BBa_K431008
1
BBa_K431008
pTEF ( Translation elongation factor promoter)
2010-09-17T11:00:00Z
2015-05-08T01:12:29Z
This promoter sequence was isolated from yeast Pichia pastoris.
Appl Microbiol Biotechnol. 2007 Mar;74(3):601-8. Epub 2006 Nov 24.
GenBank no. EF014948 http://www.ncbi.nlm.nih.gov/nuccore/116293730
According to research done on this part it has been determined that when comparing glyceraldehydes-3-phosphate dehydrogenase promoter (pGAP) with translational elongation factor promoter (pTEF), pTEF has similar or even greater expression levels of recombinant protein. It was also determined that pTEF does not require an inducer and it can be used in the mass production of heterologuous proteins.
false
false
_570_
0
6828
9
Not in stock
false
In order to determine the appropriate assembly standard that the part can be used with it was necessary to check the sequence for each of the restriction enzymes.
false
Angie Calderon
annotation2080567
1
TEF promoter
range2080567
1
1
424
BBa_K431008_sequence
1
ataactgtcgcctcttttatctgccgcactgcatgaggtgtccccttagtgggaaagagtactgagccaaccctggaggacagcaagggaaaaatacctacaacttgcttcataatggtcgtaaaaacaatccttgtcggatataagtgttgtagactgtcccttatcctctgcgatgttcttcctctcaaagtttgcgatttctctctatcagaattgccatcaagagactcaggactaatttcgcagtcccacacgcactcgtacatgattggctgaaatttccctaaagaatttttttttcacgaaaattttttttttacacaagattttcagcagatataaaatggagagcaggacctccgctgtgactcttcttttttttcttttattctcactacatacattttagttattcgccaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z