##gff-version 3
##sequence-region BBa_K510014 2 4820
BBa_K510014	.	stem_loop	22	93	.	+	0	ID=annotation2133011;Name=E. coli his operon terminator
BBa_K510014	.	sequence_feature	929	1310	.	+	0	ID=annotation2133008;Name=R6K replication origin
BBa_K510014	.	sequence_feature	3176	3374	.	+	0	ID=annotation2133005;Name=Tn7-R end
BBa_K510014	.	stem_loop	3580	3620	.	+	0	ID=annotation2133016;Name=Termiantor
BBa_K510014	.	sequence_feature	3542	3547	.	+	0	ID=annotation2133012;Name=SphI restriction site
BBa_K510014	.	engineered_region	2	21	.	+	0	ID=annotation2133001;Name=Suffix
BBa_K510014	.	primer_binding_site	157	176	.	+	0	ID=annotation2133006;Name=VR (BBa_G00101) primer binding site
BBa_K510014	.	sequence_feature	646	905	.	+	0	ID=annotation2133009;Name=oriT
BBa_K510014	.	sequence_feature	182	347	.	+	0	ID=annotation2133002;Name=Tn7-L end
BBa_K510014	.	stem_loop	4759	4791	.	+	0	ID=annotation2133096;Name=Terminator
BBa_K510014	.	sequence_feature	3548	3553	.	+	0	ID=annotation2133017;Name=NcoI restriction site
BBa_K510014	.	primer_binding_site	4681	4700	.	+	0	ID=annotation2133094;Name=VF2 (G00100) primer binding site
BBa_K510014	.	CDS	3628	4446	.	+	0	ID=annotation2133015;Name=Kanamycin resistance
BBa_K510014	.	sequence_feature	4616	4627	.	+	0	ID=annotation2133014;Name=SfiI restriction site
BBa_K510014	.	sequence_feature	3163	3175	.	+	0	ID=annotation2133003;Name=SfiI restriction site
BBa_K510014	.	engineered_region	3385	3479	.	+	0	ID=annotation2133004;Name=double terminator (BBa_B0014)
BBa_K510014	.	sequence_feature	348	369	.	+	0	ID=annotation2133010;Name=SfiI restriction site
BBa_K510014	.	CDS	1714	2574	.	+	0	ID=annotation2133007;Name=Ampicillin resistance (bla)
BBa_K510014	.	engineered_region	4798	4820	.	+	0	ID=annotation2133095;Name=Prefix
BBa_K510014	.	sequence_feature	4628	4675	.	+	0	ID=annotation2133013;Name=Flipase recombination sequence (FRT)
BBa_K510014	.	sequence_feature	3490	3537	.	+	0	ID=annotation2133018;Name=Flipase recombination sequence (FRT)
>BBa_K510014
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggat
tggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagtattattag
aaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtt
tctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccg
tccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgact
gaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaa
aatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaagg
acaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcagga
tattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataa
aatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaac
gctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgc
ccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacg
tttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatat
atttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgag
ttgaaggatcagctctagtagttacattgtcgaccatgggcatgcgaagtacctattccgaagttcctattctctaaaa
agtataggaacttcagagctgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgta
tcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag
g