LOCUS       BBa_K496004             2730 bp    DNA     linear       16-Jun-2024
DEFINITION  Reporter of Salmonella secretion signal with NLS, GFP, etc
ACCESSION   BBa_K496004
VERSION     BBa_K496004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1210
                     /label=BBa_I0500 (Arabinose Promoter)
     misc_feature    1218..1804
                     /label=BBa_K496003 (RBS and Slrp signal)
     misc_feature    1811..1831
                     /label=BBa_J63008 (NLS)
     misc_feature    1832..1852
                     /label=BBa_J63008 (NLS)
     misc_feature    1853..1873
                     /label=BBa_J63008 (NLS)
     CDS             1874..2593
                     /label=BBa_E0040 (GFP)
     misc_feature    2602..2730
                     /label=BBa_B0015   (dT)
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagaaaa gaggagaaaa tatgtttaat attactaata tacaatctac
     1261 ggcaaggcat caaagtatta gcaatgaggc ctcaacagag gtgcctttaa aagaagagat
     1321 atggaataaa ataagtgcct ttttctcttc agaacatcag gttgaagcac aaaactgcat
     1381 cgcttatctt tgtcatccac ctgaaaccgc ctcgccagaa gagatcaaaa gcaagtttga
     1441 atgtttaagg atgttagctt tcccggcgta tgcggataat attcagtata gtagaggagg
     1501 ggcagaccaa tactgtattt tgagtgaaaa tagtcaggaa attctgtcta tagtttttaa
     1561 tacagagggc tataccgttg agggaggggg aaagtcagtc acctataccc gtgtgacaga
     1621 aagcgagcag gcgagtagcg cttccggctc caaagatgct gtgaattatg agttaatctg
     1681 gtctgagtgg gtaaaagagg cgccagcgaa agaggcagca aatcgtgaag aagccgtaca
     1741 acggatgcgt gactgcctga aaaataataa gacggaactt cgtctgaaaa tattaggact
     1801 taccactaga cccaagaaaa agcgcaaggt acccaagaaa aagcgcaagg tacccaagaa
     1861 aaagcgcaag gtaatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1921 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1981 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     2041 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     2101 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     2161 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     2221 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     2281 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     2341 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     2401 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     2461 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     2521 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     2581 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2641 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2701 caccttcggg tgggcctttc tgcgtttata
//