LOCUS BBa_K510014 4820 bp DNA linear 05-Nov-2024 DEFINITION pUC18R6KT-miniTn7BB-Km ACCESSION BBa_K510014 VERSION BBa_K510014.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix misc_feature 182..347 /label=Tn7-L end misc_feature 3163..3175 /label=SfiI restriction site misc_feature 3385..3479 /label=double terminator (BBa_B0014) misc_feature 3176..3374 /label=Tn7-R end primer_bind 157..176 /label=VR (BBa_G00101) primer binding site CDS 1714..2574 /label=Ampicillin resistance (bla) misc_feature 929..1310 /label=R6K replication origin misc_feature 646..905 /label=oriT misc_feature 348..369 /label=SfiI restriction site stem_loop 22..93 /label=E. coli his operon terminator misc_feature 3542..3547 /label=SphI restriction site misc_feature 4628..4675 /label=Flipase recombination sequence (FRT) misc_feature 4616..4627 /label=SfiI restriction site CDS 3628..4446 /label=Kanamycin resistance stem_loop 3580..3620 /label=Termiantor misc_feature 3548..3553 /label=NcoI restriction site misc_feature 3490..3537 /label=Flipase recombination sequence (FRT) primer_bind 4681..4700 /label=VF2 (G00100) primer binding site misc_feature 4798..4820 /label=Prefix stem_loop 4759..4791 /label=Terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgttaag gtatactttc 661 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt 721 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg 781 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac 841 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat 901 aagctctgat aacgcaggaa agaacatggg cagttcaacc tgttgatagt acgtactaag 961 ctctcatgtt tcacgtacta agctctcaag tttaacgtac taagctctca tgtttaacga 1021 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt 1081 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag 1141 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta 1201 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc 1261 tctcaaagca attttgagtg acacaggaac acttaacggc tgacaaccgg ctggtaacag 1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 1741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 1801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 1861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 1921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 1981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 2581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 2641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 2701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 2761 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2821 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2881 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2941 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 3001 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 3061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 3121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccgcct aggcctgtgg 3181 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 3241 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 3301 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 3361 aaagaggggc gtggggttcg cttgtcacac tggctcacct tcgggtgggc ctttctgcgt 3421 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttg 3481 ctagatcttg aagtacctat tccgaagttc ctattctcta aaaagtatag gaacttcaga 3541 ggcatgccca tggattgggg ctcactcaaa ggcggtaatc agataaaaaa aatccttagc 3601 tttcgctaag gatgatttct gctagtatta ttagaaaaac tcatcgagca tcaaatgaaa 3661 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa 3721 tgaaggagaa aactcaccga ggcagttcca aagaatggca aggtcctggt aacggtctgc 3781 gattccgacc cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt 3841 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca agagcttgtg 3901 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc 3961 atcaaccaaa ccgttattca tgcgtgattg cgcctgagca agacgaaata cacgatcgct 4021 gttaaaagga caattacaaa caggaatcga atgtaaccgg cgcaggaaca cggccagcgc 4081 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaagg ctgttttccc 4141 aggaatcgcg gtggtgagta accacgcatc atcaggagta cggataaaat gcttgatggt 4201 cgggagaggc ataaactccg tcagccagtt gagacggacc atctcatctg taacatcatt 4261 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa 4321 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa 4381 atcagcgtcc atgttggagt ttaagcgcgg acgggagcaa gacgtttccc gttgaatatg 4441 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga 4501 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg 4561 aataaatcga acttttgctg agttgaagga tcagctctag tagttacatt gtcgaccatg 4621 ggcatgcgaa gtacctattc cgaagttcct attctctaaa aagtatagga acttcagagc 4681 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 4741 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga 4801 attcgcggcc gcttctagag //