##gff-version 3
##sequence-region BBa_K510019 10 4662
BBa_K510019	.	sequence_feature	147	154	.	+	0	ID=annotation2136060;Name=Restriction Site: AscI
BBa_K510019	.	sequence_feature	4526	4531	.	+	0	ID=annotation2136079;Name=Restriction Site: StuI
BBa_K510019	.	ribosome_entry_site	2395	2407	.	+	0	ID=annotation2136071;Name=RBS
BBa_K510019	.	stop_codon	4534	4662	.	+	0	ID=annotation2136080;Name=Double terminator
BBa_K510019	.	sequence_feature	3139	3234	.	+	0	ID=annotation2136075;Name=RBS+OmpN
BBa_K510019	.	sequence_feature	3742	3837	.	+	0	ID=annotation2136077;Name=RBS+OmpN
BBa_K510019	.	tag	953	997	.	+	0	ID=annotation2136066;Name=DAS4
BBa_K510019	.	CDS	3742	4518	.	+	0	ID=annotation2136078;Name=RBS+OmpN::RFP
BBa_K510019	.	ribosome_entry_site	2084	2096	.	+	0	ID=annotation2136067;Name=RBS
BBa_K510019	.	sequence_feature	139	144	.	+	0	ID=annotation2136059;Name=Restriction Site: AflII
BBa_K510019	.	CDS	155	919	.	+	0	ID=annotation2136061;Name=GFP::Das4
BBa_K510019	.	CDS	947	2077	.	+	0	ID=annotation2136065;Name=LacI::Das4
BBa_K510019	.	CDS	2414	3127	.	+	0	ID=annotation2136072;Name=cI (Ts)
BBa_K510019	.	tag	161	205	.	+	0	ID=annotation2136062;Name=DAS4
BBa_K510019	.	sequence_feature	3130	3135	.	+	0	ID=annotation2136073;Name=Restriction Site: NheI
BBa_K510019	.	promoter	2105	2153	.	+	0	ID=annotation2136068;Name=PcI
BBa_K510019	.	sequence_feature	3735	3740	.	+	0	ID=annotation2136076;Name=Restriction Site: SacII
BBa_K510019	.	CDS	3139	3732	.	+	0	ID=annotation2136074;Name=RBS+OmpN::SspB
BBa_K510019	.	sequence_feature	2171	2176	.	+	0	ID=annotation2136069;Name=Restriction Site: Sma I
BBa_K510019	.	promoter	2187	2386	.	+	0	ID=annotation2136070;Name=PLac
BBa_K510019	.	stop_codon	10	138	.	+	0	ID=annotation2136058;Name=Double terminator
BBa_K510019	.	sequence_feature	940	945	.	+	0	ID=annotation2136064;Name=Restriction Site: BamHI
BBa_K510019	.	ribosome_entry_site	926	938	.	+	0	ID=annotation2136063;Name=RBS
>BBa_K510019
gagatcatttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaaca
acagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctat
taagaagcatcagcataattttcagagtagttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcc
cagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtgga
caggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacg
cttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacat
tgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgat
tctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagtt
ctttcctgtacataaccttcaggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagc
attgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaag
ggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaat
tcaacaagaattgggacaactccagtgaaaagttcttctcctttactcatctagtactttcctgtgtgacggatccact
atcaagaagcatcagcataattttcagagtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcg
tgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttc
accagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggttt
gccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcc
cactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttg
gcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagt
cgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccga
gacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgta
ccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgc
aggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaag
attgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcg
gcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacg
actgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccg
cgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgaca
tcgtataacgttactggtttcacctagtactttcctgtgtgactctagtagcaaccattatcaccgccagaggtaaaat
agtcaacacgcacggtgttaagagcgctggattactacccgggacctatactacaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcag
cttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcag
acaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcatt
gcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcg
gttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctg
agcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggct
tgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgac
cctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatca
gggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgt
ggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatagctagcaggtaggatgcctttgattcaacga
atctgtagaagttcaatcttttgcaaataagttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatgga
tttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccg
cacctggtggtggatgtgacgctcccaggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaaca
ttgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcg
tcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggtgcaggcacgatgtttgagcctgaa
gctgcctacgatgaagatgccagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttatcg
atggcgacaagccagatcacgatgatgaaactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggc
attacgcgttgtgaagtaataccgcgggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaata
agttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatggcttcctccgaagacgttatcaaagagttca
tgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgta
cgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcag
ttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtt
tcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtga
gttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgg
gaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacg
gtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccga
catcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcc
accggtgcttaataatgatactaggcctagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtactagag