##gff-version 3
##sequence-region BBa_K427009 1 5138
BBa_K427009	.	engineered_region	1034	1297	.	+	0	ID=BBa_I746352;Name=BBa_I746352
BBa_K427009	.	CDS	1055	1297	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K427009	.	start_codon	1055	1055	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K427009	.	sequence_alteration	1135	1135	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K427009	.	engineered_region	1034	1045	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_K427009	.	terminator	793	887	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K427009	.	terminator	793	833	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K427009	.	polyA_site	820	833	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	800	819	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	826	826	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	793	833	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	terminator	842	887	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K427009	.	engineered_region	842	887	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K427009	.	stem_loop	854	876	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K427009	.	terminator	2131	2225	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K427009	.	terminator	2131	2171	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K427009	.	polyA_site	2158	2171	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	2138	2157	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	2164	2164	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	2131	2171	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	terminator	2180	2225	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K427009	.	engineered_region	2180	2225	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K427009	.	stem_loop	2192	2214	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K427009	.	engineered_region	242	646	.	+	0	ID=BBa_K427002;Name=BBa_K427002
BBa_K427009	.	ribosome_entry_site	242	253	.	+	0	ID=annotation2092364;Name=RBS;Parent=BBa_K427002
BBa_K427009	.	CDS	260	646	.	+	0	ID=annotation2092366;Name=MuMor;Parent=BBa_K427002
BBa_K427009	.	promoter	1547	1676	.	+	0	ID=BBa_K206001;Name=BBa_K206001
BBa_K427009	.	promoter	1547	1677	.	+	0	ID=annotation2049255;Name=promoter;Parent=BBa_K206001
BBa_K427009	.	operator	1607	1624	.	+	0	ID=annotation2049257;Name=AraI2;Parent=BBa_K206001
BBa_K427009	.	operator	1586	1603	.	+	0	ID=annotation2049256;Name=AraI1;Parent=BBa_K206001
BBa_K427009	.	engineered_region	1685	2122	.	+	0	ID=BBa_K427001;Name=BBa_K427001
BBa_K427009	.	CDS	1702	2122	.	+	0	ID=annotation2092370;Name=MuC;Parent=BBa_K427001
BBa_K427009	.	ribosome_entry_site	1685	1696	.	+	0	ID=annotation2092369;Name=RBS;Parent=BBa_K427001
BBa_K427009	.	engineered_region	4270	5138	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_K427009	.	terminator	5098	5138	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K427009	.	polyA_site	5125	5138	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	5105	5124	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	5131	5131	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	5098	5138	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	engineered_region	4288	5001	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K427009	.	CDS	4288	4998	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K427009	.	sequence_alteration	4639	4639	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K427009	.	terminator	5010	5089	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K427009	.	stem_loop	5021	5064	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K427009	.	engineered_region	5010	5089	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K427009	.	ribosome_entry_site	4270	4281	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K427009	.	conserved_region	4274	4277	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K427009	.	terminator	1	95	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K427009	.	terminator	1	41	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K427009	.	polyA_site	28	41	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	8	27	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	34	34	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	1	41	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	terminator	50	95	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K427009	.	engineered_region	50	95	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K427009	.	stem_loop	62	84	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K427009	.	engineered_region	3297	4174	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_K427009	.	CDS	3315	4037	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_K427009	.	ribosome_entry_site	3297	3308	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_K427009	.	conserved_region	3301	3304	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K427009	.	terminator	4134	4174	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_K427009	.	polyA_site	4161	4174	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	4141	4160	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	4167	4167	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	4134	4174	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	terminator	4046	4125	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_K427009	.	stem_loop	4057	4100	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K427009	.	engineered_region	4046	4125	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K427009	.	promoter	4183	4261	.	+	0	ID=BBa_K427004;Name=BBa_K427004
BBa_K427009	.	promoter	4183	4261	.	+	0	ID=annotation2092368;Name=Pmom;Parent=BBa_K427004
BBa_K427009	.	promoter	3197	3288	.	+	0	ID=BBa_I746361;Name=BBa_I746361
BBa_K427009	.	promoter	3197	3288	.	+	0	ID=annotation1943784;Name=PO;Parent=BBa_I746361
BBa_K427009	.	promoter	104	233	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K427009	.	operator	164	181	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K427009	.	operator	143	160	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K427009	.	promoter	104	234	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K427009	.	engineered_region	2313	3188	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K427009	.	terminator	3148	3188	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K427009	.	polyA_site	3175	3188	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	3155	3174	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	3181	3181	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	3148	3188	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	CDS	2332	3051	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K427009	.	CDS	2332	3051	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K427009	.	terminator	3060	3139	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K427009	.	stem_loop	3071	3114	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K427009	.	engineered_region	3060	3139	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K427009	.	ribosome_entry_site	2313	2325	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K427009	.	engineered_region	2313	2325	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K427009	.	ribosome_entry_site	2319	2322	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K427009	.	ribosome_entry_site	2313	2325	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K427009	.	promoter	1306	1435	.	+	0	ID=BBa_K427000;Name=BBa_K427000
BBa_K427009	.	promoter	1306	1435	.	+	0	ID=annotation2090357;Name=PBad Weak Reverse;Parent=BBa_K427000
BBa_K427009	.	terminator	1444	1538	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K427009	.	terminator	1444	1484	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K427009	.	polyA_site	1471	1484	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K427009	.	stem_loop	1451	1470	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K427009	.	stop_codon	1477	1477	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K427009	.	engineered_region	1444	1484	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K427009	.	terminator	1493	1538	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K427009	.	engineered_region	1493	1538	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K427009	.	stem_loop	1505	1527	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K427009	.	promoter	896	1025	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K427009	.	promoter	2234	2304	.	+	0	ID=BBa_K427003;Name=BBa_K427003
BBa_K427009	.	promoter	2234	2304	.	+	0	ID=annotation2092367;Name=Pm;Parent=BBa_K427003
BBa_K427009	.	promoter	655	784	.	+	0	ID=BBa_J44002;Name=BBa_J44002
BBa_K427009	.	promoter	655	784	.	+	0	ID=annotation2002776;Name=promoter;Parent=BBa_J44002
>BBa_K427009
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatcc
ggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccat
aagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctact
agagaaagaggagaaatactagatgactgaagacctgtttggtgatctgcaagacgataccattctggcacacctggat
aatcctgccgaggacacaagccgttttccggcgctgctggctgaactgaacgatctgctgcgtggtgagctgtctcgcc
tgggagttgaccctgcccattctctggagattgtcgtggccatttgtaaacatctgggcggaggccaagtttatattcc
acgtggacaggcactggacagtctgattcgtgacctgcgcatttggaacgatttcaacgggcgtaatgtgagtgaactg
actacccgctatggagtgacttttaacaccgtgtataaagccattcgccgtatgcgtcgtctgaaatatcgccagtatc
aaccgagcctgctgtactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcag
gtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaat
ccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatcc
ataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcta
ctagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatg
tgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaat
ttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagtt
aaaggctgttacagctcaccattcaaccattaatactagaggctagcccaaaaaaacggtatggagaaacagtagagag
ttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaaagctatggcatagcaaagtgtgacgccgtgc
aaataatcaatgttactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatat
aaaaagccagattattaatccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgcta
tgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatac
cgtttttttgggctagctactagagaaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgatt
cgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctga
tcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccct
gtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctg
tattgccagtttaatggccgtaacatggaagaactgcgccgtcaatatcgtctgtctcagccacagatttatcaaatca
ttgctcgccagcgtaaactgcatacacgtcgccatcaacctgacctgttctctccggaaacaccgaaatactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggc
ttttttattattttactagagttctgtaaacagtaaagccggttaatccggctttttttacgtcctcaatatcctgtga
tgaataaccgtactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataag
ccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggtaa
tacagatcgattatgccccaataaccacactcaacccatgatgttttttaagatagtggcgaattgatgcaaagtacta
gagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttca
aggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcac
ccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtac
ggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtggg
agcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatcta
caaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcc
tccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccact
acgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagtt
ggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggc
atggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata