-2024
DEFINITION  Ter PBadweak Mu C Ter Pmom GFP cassette
ACCESSION   BBa_K427007
VERSION     BBa_K427007.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     stem_loop       62..84
                     /label=stem_loop
     misc_feature    50..95
                     /label=BBa_B0011
     terminator      50..95
                     /label=BBa_B0011
     terminator      1..95
                     /label=BBa_B0014
     promoter        104..234
                     /label=promoter
     misc_feature    143..160
                     /label=AraI1
     misc_feature    164..181
                     /label=AraI2
     promoter        104..233
                     /label=BBa_K206001
     RBS             242..253
                     /label=RBS
     CDS             259..679
                     /label=MuC
     misc_feature    242..679
                     /label=BBa_K427001
     stem_loop       695..714
                     /label=T7 TE
     misc_feature    721..721
                     /label=stop
     polyA_site      715..728
                     /label=polya
     misc_feature    688..728
                     /label=BBa_B0012
     terminator      688..728
                     /label=BBa_B0012
     stem_loop       749..771
                     /label=stem_loop
     misc_feature    737..782
                     /label=BBa_B0011
     terminator      737..782
                     /label=BBa_B0011
     terminator      688..782
                     /label=BBa_B0014
     promoter        791..869
                     /label=Pmom
     promoter        791..869
                     /label=BBa_K427004
     misc_feature    242..869
                     /label=BBa_K427005
     RBS             878..892
                     /label=RBS-1\Strong
     RBS             885..889
                     /label=RBS
     misc_feature    878..892
                     /label=BBa_B0030
     RBS             878..892
                     /label=BBa_B0030
     CDS             899..1618
                     /label=GFP protein
     CDS             899..1618
                     /label=BBa_E0040
     stem_loop       1638..1681
                     /label=stem_loop
     misc_feature    1627..1706
                     /label=BBa_B0010
     terminator      1627..1706
                     /label=BBa_B0010
     stem_loop       1722..1741
                     /label=T7 TE
     misc_feature    1748..1748
                     /label=stop
     polyA_site      1742..1755
                     /label=polya
     misc_feature    1715..1755
                     /label=BBa_B0012
     terminator      1715..1755
                     /label=BBa_B0012
     misc_feature    878..1755
                     /label=BBa_E0840
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
       61 aaagccagat tattaatccg gcttttttat tattttacta gagacattga ttatttgcac
      121 ggcgtcacac tttgctatgc catagctttt ttatccataa gattagcgga tcctacctga
      181 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga
      241 gaaagaggag aaatactaga tgcaacatga cctgtttgag catgatccgg cgattcgtca
      301 gctgattggc catatcgaca acattccggc acctgaactg gaaagtcgct ggcctcgtag
      361 cgtggttgat ctgatcgatg ttctggagaa cgaactgaaa cgccaaaatg tgtctaaccc
      421 acgtgagctg gctcgtaaac aagcagttgc cctgtcttgc ttcctgggtg gacgtcaatt
      481 ctatatcccg tgtggcgaca cgatcctgac agcactgcgt gatgatctgc tgtattgcca
      541 gtttaatggc cgtaacatgg aagaactgcg ccgtcaatat cgtctgtctc agccacagat
      601 ttatcaaatc attgctcgcc agcgtaaact gcatacacgt cgccatcaac ctgacctgtt
      661 ctctccggaa acaccgaaat actagagtca cactggctca ccttcgggtg ggcctttctg
      721 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
      781 tttactagag ggtaatacag atcgattatg ccccaataac cacactcaac ccatgatgtt
      841 ttttaagata gtggcgaatt gatgcaaagt actagagatt aaagaggaga aatactagat
      901 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      961 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1021 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1081 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1141 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1201 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1261 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1321 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1381 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1441 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1501 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1561 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1741 ctttctgcgt ttata
//