BBa_K510021
type: ComponentDefinition
persistent identity: BBa K510021
display id: BBa_K510021
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm-Lux
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The BBa_K325909 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K325909
component: BBa_K510012
sequence annotation: BBa_K510012
sequence annotation: BBa_K325909
sequence: https://synbiohub.org/public/igem/BBa_K510021_sequence/1

BBa_K325909
type: Component
persistent identity: component2134780
display id: component2134780
version: 1
top level: BBa_K510021
definition: BBa_K325909
access: public

BBa_K510012
type: Component
persistent identity: component2134764
display id: component2134764
version: 1
top level: BBa_K510021
definition: BBa_K510012
access: public

BBa_K510012
type: SequenceAnnotation
persistent identity: annotation2134764
display id: annotation2134764
version: 1
top level: BBa_K510021
location: https://synbiohub.org/public/igem/BBa_K510021/annotation2134764/range2134764/1
component: BBa_K510012

https://synbiohub.org/public/igem/BBa_K510021/annotation2134764/range2134764/1
type: Range
persistent identity: range2134764
display id: range2134764
version: 1
top level: BBa_K510021
direction: direction/reverse
start: 1
end: 4549
orientation: inline

BBa_K325909
type: SequenceAnnotation
persistent identity: annotation2134780
display id: annotation2134780
version: 1
top level: BBa_K510021
location: https://synbiohub.org/public/igem/BBa_K510021/annotation2134780/range2134780/1
component: BBa_K325909

https://synbiohub.org/public/igem/BBa_K510021/annotation2134780/range2134780/1
type: Range
persistent identity: range2134780
display id: range2134780
version: 1
top level: BBa_K510021
direction: direction/reverse
start: 4558
end: 12194
orientation: inline

BBa_K325909
type: ComponentDefinition
persistent identity: BBa K325909
display id: BBa_K325909
version: 1
was generated by: iGEM to SBOL conversion
description: Lux Operon (under pBAD) Vibrio Fischeri
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:03Z
mutable provenance: a
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: G28
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: a
star: false
creator: Theo Sanderson and Will Handley
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: AraC
sequence annotation: PC
sequence annotation: pBAD
sequence annotation: putative rbs region
sequence annotation: luxC
sequence annotation: putative rbs
sequence annotation: luxD
sequence annotation: luxA
sequence annotation: luxB
sequence annotation: luxE
sequence annotation: luxG
sequence: https://synbiohub.org/public/igem/BBa_K325909_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2111897
display id: annotation2111897
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2111897/range2111897/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K325909/annotation2111897/range2111897/1
type: Range
persistent identity: range2111897
display id: range2111897
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2112036
display id: annotation2112036
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112036/range2112036/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K325909/annotation2112036/range2112036/1
type: Range
persistent identity: range2112036
display id: range2112036
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1043
end: 1114
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2112038
display id: annotation2112038
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112038/range2112038/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K325909/annotation2112038/range2112038/1
type: Range
persistent identity: range2112038
display id: range2112038
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1080
end: 1210
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2112060
display id: annotation2112060
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1
type: Range
persistent identity: range2112060
display id: range2112060
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1211
end: 1230
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2112039
display id: annotation2112039
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1
type: Range
persistent identity: range2112039
display id: range2112039
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1231
end: 2660
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112061
display id: annotation2112061
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112061/range2112061/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112061/range2112061/1
type: Range
persistent identity: range2112061
display id: range2112061
version: 1
top level: BBa_K325909
direction: direction/forward
start: 2664
end: 2668
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2112076
display id: annotation2112076
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1
type: Range
persistent identity: range2112076
display id: range2112076
version: 1
top level: BBa_K325909
direction: direction/forward
start: 2675
end: 3578
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112117
display id: annotation2112117
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112117/range2112117/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112117/range2112117/1
type: Range
persistent identity: range2112117
display id: range2112117
version: 1
top level: BBa_K325909
direction: direction/forward
start: 3616
end: 3621
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2112056
display id: annotation2112056
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1
type: Range
persistent identity: range2112056
display id: range2112056
version: 1
top level: BBa_K325909
direction: direction/forward
start: 3629
end: 4693
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112062
display id: annotation2112062
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1
type: Range
persistent identity: range2112062
display id: range2112062
version: 1
top level: BBa_K325909
direction: direction/forward
start: 4719
end: 4724
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2112057
display id: annotation2112057
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1
type: Range
persistent identity: range2112057
display id: range2112057
version: 1
top level: BBa_K325909
direction: direction/forward
start: 4732
end: 5712
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112119
display id: annotation2112119
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1
type: Range
persistent identity: range2112119
display id: range2112119
version: 1
top level: BBa_K325909
direction: direction/forward
start: 5775
end: 5781
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2112058
display id: annotation2112058
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1
type: Range
persistent identity: range2112058
display id: range2112058
version: 1
top level: BBa_K325909
direction: direction/forward
start: 5826
end: 6923
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112122
display id: annotation2112122
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1
type: Range
persistent identity: range2112122
display id: range2112122
version: 1
top level: BBa_K325909
direction: direction/forward
start: 6915
end: 6919
orientation: inline

luxG
type: SequenceAnnotation
persistent identity: annotation2112059
display id: annotation2112059
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1
type: Range
persistent identity: range2112059
display id: range2112059
version: 1
top level: BBa_K325909
direction: direction/forward
start: 6927
end: 7637
orientation: inline

