BBa_K510015
type: ComponentDefinition
persistent identity: BBa K510015
display id: BBa_K510015
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm-RBS+RFP
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: Cloning of BBa_K093005 in the BCS of BBa_K510012.
owned by: james
owned by: myers
mutable description: The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a RBS+RFP cassette (BBa_K093005) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism using the transposase machinery of the Tn7 transposon.
discontinued: false
dominant: false
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm-RBS+RFP by EcoRI and PstI clonning.
star: false
creator: David Caballero
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K093005
component: BBa_K510012
sequence annotation: BBa_K510012
sequence annotation: BBa_K093005
sequence: https://synbiohub.org/public/igem/BBa_K510015_sequence/1

BBa_K093005
type: Component
persistent identity: component2247783
display id: component2247783
version: 1
top level: BBa_K510015
definition: RBS-RFP
access: public

BBa_K510012
type: Component
persistent identity: component2247777
display id: component2247777
version: 1
top level: BBa_K510015
definition: BBa_K510012
access: public

BBa_K510012
type: SequenceAnnotation
persistent identity: annotation2247777
display id: annotation2247777
version: 1
top level: BBa_K510015
location: https://synbiohub.org/public/igem/BBa_K510015/annotation2247777/range2247777/1
component: BBa_K510012

https://synbiohub.org/public/igem/BBa_K510015/annotation2247777/range2247777/1
type: Range
persistent identity: range2247777
display id: range2247777
version: 1
top level: BBa_K510015
direction: direction/reverse
start: 1
end: 4549
orientation: inline

BBa_K093005
type: SequenceAnnotation
persistent identity: annotation2247783
display id: annotation2247783
version: 1
top level: BBa_K510015
location: https://synbiohub.org/public/igem/BBa_K510015/annotation2247783/range2247783/1
component: BBa_K093005

https://synbiohub.org/public/igem/BBa_K510015/annotation2247783/range2247783/1
type: Range
persistent identity: range2247783
display id: range2247783
version: 1
top level: BBa_K510015
direction: direction/reverse
start: 4558
end: 5281
orientation: inline

BBa_K510012
type: ComponentDefinition
persistent identity: BBa K510012
display id: BBa_K510012
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: pUC18R6KT-miniTn7BB-Gm is a vehicle vector for the minitransposon miniTn7BB-Gm. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacteria. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. This plasmid is replicative only in E. coli strains expressing the pir gene thanks to its R6K replication origin. Also it has an origin for transference (oriT) for conjugation purposes. This plasmid can be used to maintain the miniTn7-Gm transposon in E. coli pir+ strains and may be used for transposition into the genomes of any bacteria without the pir gen, in which it is non-replicative. As a BioBrick cloning vector, pUC18R6KT-miniTn7BB-Gm is fully compatible with Assembly Standard 10. The complete transposon is flanked by SfiI restriction sites to facilitate subcloning in other vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: oriT
sequence annotation: R6K replication origin
sequence annotation: Ampicillin resistance (bla)
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restriction site
sequence annotation: Gentamicin resistance
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510012_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2131765
display id: annotation2131765
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
type: Range
persistent identity: range2131765
display id: range2131765
version: 1
top level: BBa_K510012
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2131863
display id: annotation2131863
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
type: Range
persistent identity: range2131863
display id: range2131863
version: 1
top level: BBa_K510012
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131786
display id: annotation2131786
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
type: Range
persistent identity: range2131786
display id: range2131786
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2131766
display id: annotation2131766
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
type: Range
persistent identity: range2131766
display id: range2131766
version: 1
top level: BBa_K510012
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131798
display id: annotation2131798
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
type: Range
persistent identity: range2131798
display id: range2131798
version: 1
top level: BBa_K510012
direction: direction/forward
start: 348
end: 360
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2131797
display id: annotation2131797
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
type: Range
persistent identity: range2131797
display id: range2131797
version: 1
top level: BBa_K510012
direction: direction/forward
start: 646
end: 905
orientation: inline

R6K replication origin
type: SequenceAnnotation
persistent identity: annotation2131796
display id: annotation2131796
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
type: Range
persistent identity: range2131796
display id: range2131796
version: 1
top level: BBa_K510012
direction: direction/forward
start: 929
end: 1310
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2131795
display id: annotation2131795
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
type: Range
persistent identity: range2131795
display id: range2131795
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 1714
end: 2574
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131803
display id: annotation2131803
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
type: Range
persistent identity: range2131803
display id: range2131803
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3163
end: 3175
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2131800
display id: annotation2131800
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
type: Range
persistent identity: range2131800
display id: range2131800
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3176
end: 3374
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2131801
display id: annotation2131801
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
type: Range
persistent identity: range2131801
display id: range2131801
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3385
end: 3479
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131802
display id: annotation2131802
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
type: Range
persistent identity: range2131802
display id: range2131802
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3490
end: 3537
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131806
display id: annotation2131806
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
type: Range
persistent identity: range2131806
display id: range2131806
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3542
end: 3547
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131804
display id: annotation2131804
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
type: Range
persistent identity: range2131804
display id: range2131804
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3548
end: 3553
orientation: inline

Gentamicin resistance
type: SequenceAnnotation
persistent identity: annotation2131805
display id: annotation2131805
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
type: Range
persistent identity: range2131805
display id: range2131805
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 3554
end: 4087
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131807
display id: annotation2131807
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
type: Range
persistent identity: range2131807
display id: range2131807
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4345
end: 4350
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131808
display id: annotation2131808
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
type: Range
persistent identity: range2131808
display id: range2131808
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4351
end: 4355
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131856
display id: annotation2131856
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
type: Range
persistent identity: range2131856
display id: range2131856
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4357
end: 4404
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131841
display id: annotation2131841
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
type: Range
persistent identity: range2131841
display id: range2131841
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4410
end: 4429
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2131866
display id: annotation2131866
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
type: Range
persistent identity: range2131866
display id: range2131866
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4487
end: 4520
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2131839
display id: annotation2131839
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
type: Range
persistent identity: range2131839
display id: range2131839
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4528
end: 4549
orientation: inline

RBS-RFP
type: ComponentDefinition
persistent identity: BBa K093005
display id: BBa_K093005
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: registry plasmids
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.
discontinued: false
dominant: false
experience: experience/Works
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site.
star: true
creator: Kathy Lam, Danielle Nash
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K093005_sequence/1

BBa_B0034
type: Component
persistent identity: component2244022
display id: component2244022
version: 1
top level: RBS-RFP
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2244025
display id: component2244025
version: 1
top level: RBS-RFP
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244022
display id: annotation2244022
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
type: Range
persistent identity: range2244022
display id: range2244022
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244025
display id: annotation2244025
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
type: Range
persistent identity: range2244025
display id: range2244025
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K510015_sequence/1
type: Sequence
persistent identity: BBa K510015 sequence
display id: BBa_K510015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510012_sequence/1
type: Sequence
persistent identity: BBa K510012 sequence
display id: BBa_K510012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093005_sequence/1
type: Sequence
persistent identity: BBa K093005 sequence
display id: BBa_K093005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z