LOCUS       BBa_K429001             1870 bp    DNA     linear       26-Nov-2024
DEFINITION  Promoter Testing Construct
ACCESSION   BBa_K429001
VERSION     BBa_K429001.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..793
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     CDS             1014..1733
                     /label=GFP protein
     CDS             1014..1733
                     /label=BBa_E0040
     stem_loop       1753..1796
                     /label=stem_loop
     misc_feature    1742..1821
                     /label=BBa_B0010
     terminator      1742..1821
                     /label=BBa_B0010
     stem_loop       1837..1856
                     /label=T7 TE
     misc_feature    1863..1863
                     /label=stop
     polyA_site      1857..1870
                     /label=polya
     misc_feature    1830..1870
                     /label=BBa_B0012
     terminator      1830..1870
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgcgta
     1021 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1081 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1141 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1201 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1261 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1321 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1381 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1441 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1501 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1561 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1621 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1681 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1741 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1801 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1861 tgcgtttata
//