BBa_K510031 1 BBa_K510031 GFP-Tadh1- tetO4- Tact1 2011-09-19T11:00:00Z 2015-05-08T01:12:30Z This composite part is formed by four main elements. Coding sequence of GFP reporter protein, followed by the transcriptional terminator of ADH1 gene. The next part is denominated tetO4, that consist of four 19 nucleotices tetR operator sequences, alternated with spacers. And the last part is the Act1 transcriptional terminator that function as insulator. This part should be interted behind a promoter. In association with Tadh1-tetO2 part, it functions as a transcription regulator, by compacting intermediate sequence. false false _673_ 0 6162 9 Not in stock false false Paola Gallardo, Rafael R. Daga annotation2136187 1 tetO range2136187 1 1078 1096 annotation2136184 1 tetO range2136184 1 952 970 annotation2136186 1 tetO range2136186 1 1036 1054 annotation2136188 1 act1 terminator range2136188 1 1120 1261 annotation2136183 1 adh1 terminator range2136183 1 735 914 annotation2136185 1 tetO range2136185 1 994 1012 annotation2136182 1 GFP range2136182 1 1 726 BBa_K510031_sequence 1 atgtctaagggtgaagaactttttactggtgttgttcctattcttgttgaacttgatggtgatgttaacggtcataagttttctgtttctggtgaaggtgaaggtgatgctacttatggtaaacttactcttaagtttatttgcactactggtaagttgcctgttccttggcctactttagttactacttttggttatggtgttcaatgctttgctcgttaccctgatcatatgaagcaacatgatttttttaagtctgctatgcctgaaggttatgttcaagaacgtactattttttttaaagatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactcttgttaatcgtattgaacttaagggtattgattttaaagaagatggaaatattcttggtcataagcttgaatacaactacaattctcataacgtttacattatggctgataagcaaaagaacggtattaaggttaactttaagattcgtcataatattgaagatggttctgttcaacttgctgatcattaccaacaaaacactcctattggtgatggtcctgttcttcttcctgataaccattacctttctactcaatctgctctttctaaggatcctaacgaaaagcgagatcatatggttcttttggaatttgttactgctgctggtattactcatggtatggatgaactttacaagactacttcttaaggcgcgccgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccggcagatcaattcctcgatcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccacgtttctaatctctgcttttgtgcgcgtatgtttatgtatgtacctctctctctatttctatttttaaaccaccctctcaataaaataaaaataataaagtatttttaaggaaaagacgtgtttaagcactgactttatctacgaattc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z