LOCUS BBa_K510017 5446 bp DNA linear 26-Dec-2024 DEFINITION pUC18R6KT-miniTn7BB-Gm-tetR ACCESSION BBa_K510017 VERSION BBa_K510017.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix misc_feature 182..347 /label=Tn7-L end primer_bind 157..176 /label=VR (BBa_G00101) primer binding site CDS 1714..2574 /label=Ampicillin resistance (bla) misc_feature 929..1310 /label=R6K replication origin misc_feature 646..905 /label=oriT misc_feature 348..360 /label=SfiI restriction site misc_feature 3176..3374 /label=Tn7-R end misc_feature 3385..3479 /label=double terminator (BBa_B0014) misc_feature 3490..3537 /label=Flipase recombination sequence (FRT) misc_feature 3163..3175 /label=SfiI restriction site misc_feature 3548..3553 /label=NcoI restriction site CDS 3554..4087 /label=Gentamicin resistance misc_feature 3542..3547 /label=SphI restriction site misc_feature 4345..4350 /label=NcoI restriction site misc_feature 4351..4355 /label=SphI restriction site misc_feature 4528..4549 /label=Prefix primer_bind 4410..4429 /label=VF2 (G00100) primer binding site misc_feature 4357..4404 /label=Flipase recombination sequence (FRT) stem_loop 22..93 /label=E. coli his operon terminator stem_loop 4487..4520 /label=Terminator misc_feature 1..4549 /label=BBa_K510012 misc_feature 4558..4594 /label=P(Lac) IQ promoter 4560..4565 /label=-35 promoter 4583..4588 /label=-10 promoter 4558..4594 /label=BBa_I14032 misc_feature 4607..4610 /label=conserved RBS 4603..4614 /label=BBa_B0034 CDS 4621..5247 /label=tetR CDS 4621..5247 /label=BBa_I732080 stem_loop 5267..5310 /label=stem_loop misc_feature 5256..5335 /label=BBa_B0010 terminator 5256..5335 /label=BBa_B0010 stem_loop 5351..5370 /label=T7 TE misc_feature 5377..5377 /label=stop polyA_site 5371..5384 /label=polya misc_feature 5344..5384 /label=BBa_B0012 terminator 5344..5384 /label=BBa_B0012 misc_feature 5393..5411 /label=TetR 1 misc_feature 5393..5446 /label=BBa_R0040 promoter 5412..5417 /label=-35 misc_feature 5418..5436 /label=TetR 2 promoter 5435..5440 /label=-10 promoter 5393..5446 /label=BBa_R0040 misc_feature 4558..5446 /label=BBa_I732083 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgttaag gtatactttc 661 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt 721 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg 781 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac 841 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat 901 aagctctgat aacgcaggaa agaacatggg cagttcaacc tgttgatagt acgtactaag 961 ctctcatgtt tcacgtacta agctctcaag tttaacgtac taagctctca tgtttaacga 1021 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt 1081 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag 1141 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta 1201 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc 1261 tctcaaagca attttgagtg acacaggaac acttaacggc tgacaaccgg ctggtaacag 1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 1741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 1801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 1861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 1921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 1981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 2581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 2641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 2701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 2761 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2821 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2881 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2941 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 3001 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 3061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 3121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccgcct aggcctgtgg 3181 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 3241 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 3301 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 3361 aaagaggggc gtggggttcg cttgtcacac tggctcacct tcgggtgggc ctttctgcgt 3421 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttg 3481 ctagatcttg aagtacctat tccgaagttc ctattctcta aaaagtatag gaacttcaga 3541 ggcatgccca tggttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc 3601 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt 3661 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg 3721 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac 3781 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt 3841 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt 3901 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag 3961 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac 4021 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc 4081 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc 4141 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc 4201 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata 4261 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaattcgat ccattgctgt 4321 tgacaaaggg aatcagggga tcttccatgg gcatgcgaag tacctattcc gaagttccta 4381 ttctctaaaa agtataggaa cttcagagct gccacctgac gtctaagaaa ccattattat 4441 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc 4501 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt actagagtgg 4561 tgcaaaacct ttcgcggtat ggcatgatag cgcctactag agaaagagga gaaatactag 4621 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc 4681 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca 4741 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta 4801 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt 4861 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat 4921 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta 4981 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt 5041 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca 5101 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa 5161 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa 5221 cttaaatgtg aaagtgggtc ctaataatac tagagccagg catcaaataa aacgaaaggc 5281 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 5341 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc 5401 agtgatagag attgacatcc ctatcagtga tagagatact gagcac //