BBa_K427009
type: ComponentDefinition
persistent identity: BBa K427009
display id: BBa_K427009
version: 1
was generated by: iGEM to SBOL conversion
description: Intelligent arabinose biosensor with differential response
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: The parts used to build this device came from several sources. The proteins C and Mor, as well as the Pm and Pmom promoters come from slight modifications of the reported genome of the Mu bacteriophage. The pBad family of promoters comes from British Columbia's 2009 project and are modifications of the wt PBad promoter from E. coli. The other activator and promoter come from Cambridge's 2007 project while the fluorescent proteins and terminators come from the registry of parts.
owned by: james
owned by: myers
mutable description: This part was the final construct of the Tec-Monterrey iGEM Team in 2010. It senses the concentration of arabinose through the PBad family of promoters. Then amplifies it through sensitivity tuners, the activator proteins of the tuners are jammed by reverse arabinose promoters. The sensitivity tuners activate the production of the three fluorescent proteins. This device was created as a proof of concept for the combination of the jammers and sensitivity tuners, rather than using the whole part, the construct design is what should be regarded.
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There were no special considerations. This sequence is a proof of concept of the combination of the jammer and sensitivity tuner devices.
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_I13453
component: BBa_B0014
component: BBa_J44002
component: BBa_K427002
component: BBa_K206000
component: BBa_J06702
component: BBa_K427004
component: BBa_E0430
component: BBa_I746361
component: BBa_E0240
component: BBa_K427003
component: BBa_K427001
component: BBa_K206001
component: BBa_K427000
component: BBa_I746352
sequence annotation: BBa_B0014
sequence annotation: BBa_K206000
sequence annotation: BBa_K427002
sequence annotation: BBa_J44002
sequence annotation: BBa_I13453
sequence annotation: BBa_I746352
sequence annotation: BBa_K427000
sequence annotation: BBa_K206001
sequence annotation: BBa_K427001
sequence annotation: BBa_K427003
sequence annotation: BBa_E0240
sequence annotation: BBa_I746361
sequence annotation: BBa_E0430
sequence annotation: BBa_K427004
sequence annotation: BBa_J06702
sequence: https://synbiohub.org/public/igem/BBa_K427009_sequence/1

BBa_I13453
type: Component
persistent identity: component2092659
display id: component2092659
version: 1
top level: BBa_K427009
definition: BBa_I13453
access: public

BBa_B0014
type: Component
persistent identity: component2092658
display id: component2092658
version: 1
top level: BBa_K427009
definition: BBa_B0014
access: public

BBa_J44002
type: Component
persistent identity: component2092651
display id: component2092651
version: 1
top level: BBa_K427009
definition: BBa_J44002
access: public

BBa_K427002
type: Component
persistent identity: component2092649
display id: component2092649
version: 1
top level: BBa_K427009
definition: BBa_K427002
access: public

BBa_K206000
type: Component
persistent identity: component2092646
display id: component2092646
version: 1
top level: BBa_K427009
definition: pBAD
access: public

BBa_B0014
type: Component
persistent identity: component2092642
display id: component2092642
version: 1
top level: BBa_K427009
definition: BBa_B0014
access: public

BBa_J06702
type: Component
persistent identity: component2092726
display id: component2092726
version: 1
top level: BBa_K427009
definition: mCherry
access: public

BBa_K427004
type: Component
persistent identity: component2092714
display id: component2092714
version: 1
top level: BBa_K427009
definition: BBa_K427004
access: public

BBa_E0430
type: Component
persistent identity: component2092712
display id: component2092712
version: 1
top level: BBa_K427009
definition: BBa_E0430
access: public

BBa_I746361
type: Component
persistent identity: component2092702
display id: component2092702
version: 1
top level: BBa_K427009
definition: BBa_I746361
access: public

BBa_E0240
type: Component
persistent identity: component2092700
display id: component2092700
version: 1
top level: BBa_K427009
definition: GFP report
access: public

BBa_K427003
type: Component
persistent identity: component2092689
display id: component2092689
version: 1
top level: BBa_K427009
definition: BBa_K427003
access: public

BBa_B0014
type: Component
persistent identity: component2092687
display id: component2092687
version: 1
top level: BBa_K427009
definition: BBa_B0014
access: public

BBa_K427001
type: Component
persistent identity: component2092680
display id: component2092680
version: 1
top level: BBa_K427009
definition: BBa_K427001
access: public

BBa_K206001
type: Component
persistent identity: component2092677
display id: component2092677
version: 1
top level: BBa_K427009
definition: BBa_K206001
access: public

BBa_B0014
type: Component
persistent identity: component2092673
display id: component2092673
version: 1
top level: BBa_K427009
definition: BBa_B0014
access: public

BBa_K427000
type: Component
persistent identity: component2092666
display id: component2092666
version: 1
top level: BBa_K427009
definition: pBad Weak
access: public

BBa_I746352
type: Component
persistent identity: component2092664
display id: component2092664
version: 1
top level: BBa_K427009
definition: BBa_I746352
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092642
display id: annotation2092642
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092642/range2092642/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427009/annotation2092642/range2092642/1
type: Range
persistent identity: range2092642
display id: range2092642
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2092646
display id: annotation2092646
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092646/range2092646/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K427009/annotation2092646/range2092646/1
type: Range
persistent identity: range2092646
display id: range2092646
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 104
end: 233
orientation: inline

BBa_K427002
type: SequenceAnnotation
persistent identity: annotation2092649
display id: annotation2092649
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092649/range2092649/1
component: BBa_K427002

https://synbiohub.org/public/igem/BBa_K427009/annotation2092649/range2092649/1
type: Range
persistent identity: range2092649
display id: range2092649
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 242
end: 646
orientation: inline

BBa_J44002
type: SequenceAnnotation
persistent identity: annotation2092651
display id: annotation2092651
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092651/range2092651/1
component: BBa_J44002

https://synbiohub.org/public/igem/BBa_K427009/annotation2092651/range2092651/1
type: Range
persistent identity: range2092651
display id: range2092651
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 655
end: 784
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092658
display id: annotation2092658
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092658/range2092658/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427009/annotation2092658/range2092658/1
type: Range
persistent identity: range2092658
display id: range2092658
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 793
end: 887
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2092659
display id: annotation2092659
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092659/range2092659/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K427009/annotation2092659/range2092659/1
type: Range
persistent identity: range2092659
display id: range2092659
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 896
end: 1025
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2092664
display id: annotation2092664
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092664/range2092664/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K427009/annotation2092664/range2092664/1
type: Range
persistent identity: range2092664
display id: range2092664
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1034
end: 1297
orientation: inline

BBa_K427000
type: SequenceAnnotation
persistent identity: annotation2092666
display id: annotation2092666
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092666/range2092666/1
component: BBa_K427000

https://synbiohub.org/public/igem/BBa_K427009/annotation2092666/range2092666/1
type: Range
persistent identity: range2092666
display id: range2092666
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1306
end: 1435
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092673
display id: annotation2092673
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092673/range2092673/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427009/annotation2092673/range2092673/1
type: Range
persistent identity: range2092673
display id: range2092673
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1444
end: 1538
orientation: inline

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2092677
display id: annotation2092677
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092677/range2092677/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K427009/annotation2092677/range2092677/1
type: Range
persistent identity: range2092677
display id: range2092677
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1547
end: 1676
orientation: inline

BBa_K427001
type: SequenceAnnotation
persistent identity: annotation2092680
display id: annotation2092680
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092680/range2092680/1
component: BBa_K427001

https://synbiohub.org/public/igem/BBa_K427009/annotation2092680/range2092680/1
type: Range
persistent identity: range2092680
display id: range2092680
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 1685
end: 2122
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092687
display id: annotation2092687
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092687/range2092687/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K427009/annotation2092687/range2092687/1
type: Range
persistent identity: range2092687
display id: range2092687
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 2131
end: 2225
orientation: inline

BBa_K427003
type: SequenceAnnotation
persistent identity: annotation2092689
display id: annotation2092689
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092689/range2092689/1
component: BBa_K427003

https://synbiohub.org/public/igem/BBa_K427009/annotation2092689/range2092689/1
type: Range
persistent identity: range2092689
display id: range2092689
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 2234
end: 2304
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2092700
display id: annotation2092700
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092700/range2092700/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K427009/annotation2092700/range2092700/1
type: Range
persistent identity: range2092700
display id: range2092700
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 2313
end: 3188
orientation: inline

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2092702
display id: annotation2092702
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092702/range2092702/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K427009/annotation2092702/range2092702/1
type: Range
persistent identity: range2092702
display id: range2092702
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 3197
end: 3288
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2092712
display id: annotation2092712
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092712/range2092712/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K427009/annotation2092712/range2092712/1
type: Range
persistent identity: range2092712
display id: range2092712
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 3297
end: 4174
orientation: inline

BBa_K427004
type: SequenceAnnotation
persistent identity: annotation2092714
display id: annotation2092714
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092714/range2092714/1
component: BBa_K427004

https://synbiohub.org/public/igem/BBa_K427009/annotation2092714/range2092714/1
type: Range
persistent identity: range2092714
display id: range2092714
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 4183
end: 4261
orientation: inline

