BBa_K510017
type: ComponentDefinition
persistent identity: BBa K510017
display id: BBa_K510017
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm-tetR
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a tetR/ptetR cassette (BBa_I732083) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The BBa_I732083 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I732083
component: BBa_K510012
sequence annotation: BBa_K510012
sequence annotation: BBa_I732083
sequence: https://synbiohub.org/public/igem/BBa_K510017_sequence/1

BBa_I732083
type: Component
persistent identity: component2134658
display id: component2134658
version: 1
top level: BBa_K510017
definition: BBa_I732083
access: public

BBa_K510012
type: Component
persistent identity: component2134638
display id: component2134638
version: 1
top level: BBa_K510017
definition: BBa_K510012
access: public

BBa_K510012
type: SequenceAnnotation
persistent identity: annotation2134638
display id: annotation2134638
version: 1
top level: BBa_K510017
location: https://synbiohub.org/public/igem/BBa_K510017/annotation2134638/range2134638/1
component: BBa_K510012

https://synbiohub.org/public/igem/BBa_K510017/annotation2134638/range2134638/1
type: Range
persistent identity: range2134638
display id: range2134638
version: 1
top level: BBa_K510017
direction: direction/reverse
start: 1
end: 4549
orientation: inline

BBa_I732083
type: SequenceAnnotation
persistent identity: annotation2134658
display id: annotation2134658
version: 1
top level: BBa_K510017
location: https://synbiohub.org/public/igem/BBa_K510017/annotation2134658/range2134658/1
component: BBa_I732083

https://synbiohub.org/public/igem/BBa_K510017/annotation2134658/range2134658/1
type: Range
persistent identity: range2134658
display id: range2134658
version: 1
top level: BBa_K510017
direction: direction/reverse
start: 4558
end: 5446
orientation: inline

BBa_K510012
type: ComponentDefinition
persistent identity: BBa K510012
display id: BBa_K510012
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: pUC18R6KT-miniTn7BB-Gm is a vehicle vector for the minitransposon miniTn7BB-Gm. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacteria. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. This plasmid is replicative only in E. coli strains expressing the pir gene thanks to its R6K replication origin. Also it has an origin for transference (oriT) for conjugation purposes. This plasmid can be used to maintain the miniTn7-Gm transposon in E. coli pir+ strains and may be used for transposition into the genomes of any bacteria without the pir gen, in which it is non-replicative. As a BioBrick cloning vector, pUC18R6KT-miniTn7BB-Gm is fully compatible with Assembly Standard 10. The complete transposon is flanked by SfiI restriction sites to facilitate subcloning in other vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: oriT
sequence annotation: R6K replication origin
sequence annotation: Ampicillin resistance (bla)
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restriction site
sequence annotation: Gentamicin resistance
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510012_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2131765
display id: annotation2131765
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
type: Range
persistent identity: range2131765
display id: range2131765
version: 1
top level: BBa_K510012
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2131863
display id: annotation2131863
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
type: Range
persistent identity: range2131863
display id: range2131863
version: 1
top level: BBa_K510012
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131786
display id: annotation2131786
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
role: SO:0005850
role: feature/primer binding

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type: Range
persistent identity: range2131786
display id: range2131786
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2131766
display id: annotation2131766
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
type: Range
persistent identity: range2131766
display id: range2131766
version: 1
top level: BBa_K510012
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131798
display id: annotation2131798
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
type: Range
persistent identity: range2131798
display id: range2131798
version: 1
top level: BBa_K510012
direction: direction/forward
start: 348
end: 360
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2131797
display id: annotation2131797
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
type: Range
persistent identity: range2131797
display id: range2131797
version: 1
top level: BBa_K510012
direction: direction/forward
start: 646
end: 905
orientation: inline

R6K replication origin
type: SequenceAnnotation
persistent identity: annotation2131796
display id: annotation2131796
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
type: Range
persistent identity: range2131796
display id: range2131796
version: 1
top level: BBa_K510012
direction: direction/forward
start: 929
end: 1310
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2131795
display id: annotation2131795
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
type: Range
persistent identity: range2131795
display id: range2131795
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 1714
end: 2574
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131803
display id: annotation2131803
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
type: Range
persistent identity: range2131803
display id: range2131803
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3163
end: 3175
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2131800
display id: annotation2131800
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
type: Range
persistent identity: range2131800
display id: range2131800
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3176
end: 3374
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2131801
display id: annotation2131801
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
type: Range
persistent identity: range2131801
display id: range2131801
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3385
end: 3479
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131802
display id: annotation2131802
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
type: Range
persistent identity: range2131802
display id: range2131802
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3490
end: 3537
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131806
display id: annotation2131806
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
type: Range
persistent identity: range2131806
display id: range2131806
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3542
end: 3547
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131804
display id: annotation2131804
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
type: Range
persistent identity: range2131804
display id: range2131804
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3548
end: 3553
orientation: inline

Gentamicin resistance
type: SequenceAnnotation
persistent identity: annotation2131805
display id: annotation2131805
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
type: Range
persistent identity: range2131805
display id: range2131805
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 3554
end: 4087
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131807
display id: annotation2131807
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131807
display id: range2131807
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4345
end: 4350
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131808
display id: annotation2131808
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
type: Range
persistent identity: range2131808
display id: range2131808
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4351
end: 4355
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131856
display id: annotation2131856
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
type: Range
persistent identity: range2131856
display id: range2131856
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4357
end: 4404
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131841
display id: annotation2131841
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
type: Range
persistent identity: range2131841
display id: range2131841
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4410
end: 4429
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2131866
display id: annotation2131866
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
type: Range
persistent identity: range2131866
display id: range2131866
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4487
end: 4520
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2131839
display id: annotation2131839
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
type: Range
persistent identity: range2131839
display id: range2131839
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4528
end: 4549
orientation: inline

BBa_I732083
type: ComponentDefinition
persistent identity: BBa I732083
display id: BBa_I732083
version: 1
was generated by: iGEM to SBOL conversion
description: [aTc] -> PoPS
created: 2007-08-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_I732080
component: BBa_B0034
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_B0034
sequence annotation: BBa_I732080
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I732083_sequence/1

BBa_R0040
type: Component
persistent identity: component1940784
display id: component1940784
version: 1
top level: BBa_I732083
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1940779
display id: component1940779
version: 1
top level: BBa_I732083
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1940777
display id: component1940777
version: 1
top level: BBa_I732083
definition: BBa_B0010
access: public

BBa_I732080
type: Component
persistent identity: component1940776
display id: component1940776
version: 1
top level: BBa_I732083
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component1940774
display id: component1940774
version: 1
top level: BBa_I732083
definition: BBa_B0034
access: public

BBa_I14032
type: Component
persistent identity: component1940772
display id: component1940772
version: 1
top level: BBa_I732083
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1940772
display id: annotation1940772
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940772/range1940772/1
component: BBa_I14032

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type: Range
persistent identity: range1940772
display id: range1940772
version: 1
top level: BBa_I732083
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940774
display id: annotation1940774
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940774/range1940774/1
component: BBa_B0034

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type: Range
persistent identity: range1940774
display id: range1940774
version: 1
top level: BBa_I732083
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_I732080
type: SequenceAnnotation
persistent identity: annotation1940776
display id: annotation1940776
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940776/range1940776/1
component: BBa_I732080

https://synbiohub.org/public/igem/BBa_I732083/annotation1940776/range1940776/1
type: Range
persistent identity: range1940776
display id: range1940776
version: 1
top level: BBa_I732083
direction: direction/reverse
start: 64
end: 690
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1940777
display id: annotation1940777
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940777/range1940777/1
component: BBa_B0010

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type: Range
persistent identity: range1940777
display id: range1940777
version: 1
top level: BBa_I732083
direction: direction/forward
start: 699
end: 778
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1940779
display id: annotation1940779
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940779/range1940779/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732083/annotation1940779/range1940779/1
type: Range
persistent identity: range1940779
display id: range1940779
version: 1
top level: BBa_I732083
direction: direction/forward
start: 787
end: 827
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1940784
display id: annotation1940784
version: 1
top level: BBa_I732083
location: https://synbiohub.org/public/igem/BBa_I732083/annotation1940784/range1940784/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I732083/annotation1940784/range1940784/1
type: Range
persistent identity: range1940784
display id: range1940784
version: 1
top level: BBa_I732083
direction: direction/forward
start: 836
end: 889
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa I732080
display id: BBa_I732080
version: 1
was generated by: iGEM to SBOL conversion
description: Tet repressor (RBS- LVA- TERM-)
created: 2007-08-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_I732080_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation1940756
display id: annotation1940756
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_I732080/annotation1940756/range1940756/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732080/annotation1940756/range1940756/1
type: Range
persistent identity: range1940756
display id: range1940756
version: 1
top level: tetR
direction: direction/forward
start: 1
end: 627
orientation: inline

https://synbiohub.org/public/igem/BBa_K510017_sequence/1
type: Sequence
persistent identity: BBa K510017 sequence
display id: BBa_K510017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510012_sequence/1
type: Sequence
persistent identity: BBa K510012 sequence
display id: BBa_K510012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732083_sequence/1
type: Sequence
persistent identity: BBa I732083 sequence
display id: BBa_I732083_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732080_sequence/1
type: Sequence
persistent identity: BBa I732080 sequence
display id: BBa_I732080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z