BBa_K510012
type: ComponentDefinition
persistent identity: BBa K510012
display id: BBa_K510012
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: pUC18R6KT-miniTn7BB-Gm is a vehicle vector for the minitransposon miniTn7BB-Gm. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacteria. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. This plasmid is replicative only in E. coli strains expressing the pir gene thanks to its R6K replication origin. Also it has an origin for transference (oriT) for conjugation purposes. This plasmid can be used to maintain the miniTn7-Gm transposon in E. coli pir+ strains and may be used for transposition into the genomes of any bacteria without the pir gen, in which it is non-replicative. As a BioBrick cloning vector, pUC18R6KT-miniTn7BB-Gm is fully compatible with Assembly Standard 10. The complete transposon is flanked by SfiI restriction sites to facilitate subcloning in other vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: oriT
sequence annotation: R6K replication origin
sequence annotation: Ampicillin resistance (bla)
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restriction site
sequence annotation: Gentamicin resistance
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510012_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2131765
display id: annotation2131765
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
type: Range
persistent identity: range2131765
display id: range2131765
version: 1
top level: BBa_K510012
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2131863
display id: annotation2131863
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
type: Range
persistent identity: range2131863
display id: range2131863
version: 1
top level: BBa_K510012
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131786
display id: annotation2131786
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
type: Range
persistent identity: range2131786
display id: range2131786
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2131766
display id: annotation2131766
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
type: Range
persistent identity: range2131766
display id: range2131766
version: 1
top level: BBa_K510012
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131798
display id: annotation2131798
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
type: Range
persistent identity: range2131798
display id: range2131798
version: 1
top level: BBa_K510012
direction: direction/forward
start: 348
end: 360
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2131797
display id: annotation2131797
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
type: Range
persistent identity: range2131797
display id: range2131797
version: 1
top level: BBa_K510012
direction: direction/forward
start: 646
end: 905
orientation: inline

R6K replication origin
type: SequenceAnnotation
persistent identity: annotation2131796
display id: annotation2131796
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
type: Range
persistent identity: range2131796
display id: range2131796
version: 1
top level: BBa_K510012
direction: direction/forward
start: 929
end: 1310
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2131795
display id: annotation2131795
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
type: Range
persistent identity: range2131795
display id: range2131795
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 1714
end: 2574
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131803
display id: annotation2131803
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
type: Range
persistent identity: range2131803
display id: range2131803
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3163
end: 3175
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2131800
display id: annotation2131800
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
type: Range
persistent identity: range2131800
display id: range2131800
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3176
end: 3374
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2131801
display id: annotation2131801
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
type: Range
persistent identity: range2131801
display id: range2131801
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3385
end: 3479
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131802
display id: annotation2131802
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
type: Range
persistent identity: range2131802
display id: range2131802
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3490
end: 3537
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131806
display id: annotation2131806
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
type: Range
persistent identity: range2131806
display id: range2131806
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3542
end: 3547
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131804
display id: annotation2131804
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
type: Range
persistent identity: range2131804
display id: range2131804
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3548
end: 3553
orientation: inline

Gentamicin resistance
type: SequenceAnnotation
persistent identity: annotation2131805
display id: annotation2131805
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
type: Range
persistent identity: range2131805
display id: range2131805
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 3554
end: 4087
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131807
display id: annotation2131807
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
type: Range
persistent identity: range2131807
display id: range2131807
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4345
end: 4350
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131808
display id: annotation2131808
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
type: Range
persistent identity: range2131808
display id: range2131808
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4351
end: 4355
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131856
display id: annotation2131856
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
type: Range
persistent identity: range2131856
display id: range2131856
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4357
end: 4404
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131841
display id: annotation2131841
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
type: Range
persistent identity: range2131841
display id: range2131841
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4410
end: 4429
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2131866
display id: annotation2131866
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
type: Range
persistent identity: range2131866
display id: range2131866
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4487
end: 4520
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2131839
display id: annotation2131839
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
type: Range
persistent identity: range2131839
display id: range2131839
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4528
end: 4549
orientation: inline

https://synbiohub.org/public/igem/BBa_K510021_sequence/1
type: Sequence
persistent identity: BBa K510021 sequence
display id: BBa_K510021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K325909_sequence/1
type: Sequence
persistent identity: BBa K325909 sequence
display id: BBa_K325909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcagggaaaataatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510012_sequence/1
type: Sequence
persistent identity: BBa K510012 sequence
display id: BBa_K510012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z