BBa_J06702
type: SequenceAnnotation
persistent identity: annotation2092726
display id: annotation2092726
version: 1
top level: BBa_K427009
location: https://synbiohub.org/public/igem/BBa_K427009/annotation2092726/range2092726/1
component: BBa_J06702

https://synbiohub.org/public/igem/BBa_K427009/annotation2092726/range2092726/1
type: Range
persistent identity: range2092726
display id: range2092726
version: 1
top level: BBa_K427009
direction: direction/reverse
start: 4270
end: 5138
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_E0430
type: ComponentDefinition
persistent identity: BBa E0430
display id: BBa_E0430
version: 1
was generated by: iGEM to SBOL conversion
description: EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Standard YFP Output Device -LVA tag
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0430_sequence/1

BBa_B0012
type: Component
persistent identity: component942902
display id: component942902
version: 1
top level: BBa_E0430
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component942892
display id: component942892
version: 1
top level: BBa_E0430
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component942887
display id: component942887
version: 1
top level: BBa_E0430
definition: eyfp
access: public

BBa_B0034
type: Component
persistent identity: component942885
display id: component942885
version: 1
top level: BBa_E0430
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942885
display id: annotation942885
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
type: Range
persistent identity: range942885
display id: range942885
version: 1
top level: BBa_E0430
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation942887
display id: annotation942887
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
type: Range
persistent identity: range942887
display id: range942887
version: 1
top level: BBa_E0430
direction: direction/forward
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942892
display id: annotation942892
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
type: Range
persistent identity: range942892
display id: range942892
version: 1
top level: BBa_E0430
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942902
display id: annotation942902
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
type: Range
persistent identity: range942902
display id: range942902
version: 1
top level: BBa_E0430
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_K427002
type: ComponentDefinition
persistent identity: BBa K427002
display id: BBa_K427002
version: 1
was generated by: iGEM to SBOL conversion
description: Mor protein of Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of Mor protein from Mu bacteriophage. It was optimized for its expression in E. coli.
owned by: james
owned by: myers
mutable description: Mor is a protein of the Mu bacteriophage. It is typically transcribed by the early promoter of the phage and it activates the middle promoter Pm. Since it is the activator of Pm it can be used alongside this promoter to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.
discontinued: false
dominant: false
experience: experience/None
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We did an E. coli codon optimization of the sequence and added a ribosome binding site to the sequence before synthesis.
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: MuMor
sequence: https://synbiohub.org/public/igem/BBa_K427002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2092364
display id: annotation2092364
version: 1
top level: BBa_K427002
location: https://synbiohub.org/public/igem/BBa_K427002/annotation2092364/range2092364/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K427002/annotation2092364/range2092364/1
type: Range
persistent identity: range2092364
display id: range2092364
version: 1
top level: BBa_K427002
direction: direction/forward
start: 1
end: 12
orientation: inline

MuMor
type: SequenceAnnotation
persistent identity: annotation2092366
display id: annotation2092366
version: 1
top level: BBa_K427002
location: https://synbiohub.org/public/igem/BBa_K427002/annotation2092366/range2092366/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K427002/annotation2092366/range2092366/1
type: Range
persistent identity: range2092366
display id: range2092366
version: 1
top level: BBa_K427002
direction: direction/forward
start: 19
end: 405
orientation: inline

BBa_K427003
type: ComponentDefinition
persistent identity: BBa K427003
display id: BBa_K427003
version: 1
was generated by: iGEM to SBOL conversion
description: Pm promoter of Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of Pm promoter from Mu bacteriophage.
owned by: james
owned by: myers
mutable description: Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No special considerations
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K427003_sequence/1

Pm
type: SequenceAnnotation
persistent identity: annotation2092367
display id: annotation2092367
version: 1
top level: BBa_K427003
location: https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1
type: Range
persistent identity: range2092367
display id: range2092367
version: 1
top level: BBa_K427003
direction: direction/forward
start: 1
end: 71
orientation: inline

BBa_K427004
type: ComponentDefinition
persistent identity: BBa K427004
display id: BBa_K427004
version: 1
was generated by: iGEM to SBOL conversion
description: Pmom promoter of Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of Pmom promoter from Mu bacteriophage
owned by: james
owned by: myers
mutable description: Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The design of this part had no special considerations
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pmom
sequence: https://synbiohub.org/public/igem/BBa_K427004_sequence/1

Pmom
type: SequenceAnnotation
persistent identity: annotation2092368
display id: annotation2092368
version: 1
top level: BBa_K427004
location: https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
type: Range
persistent identity: range2092368
display id: range2092368
version: 1
top level: BBa_K427004
direction: direction/forward
start: 1
end: 79
orientation: inline

