##gff-version 3
##sequence-region BBa_K510020 1 5480
BBa_K510020	.	plasmid	1	4549	.	+	0	ID=BBa_K510012;Name=BBa_K510012
BBa_K510020	.	sequence_feature	4351	4355	.	+	0	ID=annotation2131808;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	4345	4350	.	+	0	ID=annotation2131807;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	3542	3547	.	+	0	ID=annotation2131806;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510020	.	primer_binding_site	157	176	.	+	0	ID=annotation2131786;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510012
BBa_K510020	.	engineered_region	2	21	.	+	0	ID=annotation2131765;Name=Suffix;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	929	1310	.	+	0	ID=annotation2131796;Name=R6K replication origin;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	3548	3553	.	+	0	ID=annotation2131804;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	3490	3537	.	+	0	ID=annotation2131802;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510020	.	primer_binding_site	4410	4429	.	+	0	ID=annotation2131841;Name=VF2 (G00100) primer binding site;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	3163	3175	.	+	0	ID=annotation2131803;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510020	.	stem_loop	22	93	.	+	0	ID=annotation2131863;Name=E. coli his operon terminator;Parent=BBa_K510012
BBa_K510020	.	engineered_region	3385	3479	.	+	0	ID=annotation2131801;Name=double terminator (BBa_B0014);Parent=BBa_K510012
BBa_K510020	.	CDS	3554	4087	.	+	0	ID=annotation2131805;Name=Gentamicin resistance;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	4357	4404	.	+	0	ID=annotation2131856;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510020	.	stem_loop	4487	4520	.	+	0	ID=annotation2131866;Name=Terminator;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	646	905	.	+	0	ID=annotation2131797;Name=oriT;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	348	360	.	+	0	ID=annotation2131798;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510020	.	engineered_region	4528	4549	.	+	0	ID=annotation2131839;Name=Prefix;Parent=BBa_K510012
BBa_K510020	.	CDS	1714	2574	.	+	0	ID=annotation2131795;Name=Ampicillin resistance (bla);Parent=BBa_K510012
BBa_K510020	.	sequence_feature	182	347	.	+	0	ID=annotation2131766;Name=Tn7-L end;Parent=BBa_K510012
BBa_K510020	.	sequence_feature	3176	3374	.	+	0	ID=annotation2131800;Name=Tn7-R end;Parent=BBa_K510012
BBa_K510020	.	engineered_region	4558	5480	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K510020	.	CDS	4638	5318	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K510020	.	CDS	4638	5312	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K510020	.	engineered_tag	5319	5343	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K510020	.	terminator	5440	5480	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K510020	.	engineered_region	5440	5480	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K510020	.	stop_codon	5473	5473	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K510020	.	stem_loop	5447	5466	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K510020	.	polyA_site	5467	5480	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K510020	.	terminator	5352	5431	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K510020	.	stem_loop	5363	5406	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K510020	.	engineered_region	5352	5431	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K510020	.	promoter	4558	4611	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K510020	.	promoter	4577	4582	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K510020	.	non_covalent_binding_site	4558	4576	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K510020	.	engineered_region	4558	4611	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K510020	.	promoter	4600	4605	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K510020	.	non_covalent_binding_site	4583	4601	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K510020	.	ribosome_entry_site	4620	4631	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K510020	.	conserved_region	4624	4627	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K510020
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggtt
aggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcggg
atcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagc
gcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaa
acttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttact
acggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaa
agatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccac
ctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatca
aacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagc
catgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctac
ttgcattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttc
catgggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata