##gff-version 3
##sequence-region BBa_K498000 1 3154
BBa_K498000	.	engineered_region	1954	3154	.	+	0	ID=BBa_I732094;Name=BBa_I732094
BBa_K498000	.	terminator	3114	3154	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732094
BBa_K498000	.	engineered_region	3114	3154	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K498000	.	stop_codon	3147	3147	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K498000	.	stem_loop	3121	3140	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K498000	.	polyA_site	3141	3154	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K498000	.	ribosome_entry_site	1981	1992	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K498000	.	conserved_region	1985	1988	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K498000	.	CDS	1999	2232	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732094
BBa_K498000	.	start_codon	1999	2001	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K498000	.	stop_codon	2227	2232	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K498000	.	CDS	1999	2232	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K498000	.	engineered_region	2259	3017	.	+	0	ID=BBa_E0044;Name=BBa_E0044;Parent=BBa_I732094
BBa_K498000	.	ribosome_entry_site	2241	2252	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K498000	.	conserved_region	2245	2248	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K498000	.	terminator	3026	3105	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732094
BBa_K498000	.	stem_loop	3037	3080	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K498000	.	engineered_region	3026	3105	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K498000	.	sequence_feature	1954	1972	.	+	0	ID=BBa_I732007;Name=BBa_I732007;Parent=BBa_I732094
BBa_K498000	.	sequence_feature	1965	1970	.	+	0	ID=annotation1936908;Name=BamHI;Parent=BBa_I732007
BBa_K498000	.	sequence_feature	1956	1961	.	+	0	ID=annotation1936907;Name=BglII;Parent=BBa_I732007
BBa_K498000	.	engineered_region	1	1945	.	+	0	ID=BBa_T9002;Name=BBa_T9002
BBa_K498000	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K498000	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K498000	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K498000	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K498000	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K498000	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_T9002
BBa_K498000	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K498000	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K498000	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K498000	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K498000	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K498000	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K498000	.	terminator	1817	1896	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K498000	.	stem_loop	1828	1871	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K498000	.	engineered_region	1817	1896	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K498000	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K498000	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K498000	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K498000	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_T9002
BBa_K498000	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K498000	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_T9002
BBa_K498000	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K498000	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K498000	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K498000	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K498000	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K498000	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_T9002
BBa_K498000	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K498000	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K498000	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K498000	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K498000	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K498000	.	ribosome_entry_site	1070	1082	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_T9002
BBa_K498000	.	ribosome_entry_site	1076	1079	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K498000	.	engineered_region	1070	1082	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K498000	.	ribosome_entry_site	1070	1082	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K498000	.	terminator	1905	1945	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K498000	.	engineered_region	1905	1945	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K498000	.	stop_codon	1938	1938	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K498000	.	stem_loop	1912	1931	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K498000	.	polyA_site	1932	1945	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K498000	.	CDS	1089	1808	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_T9002
BBa_K498000	.	CDS	1089	1808	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K498000
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggaagatcttccggatccggtac
tagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaa
ccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcacc
gatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccgg
aaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgct
gctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata