LOCUS BBa_K510002 4659 bp DNA linear 08-Nov-2024 DEFINITION pUC18SfiI-miniTn7BB-Km ACCESSION BBa_K510002 VERSION BBa_K510002.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix misc_feature 4638..4658 /label=Prefix misc_feature 82..347 /label=Tn7-L end misc_feature 3014..3213 /label=Tn7-R end misc_feature 348..360 /label=SfiI restriction site CDS 966..1826 /label=Ampicillin resistance (bla) misc_feature 1965..2644 /label=ColEI (pUC18) Replication origin misc_feature 3002..3014 /label=SfiI restriction site misc_feature 3329..3376 /label=Flipase recombination sequence (FRT) misc_feature 4467..4514 /label=Flipase recombination sequence (FRT) CDS 3467..4285 /label=Kanamycin resistance ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg 2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 3001 tggccgccta ggcctgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 3061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 3121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 3181 gcaaattgcc cgtcgtatta aagaggggcg tggggttcgc ttgtcacact ggctcacctt 3241 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat 3301 ccggcttttt tattatttgc tagatcttga agtacctatt ccgaagttcc tattctctaa 3361 aaagtatagg aacttcagag gcatgcccat ggattggggc tcactcaaag gcggtaatca 3421 gataaaaaaa atccttagct ttcgctaagg atgatttctg ctagtattat tagaaaaact 3481 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt 3541 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccaa agaatggcaa 3601 ggtcctggta acggtctgcg attccgaccc gtccaacatc aatacaacct attaatttcc 3661 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg 3721 agaatggcaa gagcttgtgc atttctttcc agacttgttc aacaggccag ccattacgct 3781 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat gcgtgattgc gcctgagcaa 3841 gacgaaatac acgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgtaaccggc 3901 gcaggaacac ggccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata 3961 cctggaaggc tgttttccca ggaatcgcgg tggtgagtaa ccacgcatca tcaggagtac 4021 ggataaaatg cttgatggtc gggagaggca taaactccgt cagccagttg agacggacca 4081 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg 4141 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag 4201 cccatttata cccatataaa tcagcgtcca tgttggagtt taagcgcgga cgggagcaag 4261 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca 4321 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag 4381 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagctctagt 4441 agttacattg tcgaccatgg gcatgcgaag tacctattcc gaagttccta ttctctaaaa 4501 agtataggaa cttcagagct gccacctgac gtctaagaaa ccattattat catgacatta 4561 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc 4621 gctaaggatg atttctggaa ttcgcggccg cttctagag //