BBa_K427001
type: ComponentDefinition
persistent identity: BBa K427001
display id: BBa_K427001
version: 1
was generated by: iGEM to SBOL conversion
description: C protein of the Mu bacteriophage
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: Synthetized from the reported sequence of C protein from Mu bacteriophage. It was optimized for its expression in E. coli.
owned by: james
owned by: myers
mutable description: C is a protein of the Mu bacteriophage. It is typically transcribed by the middle promoter of the phage (Pm) and it activates the four late promoters Plys, Pi, Pp and Pmom. Since it is the activator of the late it can be used alongside them to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.
discontinued: false
dominant: false
experience: experience/None
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was optimized for its production on E. coli and the ribosome binding site was added at the beginning of the sequence.
star: false
creator: Jan Marte Contreras Ortiz
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: MuC
sequence: https://synbiohub.org/public/igem/BBa_K427001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2092369
display id: annotation2092369
version: 1
top level: BBa_K427001
location: https://synbiohub.org/public/igem/BBa_K427001/annotation2092369/range2092369/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K427001/annotation2092369/range2092369/1
type: Range
persistent identity: range2092369
display id: range2092369
version: 1
top level: BBa_K427001
direction: direction/forward
start: 1
end: 12
orientation: inline

MuC
type: SequenceAnnotation
persistent identity: annotation2092370
display id: annotation2092370
version: 1
top level: BBa_K427001
location: https://synbiohub.org/public/igem/BBa_K427001/annotation2092370/range2092370/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K427001/annotation2092370/range2092370/1
type: Range
persistent identity: range2092370
display id: range2092370
version: 1
top level: BBa_K427001
direction: direction/forward
start: 18
end: 438
orientation: inline

pBad Weak
type: ComponentDefinition
persistent identity: BBa K427000
display id: BBa_K427000
version: 1
was generated by: iGEM to SBOL conversion
description: PBad Weak Reverse
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:12:29Z
mutable provenance: It was synthesized from the sequence created by British Columbia team in 2009
owned by: james
owned by: myers
mutable description: PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _544_
m user id: 0
owner id: 6605
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We only had to create the reverse complement of the sequence that was already on the registry
star: false
creator: Tatiana J. N????ez Elizondo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PBad Weak Reverse
sequence: https://synbiohub.org/public/igem/BBa_K427000_sequence/1

PBad Weak Reverse
type: SequenceAnnotation
persistent identity: annotation2090357
display id: annotation2090357
version: 1
top level: pBad Weak
location: https://synbiohub.org/public/igem/BBa_K427000/annotation2090357/range2090357/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K427000/annotation2090357/range2090357/1
type: Range
persistent identity: range2090357
display id: range2090357
version: 1
top level: pBad Weak
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_J44002
type: ComponentDefinition
persistent identity: BBa J44002
display id: BBa_J44002
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD reverse
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligonucleotides.
owned by: james
owned by: myers
mutable description: This is the pBAD promoter (BBa_I13453) in the opposite orientation. It can be used to drive transcription in the direction of suffix to prefix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: true
creator: Brad Ogden
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J44002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2002776
display id: annotation2002776
version: 1
top level: BBa_J44002
location: https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
type: Range
persistent identity: range2002776
display id: range2002776
version: 1
top level: BBa_J44002
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
top level: BBa_I746361
direction: direction/forward
start: 1
end: 92
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06702
display id: BBa_J06702
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry, bacterial with RBS and forward terminator
created: 2005-07-24T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Yves Wang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0010
component: BBa_J06504
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06702_sequence/1

BBa_B0034
type: Component
persistent identity: component1596419
display id: component1596419
version: 1
top level: mCherry
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1596432
display id: component1596432
version: 1
top level: mCherry
definition: BBa_B0010
access: public

BBa_J06504
type: Component
persistent identity: component1596427
display id: component1596427
version: 1
top level: mCherry
definition: mCherry
access: public

BBa_B0012
type: Component
persistent identity: component1596442
display id: component1596442
version: 1
top level: mCherry
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1596419
display id: annotation1596419
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596419/range1596419/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06702/annotation1596419/range1596419/1
type: Range
persistent identity: range1596419
display id: range1596419
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1596427
display id: annotation1596427
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596427/range1596427/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J06702/annotation1596427/range1596427/1
type: Range
persistent identity: range1596427
display id: range1596427
version: 1
top level: mCherry
direction: direction/forward
start: 19
end: 732
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596432
display id: annotation1596432
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596432/range1596432/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06702/annotation1596432/range1596432/1
type: Range
persistent identity: range1596432
display id: range1596432
version: 1
top level: mCherry
direction: direction/forward
start: 741
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596442
display id: annotation1596442
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596442/range1596442/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06702/annotation1596442/range1596442/1
type: Range
persistent identity: range1596442
display id: range1596442
version: 1
top level: mCherry
direction: direction/forward
start: 829
end: 869
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD weak
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: Site-directed mutagenesis on <partinfo>I13453</partinfo> with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker version of wild type pBAD (<partinfo>I13453</partinfo>).
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
top level: BBa_K206001
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
top level: BBa_K206001
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
top level: BBa_K206001
direction: direction/forward
start: 61
end: 78
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: phiR73 delta
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
top level: BBa_I746352
direction: direction/forward
start: 1
end: 12
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 264
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 22
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
top level: BBa_I746352
direction: direction/forward
start: 102
end: 102
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

https://synbiohub.org/public/igem/BBa_K427009_sequence/1
type: Sequence
persistent identity: BBa K427009 sequence
display id: BBa_K427009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgactgaagacctgtttggtgatctgcaagacgataccattctggcacacctggataatcctgccgaggacacaagccgttttccggcgctgctggctgaactgaacgatctgctgcgtggtgagctgtctcgcctgggagttgaccctgcccattctctggagattgtcgtggccatttgtaaacatctgggcggaggccaagtttatattccacgtggacaggcactggacagtctgattcgtgacctgcgcatttggaacgatttcaacgggcgtaatgtgagtgaactgactacccgctatggagtgacttttaacaccgtgtataaagccattcgccgtatgcgtcgtctgaaatatcgccagtatcaaccgagcctgctgtactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagaggctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaaagctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagacattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgattcgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctgatcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccctgtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctgtattgccagtttaatggccgtaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0430_sequence/1
type: Sequence
persistent identity: BBa E0430 sequence
display id: BBa_E0430_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427002_sequence/1
type: Sequence
persistent identity: BBa K427002 sequence
display id: BBa_K427002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactgaagacctgtttggtgatctgcaagacgataccattctggcacacctggataatcctgccgaggacacaagccgttttccggcgctgctggctgaactgaacgatctgctgcgtggtgagctgtctcgcctgggagttgaccctgcccattctctggagattgtcgtggccatttgtaaacatctgggcggaggccaagtttatattccacgtggacaggcactggacagtctgattcgtgacctgcgcatttggaacgatttcaacgggcgtaatgtgagtgaactgactacccgctatggagtgacttttaacaccgtgtataaagccattcgccgtatgcgtcgtctgaaatatcgccagtatcaaccgagcctgctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427003_sequence/1
type: Sequence
persistent identity: BBa K427003 sequence
display id: BBa_K427003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttctgtaaacagtaaagccggttaatccggctttttttacgtcctcaatatcctgtgatgaataaccgtac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427004_sequence/1
type: Sequence
persistent identity: BBa K427004 sequence
display id: BBa_K427004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtaatacagatcgattatgccccaataaccacactcaacccatgatgttttttaagatagtggcgaattgatgcaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427001_sequence/1
type: Sequence
persistent identity: BBa K427001 sequence
display id: BBa_K427001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaacatgacctgtttgagcatgatccggcgattcgtcagctgattggccatatcgacaacattccggcacctgaactggaaagtcgctggcctcgtagcgtggttgatctgatcgatgttctggagaacgaactgaaacgccaaaatgtgtctaacccacgtgagctggctcgtaaacaagcagttgccctgtcttgcttcctgggtggacgtcaattctatatcccgtgtggcgacacgatcctgacagcactgcgtgatgatctgctgtattgccagtttaatggccgtaacatggaagaactgcgccgtcaatatcgtctgtctcagccacagatttatcaaatcattgctcgccagcgtaaactgcatacacgtcgccatcaacctgacctgttctctccggaaacaccgaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K427000_sequence/1
type: Sequence
persistent identity: BBa K427000 sequence
display id: BBa_K427000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaaagctatggcatagcaaagtgtgacgccgtgcaaataatcaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44002_sequence/1
type: Sequence
persistent identity: BBa J44002 sequence
display id: BBa_J44002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746361_sequence/1
type: Sequence
persistent identity: BBa I746361 sequence
display id: BBa_I746361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06702_sequence/1
type: Sequence
persistent identity: BBa J06702 sequence
display id: BBa_J06702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206001_sequence/1
type: Sequence
persistent identity: BBa K206001 sequence
display id: BBa_K206001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746352_sequence/1
type: Sequence
persistent identity: BBa I746352 sequence
display id: BBa_I746352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z