LOCUS       BBa_K429000             1785 bp    DNA     linear       23-Dec-2024
DEFINITION  Promoter Testing Construct
ACCESSION   BBa_K429000
VERSION     BBa_K429000.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             929..1648
                     /label=GFP protein
     CDS             929..1648
                     /label=BBa_E0040
     stem_loop       1668..1711
                     /label=stem_loop
     misc_feature    1657..1736
                     /label=BBa_B0010
     terminator      1657..1736
                     /label=BBa_B0010
     stem_loop       1752..1771
                     /label=T7 TE
     misc_feature    1778..1778
                     /label=stop
     polyA_site      1772..1785
                     /label=polya
     misc_feature    1745..1785
                     /label=BBa_B0012
     terminator      1745..1785
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt
      961 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     1021 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     1081 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
     1141 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     1201 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
     1261 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     1321 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     1381 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
     1441 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
     1501 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
     1561 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
     1621 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg
     1681 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1741 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//