BBa_K510019 1 BBa_K510019 Improved Flip Flop (Module I) 2011-09-18T11:00:00Z 2015-05-08T01:12:30Z It's a part which comes from different genes forming the bassic flip-flop with anothers improvements. Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure. false false _673_ 0 6159 9 It's complicated true false Victor Alvarez, Felix Reyes and Yolanda Gonzalez annotation2136071 1 RBS range2136071 1 2395 2407 annotation2136079 1 Restriction Site: StuI range2136079 1 4526 4531 annotation2136061 1 GFP::Das4 range2136061 1 155 919 annotation2136072 1 cI (Ts) range2136072 1 2414 3127 annotation2136060 1 Restriction Site: AscI range2136060 1 147 154 annotation2136076 1 Restriction Site: SacII range2136076 1 3735 3740 annotation2136065 1 LacI::Das4 range2136065 1 947 2077 annotation2136064 1 Restriction Site: BamHI range2136064 1 940 945 annotation2136078 1 RBS+OmpN::RFP range2136078 1 3742 4518 annotation2136062 1 DAS4 range2136062 1 161 205 annotation2136059 1 Restriction Site: AflII range2136059 1 139 144 annotation2136058 1 Double terminator range2136058 1 10 138 annotation2136077 1 RBS+OmpN range2136077 1 3742 3837 annotation2136063 1 RBS range2136063 1 926 938 annotation2136068 1 PcI range2136068 1 2105 2153 annotation2136069 1 Restriction Site: Sma I range2136069 1 2171 2176 annotation2136070 1 PLac range2136070 1 2187 2386 annotation2136080 1 Double terminator range2136080 1 4534 4662 annotation2136067 1 RBS range2136067 1 2084 2096 annotation2136073 1 Restriction Site: NheI range2136073 1 3130 3135 annotation2136075 1 RBS+OmpN range2136075 1 3139 3234 annotation2136074 1 RBS+OmpN::SspB range2136074 1 3139 3732 annotation2136066 1 DAS4 range2136066 1 953 997 BBa_K510019_sequence 1 gagatcatttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctattaagaagcatcagcataattttcagagtagttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcaggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttactcatctagtactttcctgtgtgacggatccactatcaagaagcatcagcataattttcagagtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacctagtactttcctgtgtgactctagtagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttaagagcgctggattactacccgggacctatactacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatagctagcaggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaataagttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctcccaggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggtgcaggcacgatgtttgagcctgaagctgcctacgatgaagatgccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttatcgatggcgacaagccagatcacgatgatgaaactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataccgcgggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaataagttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatgatactaggcctagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z