LOCUS BBa_K510021 12194 bp DNA linear 22-Dec-2024 DEFINITION pUC18R6KT-miniTn7BB-Gm-Lux ACCESSION BBa_K510021 VERSION BBa_K510021.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix misc_feature 182..347 /label=Tn7-L end primer_bind 157..176 /label=VR (BBa_G00101) primer binding site CDS 1714..2574 /label=Ampicillin resistance (bla) misc_feature 929..1310 /label=R6K replication origin misc_feature 646..905 /label=oriT misc_feature 348..360 /label=SfiI restriction site misc_feature 3176..3374 /label=Tn7-R end misc_feature 3385..3479 /label=double terminator (BBa_B0014) misc_feature 3490..3537 /label=Flipase recombination sequence (FRT) misc_feature 3163..3175 /label=SfiI restriction site misc_feature 3548..3553 /label=NcoI restriction site CDS 3554..4087 /label=Gentamicin resistance misc_feature 3542..3547 /label=SphI restriction site misc_feature 4345..4350 /label=NcoI restriction site misc_feature 4351..4355 /label=SphI restriction site misc_feature 4528..4549 /label=Prefix primer_bind 4410..4429 /label=VF2 (G00100) primer binding site misc_feature 4357..4404 /label=Flipase recombination sequence (FRT) stem_loop 22..93 /label=E. coli his operon terminator stem_loop 4487..4520 /label=Terminator misc_feature 1..4549 /label=BBa_K510012 misc_feature 4558..5636 /label=AraC promoter 5600..5671 /label=PC misc_feature 5637..5767 /label=pBAD CDS 5788..7217 /label=luxC CDS 8186..9250 /label=luxA CDS 9289..10269 /label=luxB CDS 10383..11480 /label=luxE CDS 11484..12194 /label=luxG RBS 5768..5787 /label=putative rbs region RBS 7221..7225 /label=putative rbs RBS 9276..9281 /label=putative rbs CDS 7232..8135 /label=luxD RBS 8173..8178 /label=putative rbs RBS 10332..10338 /label=putative rbs RBS 11472..11476 /label=putative rbs misc_feature 4558..12194 /label=BBa_K325909 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgttaag gtatactttc 661 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt 721 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg 781 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac 841 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat 901 aagctctgat aacgcaggaa agaacatggg cagttcaacc tgttgatagt acgtactaag 961 ctctcatgtt tcacgtacta agctctcaag tttaacgtac taagctctca tgtttaacga 1021 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt 1081 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag 1141 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta 1201 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc 1261 tctcaaagca attttgagtg acacaggaac acttaacggc tgacaaccgg ctggtaacag 1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 1741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 1801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 1861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 1921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 1981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 2581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 2641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 2701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 2761 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2821 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2881 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2941 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 3001 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 3061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 3121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccgcct