LOCUS BBa_K498000 3154 bp DNA linear 22-Nov-2024 DEFINITION T9002 - LacZ ACCESSION BBa_K498000 VERSION BBa_K498000.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 RBS 1070..1082 /label=RBS-3\Weak RBS 1076..1079 /label=RBS misc_feature 1070..1082 /label=BBa_B0032 RBS 1070..1082 /label=BBa_B0032 CDS 1089..1808 /label=GFP protein CDS 1089..1808 /label=BBa_E0040 stem_loop 1828..1871 /label=stem_loop misc_feature 1817..1896 /label=BBa_B0010 terminator 1817..1896 /label=BBa_B0010 stem_loop 1912..1931 /label=T7 TE misc_feature 1938..1938 /label=stop polyA_site 1932..1945 /label=polya misc_feature 1905..1945 /label=BBa_B0012 terminator 1905..1945 /label=BBa_B0012 misc_feature 1..1945 /label=BBa_T9002 misc_feature 1956..1961 /label=BglII misc_feature 1965..1970 /label=BamHI misc_feature 1954..1972 /label=BBa_I732007 misc_feature 1985..1988 /label=conserved RBS 1981..1992 /label=BBa_B0034 CDS 1999..2232 /label=lacZ misc_feature 1999..2001 /label=START misc_feature 2227..2232 /label=STOP CDS 1999..2232 /label=BBa_I732006 misc_feature 2245..2248 /label=conserved RBS 2241..2252 /label=BBa_B0034 misc_feature 2259..3017 /label=BBa_E0044 stem_loop 3037..3080 /label=stem_loop misc_feature 3026..3105 /label=BBa_B0010 terminator 3026..3105 /label=BBa_B0010 stem_loop 3121..3140 /label=T7 TE misc_feature 3147..3147 /label=stop polyA_site 3141..3154 /label=polya misc_feature 3114..3154 /label=BBa_B0012 terminator 3114..3154 /label=BBa_B0012 misc_feature 1954..3154 /label=BBa_I732094 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa 1081 agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 1801 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1861 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1921 tcgggtgggc ctttctgcgt ttatatacta gaggaagatc ttccggatcc ggtactagag 1981 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt 2041 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 2101 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 2161 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 2221 ctggagtaat aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt 2281 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt 2341 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 2401 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg 2461 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca 2521 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga 2581 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta 2641 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 2701 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta 2761 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa 2821 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt 2881 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg 2941 ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac gaaaactacg 3001 ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3061 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3121 ggctcacctt cgggtgggcc tttctgcgtt tata //