BBa_K431010
1
BBa_K431010
Formaldehyde Dehydrogenase (FLD1) from ''Pichia pastoris''
2010-09-20T11:00:00Z
2015-05-08T01:12:29Z
''Pichia pastoris'' genome
Formaldehyde Dehydrogenase (FLD1) from ''Pichia pastoris''
Formaldehyde is a convergent intermediate for utilization of both methanol as a single carbon source and methylamine as a single nitrogen source in ''Pichia pastoris''. Formaldehyde Dehydrogenase is responsible for the oxidation of formaldehyde into formate and is required for cell survival when grown on either methanol or methylamine. For this reason FLD1 has been developed into an alternative marker gene for DNA transformations in fld1- ''P. pastoris'' strains.
It has also been shown that cultures with multiple copies of their expression cassette can be obtained by selecting for organisms with increased formaldehyde resistance. This is a similar concept to that used with increased antibiotic resistance but does not pose a biohazard risk. It also avoids multiple transformations, each of which requires a different marker gene.
Users can therefore use this part for selectivity of transformed cells and to obtain high copies of their desired expression cassette.
false
false
_570_
0
6820
9
Not in stock
false
.
false
David Joseph Sexton Jr.
BBa_K431010_sequence
1
atgtctaccgaaggtcaagtaagttcaatcaaagtaattgtttgggagggaagaagattgttttattgcgaacctttcaatatcttacccgactaaataaccattacagtgaattttttactaactatatagatcatcaaatgtaaggcagctgttgcctgggaggcaggaaaggatctctctattgaggagattgaggttcttcctccaagagcccatgaagttagagtgaaagtggaattcactggtgtatgccacactgatgcttacacgctttctggtgcagatgcagagggaagtttccctgttgtgttcggccatgaaggtgctggtgttgtcgagtcagttggagaaggtgttgagtccgtgaaggttggggattctgtagtgcttctgtacactcctgagtgcagagagtgcaagttctgtctgtctggtaagacgaacctctgtggtaaaatcagagccacccagggtaaaggtttgttaccagacgggacttctcgtttccgttgtaagggcaaggatttgtttcactatatgggatgttcttccttttctcaatacactgtggtggctgacatctcagtggttaaagtccaagacgaagctcctaaggacaagacatgtctgttgggttgtggtgttaccacagggtacggtgctgctatcaacactgctaagatctctaagggtgacaagatcggtgtgtttggtgctggatgtattggattatctgtcatccaaggtgcagtttccaaaggtgcaagcgagattattgtaattgacatcaatgattcaaagaaggcatgggcggaccaatttggtgcaactaagtttgtcaatcctacaaccttaccagaaggtaccaatattgttgactacttgattgatatcactgacggaggctttgactataccttcgactgtaccggtaatgttcaagtaatgagaaatgcacttgaatcttgccacaagggttggggtgagtcgatcatcatcggtgtcgctgctgctggtaaagaaatctctacccgtcctttccagttggttactggcagagtctggagaggatgcgcctttggaggtatcaagggacgtactcaaatgccatctttggttcaggactatcttgatggtaagattaaagttgacgagtttatcacacacagacatgacctggacaacatcaacaaagcatttcatgacatgcatgctggaaactgtattcgtgctgtgattactatgcactaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z