aggcctgtgg 3181 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 3241 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 3301 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 3361 aaagaggggc gtggggttcg cttgtcacac tggctcacct tcgggtgggc ctttctgcgt 3421 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttg 3481 ctagatcttg aagtacctat tccgaagttc ctattctcta aaaagtatag gaacttcaga 3541 ggcatgccca tggttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc 3601 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt 3661 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg 3721 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac 3781 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt 3841 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt 3901 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag 3961 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac 4021 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc 4081 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc 4141 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc 4201 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata 4261 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaattcgat ccattgctgt 4321 tgacaaaggg aatcagggga tcttccatgg gcatgcgaag tacctattcc gaagttccta 4381 ttctctaaaa agtataggaa cttcagagct gccacctgac gtctaagaaa ccattattat 4441 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc 4501 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt actagagtta 4561 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg 4621 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac 4681 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct 4741 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg 4801 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct 4861 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg 4921 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat 4981 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag 5041 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat 5101 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg 5161 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag 5221 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc 5281 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc 5341 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta 5401 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag 5461 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct 5521 cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac 5581 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat 5641 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 5701 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg 5761 ggctagcagg gaaaataatg aataaatgta ttccaatgat aattaatgga atgattcaag 5821 attttgataa ttatgcatat aaagaagtta aactaaataa tgataataga gtaaaattat 5881 ctgtcattac tgaaagttca gtttcaaaaa cattaaatat caaagataga attaatctaa 5941 atttaaatca gattgtgaat tttttatata ccgttggtca acgatggaaa agtgaagaat 6001 ataatcggcg acgaacctat atccgtgagt taaaaacata tcttggttat tctgatgaaa 6061 tggcaagatt agaagcgaat tggattgcaa tgttattgtg ctctaaaagt gctttgtatg 6121 acattgttaa ttatgatttg ggctctatac acgtattaga tgaatggctt ccacgtggtg 6181 attgctatgt taaagcacaa ccgaaaggtg tttctgttca cttgttagct ggtaatgttc 6241 cattatcagg agtgacatct attttgcgtg ctattttaac aaaaaatgag tgcattatta 6301 aaacttcgtc ttcagatcct tttactgcaa acgctttagt ttccagtttt attgatgtta 6361 atgcagacca tccaataacc