LOCUS BBa_K510019 4678 bp DNA linear 16-Dec-2024 DEFINITION Improved Flip Flop (Module I) ACCESSION BBa_K510019 VERSION BBa_K510019.1 FEATURES Location/Qualifiers misc_feature 10..138 /label=Double terminator misc_feature 139..144 /label=Restriction Site: AflII misc_feature 147..154 /label=Restriction Site: AscI CDS 155..919 /label=GFP::Das4 misc_feature 161..205 /label=DAS4 RBS 926..938 /label=RBS misc_feature 940..945 /label=Restriction Site: BamHI CDS 947..2077 /label=LacI::Das4 misc_feature 953..997 /label=DAS4 RBS 2084..2096 /label=RBS promoter 2105..2153 /label=PcI misc_feature 2171..2176 /label=Restriction Site: Sma I promoter 2187..2386 /label=PLac RBS 2395..2407 /label=RBS CDS 2414..3127 /label=cI (Ts) misc_feature 3130..3135 /label=Restriction Site: NheI CDS 3139..3732 /label=RBS+OmpN::SspB misc_feature 3139..3234 /label=RBS+OmpN misc_feature 3735..3740 /label=Restriction Site: SacII misc_feature 3742..3837 /label=RBS+OmpN CDS 3742..4518 /label=RBS+OmpN::RFP misc_feature 4526..4531 /label=Restriction Site: StuI misc_feature 4534..4662 /label=Double terminator ORIGIN 1 gagatcattt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 61 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 121 gttttatttg atgcctggct taagtaggcg cgccctatta agaagcatca gcataatttt 181 cagagtagtt ctcgtcgttt gctgctttgt atagttcatc catgccatgt gtaatcccag 241 cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa 301 gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg 361 gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa 421 ttttgaagtt aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg 481 agttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac 541 cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct 601 tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct tcaggcatgg 661 cactcttgaa aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac 721 cataaccgaa agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa 781 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa 841 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa 901 gttcttctcc tttactcatc tagtactttc ctgtgtgacg gatccactat caagaagcat 961 cagcataatt ttcagagtag ttctcgtcgt ttgctgcctg cccgctttcc agtcgggaaa 1021 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 1081 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 1141 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 1201 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 1261 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 1321 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 1381 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 1441 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 1501 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 1561 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 1621 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 1681 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 1741 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 1801 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 1861 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 1921 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccacttct tcccgcgttt 1981 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 2041 catactctgc gacatcgtat aacgttactg gtttcaccta gtactttcct gtgtgactct 2101 agtagcaacc attatcaccg ccagaggtaa aatagtcaac acgcacggtg ttaagagcgc 2161 tggattacta cccgggacct atactacaat acgcaaaccg cctctccccg cgcgttggcc 2221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2281 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2341 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagtcacac 2401 aggaaagtac tagatgagca caaaaaagaa accattaaca caagagcagc ttgaggacgc 2461 acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc 2521 tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa 2581 tgcattaaat gcttataacg ccgcattgct tacaaaaatt ctcaaagtta gcgttgaaga 2641 atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc 2701 gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc 2761 acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa 2821 agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg 2881 ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga 2941 gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat 3001 cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg 3061 caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt 3121 tggctgatag ctagcaggta ggatgccttt gattcaacga atctgtagaa gttcaatctt 3181 ttgcaaataa gttaagtttt taaggataaa aaaatgaaaa gaaaagtatt ggcaatggat 3241 ttgtcacagc taacaccacg tcgtccctat ctgctgcgtg cattctatga gtggttgctg 3301 gataaccagc tcacgccgca cctggtggtg gatgtgacgc tcccaggcgt gcaggttcct 3361 atggaatatg cgcgtgacgg gcaaatcgta ctcaacattg cgccgcgtgc tgtcggcaat 3421 ctggaactgg cgaatgatga ggtgcgcttt aacgcgcgct ttggtggcat tccgcgtcag 3481 gtttctgtgc cgctggctgc cgtgctggct atctacgccc gtgaaaatgg tgcaggcacg 3541 atgtttgagc ctgaagctgc ctacgatgaa gatgccagca tcatgaatga tgaagaggca 3601 tcggcagaca acgaaaccgt tatgtcggtt atcgatggcg acaagccaga tcacgatgat 3661 gaaactcatc ctgacgatga acctccgcag ccaccacgcg gtggtcgacc ggcattacgc 3721 gttgtgaagt aataccgcgg gtaggatgcc tttgattcaa cgaatctgta gaagttcaat 3781 cttttgcaaa taagttaagt ttttaaggat aaaaaaatga aaagaaaagt attggcaatg 3841 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc 3901 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc 3961 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg 4021 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac 4081 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac 4141 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa 4201 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg 4261 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc 4321 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac 4381 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc 4441 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac 4501 tccaccggtg cttaataatg atactaggcc tagccaggca tcaaataaaa cgaaaggctc 4561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 4621 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tactagag //