##gff-version 3
##sequence-region BBa_K510013 2 4661
BBa_K510013	.	sequence_feature	3548	3553	.	+	0	ID=annotation2132797;Name=NcoI restriction site
BBa_K510013	.	engineered_region	4640	4661	.	+	0	ID=annotation2133000;Name=Prefix
BBa_K510013	.	primer_binding_site	157	176	.	+	0	ID=annotation2132735;Name=VR (BBa_G00101) primer binding site
BBa_K510013	.	sequence_feature	646	905	.	+	0	ID=annotation2132738;Name=oriT
BBa_K510013	.	sequence_feature	3163	3175	.	+	0	ID=annotation2132741;Name=SfiI restriction site
BBa_K510013	.	CDS	1714	2574	.	+	0	ID=annotation2132740;Name=Ampicillin resistance (bla)
BBa_K510013	.	engineered_region	3385	3479	.	+	0	ID=annotation2132733;Name=double terminator (BBa_B0014)
BBa_K510013	.	CDS	3696	4355	.	+	0	ID=annotation2132796;Name=Chloramphenicol resistance
BBa_K510013	.	sequence_feature	4457	4462	.	+	0	ID=annotation2132795;Name=NcoI restriction site
BBa_K510013	.	stem_loop	22	93	.	+	0	ID=annotation2132734;Name=E. coli his operon terminator
BBa_K510013	.	sequence_feature	3490	3537	.	+	0	ID=annotation2132800;Name=Flipase recombination sequence (FRT)
BBa_K510013	.	stem_loop	3577	3683	.	+	0	ID=annotation2132872;Name=T0 Terminator
BBa_K510013	.	sequence_feature	4469	4516	.	+	0	ID=annotation2132799;Name=Flipase recombination sequence (FRT)
BBa_K510013	.	sequence_feature	3176	3374	.	+	0	ID=annotation2132742;Name=Tn7-R end
BBa_K510013	.	stem_loop	4600	4632	.	+	0	ID=annotation2132825;Name=Terminator
BBa_K510013	.	sequence_feature	182	347	.	+	0	ID=annotation2132736;Name=Tn7-L end
BBa_K510013	.	sequence_feature	929	1310	.	+	0	ID=annotation2132739;Name=R6K replication origin
BBa_K510013	.	sequence_feature	4463	4468	.	+	0	ID=annotation2132794;Name=SphI restriction site
BBa_K510013	.	primer_binding_site	4522	4541	.	+	0	ID=annotation2132826;Name=VF2 (G00100) primer binding site
BBa_K510013	.	sequence_feature	348	369	.	+	0	ID=annotation2132737;Name=SfiI restriction site
BBa_K510013	.	engineered_region	2	21	.	+	0	ID=annotation2132743;Name=Suffix
BBa_K510013	.	sequence_feature	3542	3547	.	+	0	ID=annotation2132798;Name=SphI restriction site
>BBa_K510013
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggaa
cttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccag
atggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgcca
ctcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatc
gccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatat
tggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacccttt
agggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtgg
tattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatca
ccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccgg
ataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacat
tgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgattt
ttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcatt
atggtgaaagttggaacctcttacgtgcccgaccatgggcatgcgaagtacctattccgaagttcctattctctaaaaa
gtataggaacttcagagctgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtat
cacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag