##gff-version 3
##sequence-region BBa_K510018 1 5758
BBa_K510018	.	plasmid	1	4549	.	+	0	ID=BBa_K510012;Name=BBa_K510012
BBa_K510018	.	CDS	1714	2574	.	+	0	ID=annotation2131795;Name=Ampicillin resistance (bla);Parent=BBa_K510012
BBa_K510018	.	CDS	3554	4087	.	+	0	ID=annotation2131805;Name=Gentamicin resistance;Parent=BBa_K510012
BBa_K510018	.	primer_binding_site	4410	4429	.	+	0	ID=annotation2131841;Name=VF2 (G00100) primer binding site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	3490	3537	.	+	0	ID=annotation2131802;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510018	.	primer_binding_site	157	176	.	+	0	ID=annotation2131786;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	646	905	.	+	0	ID=annotation2131797;Name=oriT;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	348	360	.	+	0	ID=annotation2131798;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	3176	3374	.	+	0	ID=annotation2131800;Name=Tn7-R end;Parent=BBa_K510012
BBa_K510018	.	stem_loop	22	93	.	+	0	ID=annotation2131863;Name=E. coli his operon terminator;Parent=BBa_K510012
BBa_K510018	.	stem_loop	4487	4520	.	+	0	ID=annotation2131866;Name=Terminator;Parent=BBa_K510012
BBa_K510018	.	engineered_region	3385	3479	.	+	0	ID=annotation2131801;Name=double terminator (BBa_B0014);Parent=BBa_K510012
BBa_K510018	.	sequence_feature	929	1310	.	+	0	ID=annotation2131796;Name=R6K replication origin;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	3548	3553	.	+	0	ID=annotation2131804;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	3542	3547	.	+	0	ID=annotation2131806;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	3163	3175	.	+	0	ID=annotation2131803;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	182	347	.	+	0	ID=annotation2131766;Name=Tn7-L end;Parent=BBa_K510012
BBa_K510018	.	engineered_region	2	21	.	+	0	ID=annotation2131765;Name=Suffix;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	4357	4404	.	+	0	ID=annotation2131856;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510018	.	sequence_feature	4345	4350	.	+	0	ID=annotation2131807;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510018	.	sequence_feature	4351	4355	.	+	0	ID=annotation2131808;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510018	.	engineered_region	4528	4549	.	+	0	ID=annotation2131839;Name=Prefix;Parent=BBa_K510012
BBa_K510018	.	engineered_region	4558	5758	.	+	0	ID=BBa_I732094;Name=BBa_I732094
BBa_K510018	.	ribosome_entry_site	4845	4856	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K510018	.	conserved_region	4849	4852	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K510018	.	terminator	5718	5758	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732094
BBa_K510018	.	engineered_region	5718	5758	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K510018	.	stem_loop	5725	5744	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K510018	.	polyA_site	5745	5758	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K510018	.	stop_codon	5751	5751	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K510018	.	sequence_feature	4558	4576	.	+	0	ID=BBa_I732007;Name=BBa_I732007;Parent=BBa_I732094
BBa_K510018	.	sequence_feature	4569	4574	.	+	0	ID=annotation1936908;Name=BamHI;Parent=BBa_I732007
BBa_K510018	.	sequence_feature	4560	4565	.	+	0	ID=annotation1936907;Name=BglII;Parent=BBa_I732007
BBa_K510018	.	terminator	5630	5709	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732094
BBa_K510018	.	engineered_region	5630	5709	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K510018	.	stem_loop	5641	5684	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K510018	.	ribosome_entry_site	4585	4596	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K510018	.	conserved_region	4589	4592	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K510018	.	CDS	4603	4836	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732094
BBa_K510018	.	CDS	4603	4836	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K510018	.	stop_codon	4831	4836	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K510018	.	start_codon	4603	4605	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K510018	.	engineered_region	4863	5621	.	+	0	ID=BBa_E0044;Name=BBa_E0044;Parent=BBa_I732094
>BBa_K510018
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggtt
aggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcggg
atcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagc
gcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaa
acttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttact
acggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaa
agatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccac
ctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatca
aacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagc
catgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctac
ttgcattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttc
catgggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcttctagagtactagaggaagatcttccggatccggtactag
agaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccc
tggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgat
cgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaa
gctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtc
ccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacat
acggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcgg
ttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggt
tatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgata
cccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaa
ctataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaac
attgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccag
acaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtt
tgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctgct
gctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata