BBa_K427007
type: ComponentDefinition
persistent identity: BBa K427007
display id: BBa_K427007
version: 1
was generated by: iGEM to SBOL conversion
description: Ter PBadweak Mu C Ter Pmom GFP cassette
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Biobricks
owned by: james
owned by: myers
mutable description: This part was used for the characterization of the Mu C sensitivity tuner. It is composed of seven biobricks starting with Ter PBadweak, which is the part created by British Columbia team in 2007 starts with a terminator and a promoter that respond to high concentrations of Arabinose. It is followed by sequence coding for C protein which inter activates the Pmom promoter and this enhance the PoPS activity. Then is followed by the GFPcassette sequence a biobrick.
discontinued: false
dominant: false
experience: experience/Works
group u list: _544_
m user id: 0
owner id: 6605
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Diana Ostos Rangel
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0840
component: BBa_K427005
component: BBa_K206001
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_K206001
sequence annotation: BBa_K427005
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K427007_sequence/1

BBa_E0840
type: Component
persistent identity: component2092850
display id: component2092850
version: 1
top level: BBa_K427007
definition: GFP genera
access: public

BBa_K427005
type: Component
persistent identity: component2092839
display id: component2092839
version: 1
top level: BBa_K427007
definition: BBa_K427005
access: public

BBa_K206001
type: Component
persistent identity: component2092826
display id: component2092826
version: 1
top level: BBa_K427007
definition: BBa_K206001
access: public

BBa_B0014
type: Component
persistent identity: component2092822
display id: component2092822
version: 1
top level: BBa_K427007
definition: BBa_B0014
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092822
display id: annotation2092822
version: 1
top level: BBa_K427007
location: https://synbiohub.org/public/igem/BBa_K427007/annotation2092822/range2092822/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427007/annotation2092822/range2092822/1
type: Range
persistent identity: range2092822
display id: range2092822
version: 1
top level: BBa_K427007
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2092826
display id: annotation2092826
version: 1
top level: BBa_K427007
location: https://synbiohub.org/public/igem/BBa_K427007/annotation2092826/range2092826/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K427007/annotation2092826/range2092826/1
type: Range
persistent identity: range2092826
display id: range2092826
version: 1
top level: BBa_K427007
direction: direction/reverse
start: 104
end: 233
orientation: inline

BBa_K427005
type: SequenceAnnotation
persistent identity: annotation2092839
display id: annotation2092839
version: 1
top level: BBa_K427007
location: https://synbiohub.org/public/igem/BBa_K427007/annotation2092839/range2092839/1
component: BBa_K427005

https://synbiohub.org/public/igem/BBa_K427007/annotation2092839/range2092839/1
type: Range
persistent identity: range2092839
display id: range2092839
version: 1
top level: BBa_K427007
direction: direction/reverse
start: 242
end: 869
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2092850
display id: annotation2092850
version: 1
top level: BBa_K427007
location: https://synbiohub.org/public/igem/BBa_K427007/annotation2092850/range2092850/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K427007/annotation2092850/range2092850/1
type: Range
persistent identity: range2092850
display id: range2092850
version: 1
top level: BBa_K427007
direction: direction/reverse
start: 878
end: 1755
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_K427005
type: ComponentDefinition
persistent identity: BBa K427005
display id: BBa_K427005
version: 1
was generated by: iGEM to SBOL conversion
description: MuC sensitivity tuner (PoPS->PoPS)
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: We built this part form C and Pmom sequences of Mu bacetriophage and included the bidirectional terminator BBa_B0014 from the registry.
owned by: james
owned by: myers
mutable description: This is a sensitivity tuner created form the C protein and Pmom promoter of the Mu bacteriophage it can be used to increase the PoPS of a construct. The C protein encoded in the first part of the construct is the activator of the Pmom promoter, which does not begin transcription until the C protein binds to its consensus sequence and attracts the polymerase.
discontinued: false
dominant: false
experience: experience/Works
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special design considerations
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K427001
component: BBa_K427004
component: BBa_B0014
sequence annotation: BBa_K427001
sequence annotation: BBa_B0014
sequence annotation: BBa_K427004
sequence: https://synbiohub.org/public/igem/BBa_K427005_sequence/1

BBa_K427001
type: Component
persistent identity: component2092375
display id: component2092375
version: 1
top level: BBa_K427005
definition: BBa_K427001
access: public

BBa_K427004
type: Component
persistent identity: component2092384
display id: component2092384
version: 1
top level: BBa_K427005
definition: BBa_K427004
access: public

BBa_B0014
type: Component
persistent identity: component2092382
display id: component2092382
version: 1
top level: BBa_K427005
definition: BBa_B0014
access: public

BBa_K427001
type: SequenceAnnotation
persistent identity: annotation2092375
display id: annotation2092375
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092375/range2092375/1
component: BBa_K427001

https://synbiohub.org/public/igem/BBa_K427005/annotation2092375/range2092375/1
type: Range
persistent identity: range2092375
display id: range2092375
version: 1
top level: BBa_K427005
direction: direction/reverse
start: 1
end: 438
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092382
display id: annotation2092382
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092382/range2092382/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427005/annotation2092382/range2092382/1
type: Range
persistent identity: range2092382
display id: range2092382
version: 1
top level: BBa_K427005
direction: direction/reverse
start: 447
end: 541
orientation: inline

BBa_K427004
type: SequenceAnnotation
persistent identity: annotation2092384
display id: annotation2092384
version: 1
top level: BBa_K427005
location: https://synbiohub.org/public/igem/BBa_K427005/annotation2092384/range2092384/1
component: BBa_K427004

https://synbiohub.org/public/igem/BBa_K427005/annotation2092384/range2092384/1
type: Range
persistent identity: range2092384
display id: range2092384
version: 1
top level: BBa_K427005
direction: direction/reverse
start: 550
end: 628
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD weak
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: Site-directed mutagenesis on <partinfo>I13453</partinfo> with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker version of wild type pBAD (<partinfo>I13453</partinfo>).
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
top level: BBa_K206001
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
top level: BBa_K206001
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
top level: BBa_K206001
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K427001
type: ComponentDefinition
persistent identity: BBa K427001
display id: BBa_K427001
version: 1
was generated by: iGEM to SBOL conversion
description: C protein of the Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of C protein from Mu bacteriophage. It was optimized for its expression in E. coli.
owned by: james
owned by: myers
mutable description: C is a protein of the Mu bacteriophage. It is typically transcribed by the middle promoter of the phage (Pm) and it activates the four late promoters Plys, Pi, Pp and Pmom. Since it is the activator of the late it can be used alongside them to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.
discontinued: false
dominant: false
experience: experience/None
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was optimized for its production on E. coli and the ribosome binding site was added at the beginning of the sequence.
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: MuC
sequence: https://synbiohub.org/public/igem/BBa_K427001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2092369
display id: annotation2092369
version: 1
top level: BBa_K427001
location: https://synbiohub.org/public/igem/BBa_K427001/annotation2092369/range2092369/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K427001/annotation2092369/range2092369/1
type: Range
persistent identity: range2092369
display id: range2092369
version: 1
top level: BBa_K427001
direction: direction/forward
start: 1
end: 12
orientation: inline

MuC
type: SequenceAnnotation
persistent identity: annotation2092370
display id: annotation2092370
version: 1
top level: BBa_K427001
location: https://synbiohub.org/public/igem/BBa_K427001/annotation2092370/range2092370/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K427001/annotation2092370/range2092370/1
type: Range
persistent identity: range2092370
display id: range2092370
version: 1
top level: BBa_K427001
direction: direction/forward
start: 18
end: 438
orientation: inline

BBa_K427004
type: ComponentDefinition
persistent identity: BBa K427004
display id: BBa_K427004
version: 1
was generated by: iGEM to SBOL conversion
description: Pmom promoter of Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of Pmom promoter from Mu bacteriophage
owned by: james
owned by: myers
mutable description: Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The design of this part had no special considerations
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pmom
sequence: https://synbiohub.org/public/igem/BBa_K427004_sequence/1

Pmom
type: SequenceAnnotation
persistent identity: annotation2092368
display id: annotation2092368
version: 1
top level: BBa_K427004
location: https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
type: Range
persistent identity: range2092368
display id: range2092368
version: 1
top level: BBa_K427004
direction: direction/forward
start: 1
end: 79
orientation: inline

https://synbiohub.org/public/igem/BBa_K427007_sequence/1
type: Sequence
persistent identity: BBa K427007 sequence
display id: BBa_K427007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgattcgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctgatcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccctgtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctgtattgccagtttaatggccgtaacatggaagaactgcgccgtcaatatcgtctgtctcagccacagatttatcaaatcattgctcgccagcgtaaactgcatacacgtcgccatcaacctgacctgttctctccggaaacaccgaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagggtaatacagatcgattatgccccaataaccacactcaacccatgatgttttttaagatagtggcgaattgatgcaaagtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427005_sequence/1
type: Sequence
persistent identity: BBa K427005 sequence
display id: BBa_K427005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgattcgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctgatcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccctgtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctgtattgccagtttaatggccgtaacatggaagaactgcgccgtcaatatcgtctgtctcagccacagatttatcaaatcattgctcgccagcgtaaactgcatacacgtcgccatcaacctgacctgttctctccggaaacaccgaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagggtaatacagatcgattatgccccaataaccacactcaacccatgatgttttttaagatagtggcgaattgatgcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206001_sequence/1
type: Sequence
persistent identity: BBa K206001 sequence
display id: BBa_K206001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427004_sequence/1
type: Sequence
persistent identity: BBa K427004 sequence
display id: BBa_K427004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtaatacagatcgattatgccccaataaccacactcaacccatgatgttttttaagatagtggcgaattgatgcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427001_sequence/1
type: Sequence
persistent identity: BBa K427001 sequence
display id: BBa_K427001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgattcgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctgatcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccctgtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctgtattgccagtttaatggccgtaacatggaagaactgcgccgtcaatatcgtctgtctcagccacagatttatcaaatcattgctcgccagcgtaaactgcatacacgtcgccatcaacctgacctgttctctccggaaacaccgaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z