aaatcaatgt ctgttatgta ttggccgcat gatgaagata 6421 tgactctatc tcaaagaata atgaatcatg ccgacgtggt tattgcttgg ggtggagacg 6481 aggcgattaa atgggcggta aaatattcac caccgcatgt cgatattctg aaatttggac 6541 caaagaaaag cttaagtatt attgaagctc ctaaagatat agaagccgca gcaatggggg 6601 ttgctcatga tatttgtttc tatgaccagc aagcctgctt ctctactcaa gacgtttatt 6661 atataggaga taatttacct ttatttttaa atgaacttga aaaacagcta gatcgatacg 6721 cgaaaatttt accaaaaggt tcaaatagtt ttgatgaaaa agcggcgttt actcttactg 6781 aaaaagaaag tctatttgct ggatatgaag tgagaaaggg agataagcaa gcttggttaa 6841 tagtcgtatc acctacaaat agctttggaa atcaaccgct atcacgaagt gtgtatgttc 6901 atcaagtatc tgatattaaa gagataattc cttttgttaa taaaaataga acacaaactg 6961 tttctattta tccttgggaa gcgtcattaa aatatcgaga taaattagca agaagtggag 7021 ttgaaagaat tgttgaatca ggcatgaata atattttcag agttggaggg gctcatgatt 7081 cattatctcc tctccagtac ctagttaggt ttgtatcgca tgagagacca tttaattata 7141 cgacaaaaga tgttgcggtt gaaatcgaac aaacacgtta cttagaggaa gataagtttt 7201 tagtttttgt cccatagtta aaggaaatta tatgaaagat gaaagtgctt tttttacgat 7261 tgatcacatc atcaagcttg ataatggtca gtctatccga gtttgggaaa cactccctaa 7321 aaagaacgta ccagagaaaa aacatacaat acttattgct tcgggttttg ctagaagaat 7381 ggatcatttt gcaggtcttg ctgagtattt atctactaac ggttttcatg tcattcgcta 7441 cgattctttg catcatgttg gattaagcag tggatgtata aatgaattta cgatgtcgat 7501 tggaaaaaat agcctgctta cagtcgtaga ttggcttaca gatcatggtg tcgaacgaat 7561 agggctgatt gctgctagtt tgtcagcgag aatcgcctat gaggtagtaa ataaaattaa 7621 attatcattt ttaattacgg ccgtaggtgt cgttaatctt agagatacat tagaaaaagc 7681 attggagtat gactatttgc aattacctat ttcagagtta ccagaagatc ttgactttga 7741 aggtcataat ttaggatcgg aggtctttgt tacagattgc tttaaacata attgggacac 7801 gttagactcg acacttaata gtgttaaagg attagcgatt ccatttattg cttttactgc 7861 aaacgatgat agttgggtaa agcaaagtga agttatagag ctcattgata gcattgaatc 7921 tagtaattgt aagctctatt cgctaattgg aagttcacat gatcttgggg aaaatttggt 7981 tgtattaaga aatttttatc aatcagtgac gaaggcagcc ttagcattag atgatggttt 8041 attggattta gagatagaca ttattgaacc tcgatttgag gacgttacaa gtattactgt 8101 taaggagcgt agattaaaaa atgaaattga aaatgaatta ttagaattgg cttaaataaa 8161 cagaatcacc aaaaaggaat agagtatgaa gtttggaaat atttgttttt cgtatcaacc 8221 accaggtgaa actcataagc aagtaatgga tcgctttgtt cggcttggta tcgcctcaga 8281 agaggtaggg tttgatacat attggacctt agaacatcat tttacagagt ttggtcttac 8341 gggaaattta tttgttgctg cggctaacct gttaggaaga actaaaacat taaatgttgg 8401 cactatgggg gttgttattc cgacagcaca cccagttcga cagttagaag acgttttatt 8461 attagatcaa atgtcgaaag gtcgttttaa ttttggaacc gttcgagggc tataccataa 8521 agattttcga gtatttggtg ttgatatgga agagtctcga gcaattactc aaaatttcta 8581 ccagatgata atggaaagct tacagacagg aaccattagc tctgatagtg attacattca 8641 atttcctaag gttgatgtat atcccaaagt gtactcaaaa aatgtaccaa cctgtatgac 8701 tgctgagtcc gcaagtacga cagaatggct agcaatacaa gggctaccaa tggttcttag 8761 ttggattatt ggtactaatg aaaaaaaagc acagatggaa ctctataatg aaattgcgac 8821 agaatatggt catgatatat ctaaaataga tcattgtatg acttatattt gttctgttga 8881 tgatgatgca caaaaggcgc aagatgtttg tcgggagttt ctgaaaaatt ggtatgactc 8941 atatgtaaat gcgaccaata tctttaatga tagcaatcaa actcgtggtt atgattatca 9001 taaaggtcaa tggcgtgatt ttgttttaca aggacataca aacaccaatc gacgtgttga 9061 ttatagcaat ggtattaacc ctgtaggcac tcctgagcag tgtattgaaa tcattcaacg 9121 tgatattgat gcaacgggta ttacaaacat tacatgcgga tttgaagcta atggaactga 9181 agatgaaata attgcttcca tgcgacgctt tatgacacaa gtcgctcctt tcttaaaaga 9241 acctaaataa attacttatt tgatactaga gataataagg aacaagttat gaaatttgga 9301 ttattttttc taaactttca gaaagatgga ataacatctg aagaaacgtt ggataatatg 9361 gtaaagactg tcacgttaat tgattcaact aaatatcatt ttaatactgc ctttgttaat 9421 gaacatcact tttcaaaaaa tggtattgtt ggagcaccta ttaccgcagc tggtttttta 9481 ttagggttaa caaataaatt acatattggt tcattaaatc aagtaattac cacccatcac 9541 cctgtacgtg tagcagaaga agccagttta ttagatcaaa tgtcagaggg acgcttcatt 9601 cttggtttta gtgactgcga aagtgatttc gaaatggaat tttttagacg tcatatctca 9661 tcaaggcaac aacaatttga agcatgctat gaaataatta atgacgcatt aactacaggt 9721 tattgtcatc cccaaaacga cttttatgat tttccaaagg tttcaattaa tccacactgt 9781 tacagtgaga atggacctaa gcaatatgta tccgctacat caaaagaagt cgtcatgtgg 9841 gcagcgaaaa aggcactgcc tttaacattt aagtgggagg ataatttaga aaccaaagaa 9901 cgctatgcaa ttctatataa taaaacagca caacaatatg gtattgatat ttcggatgtt 9961 gatcatcaat taactgtaat tgcgaactta aatgctgata gaagtacggc tcaagaagaa 10021 gtgagagaat acttaaaaga ctatatcact gaaacttacc ctcaaatgga cagagatgaa 10081 aaaattaact gcattattga agagaatgca gttgggtctc atgatgacta ttatgaatcg 10141 acaaaattag cagtggaaaa aacagggtct aaaaatattt tattatcctt tgaatcaatg 10201 tccgatatta aagatgtaaa agatattatt gatatgttga accaaaaaat cgaaatgaat 10261 ttaccataat aaaattaaag gcaatttcta tattagattg cctttttaaa tttctgttga 10321 tattaggtat tactggagag ggtatgactg tccatactga atataaaaga aatcaaatca 10381 ttgctagttc agaaattgat gatcttatct ttatgacgaa accacaagag tggtcatttg 10441 aagagcaaaa agaaatacgg gataaattag ttcgtgaggc tttttatttt cactacaata 10501 gaaatgaaga atatagaaat tattgtatca atcagcatgt gagtgataat ttacacacta 10561 ttgatgaaat acccgtgttt ccaacatctg tttttaaata taagaaatta catactgtca 10621 cagccgagga cattgaaaat tggtatacaa gtagtggaac tcgtggagta aaaagtcata 10681 ttgcacgtga tcgtcttagc attgaacgct tgcttggttc tgtcaacttc ggaatgaaat 10741 acgttggaga ttggtttgag catcaaatgg aattgataaa tttaggacca gatagattca 10801 atacaaataa tatttggttt aaatatgtca tgagtttggt cgagttactt tatccgactg 10861 aatttacagt tgataatgac aaaatagatt ttgaaaaaac agtaaaacat ctatttagaa 10921 ttaagaatag taaaaaagac atttgcttaa ttgggccacc attttttgtg tatcttttgt 10981 gccaatatat gaaagaaaac aatattgaat ttaaaggagg agatagagta catattatta 11041 ctggtggagg atggaaatct aatcagaatg actctttaga tcgtgctgat tttaatcaat 11101 tattaatgga tactttccaa ctcgacaaga ttaatcaaat tagagatacc tttaatcaag 11161 ttgagcttaa tacttgtttt tttgaagatg aatttcaaag aaaacatgtt ccaccgtggg 11221 tatatgctcg ggctcttgat cctgaaacct tgaaacccgt agcagatggt gagatcgggt 11281 tgttaagtta tatggacgcc tcatcaactg cttaccctgc ttttattgtt actgatgata 11341 tcggtattgt aaaagaaatt agagaaccag atccttaccc aggggtaact gttgagattg 11401 ttcggcgctt aaatacacgt gcgcaaaaag gatgcgcgct ctctatggct aatgtcatac 11461 aaaagaatat caaggattaa gttatgattg ttgatggtag agtttcaaaa atagtattag 11521 cgtcaataag aaataatata tataaggtat ttattactgt aaattcacca ataaagttca 11581 tcgctggaca atttgtaatg gtcacgatta atgggaaaaa atgccctttt tcaattgcga 11641 attgcccgac aaaaaattac gaaatagaat tgcatattgg tagttcgaat agagactgct 11701 cattggatat tatcgaatat tttgtcgatg ctcttgttga ggaagtcgca attgagttag 11761 atgctcccca tggaaacgct tggttacggt ctgaaagtaa taacccattg ctattaattg 11821 cgggaggtac aggtttatca tatataaata gcattctaac aaattgctta aataggaata 11881 tacctcaaga tatttatctt tactggggag taaaagacag ttctcttttg tatgaagatg 11941 aagagttact aaacttatca ctaaacaaca aaaactttca ttatattcct gttattgaag 12001 ataaaagtga agaatggata gggagaaaag gcactgttct tgatgctgtc atggaagatt 12061 ttactgatct tacttatttt gatatttatg tttgtggacc cttcatgatg gctaaaacag 12121 caaaagaaaa attaattgaa gagaaaaaag caaagtcaga acagatgttt gccgatgctt 12181 ttgcatacgt ataa //