LOCUS BBa_K510039 5285 bp DNA linear 13-Jun-2024 DEFINITION pUC18Sfi-miniTn7BB-Gm-tetR ACCESSION BBa_K510039 VERSION BBa_K510039.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix stem_loop 22..93 /label=E. coli his operon terminator primer_bind 157..176 /label=VR (BBa_G00101) primer binding site misc_feature 182..347 /label=Tn7-L end misc_feature 348..360 /label=SfiI restriction site CDS 966..1826 /label=Ampicillin resistance (bla) misc_feature 1965..2644 /label=ColEI (pUC18) Replication origin misc_feature 3002..3014 /label=SfiI restriction site misc_feature 3014..3213 /label=Tn7-R end misc_feature 3329..3376 /label=Flipase recombination sequence (FRT) CDS 3393..3926 /label=Gentamycin resistance misc_feature 4196..4243 /label=Flipase recombination sequence (FRT) misc_feature 4367..4387 /label=Prefix misc_feature 3224..3318 /label=double terminator (BBa_B0014) misc_feature 3381..3386 /label=SphI restriction site misc_feature 3387..3392 /label=NcoI restrictino site misc_feature 4184..4189 /label=NcoI restriction site misc_feature 4190..4195 /label=SphI restriction site primer_bind 4249..4268 /label=VF2 (G00100) primer binding site stem_loop 4326..4359 /label=Terminator misc_feature 1..4388 /label=BBa_K510000 misc_feature 4397..4433 /label=P(Lac) IQ promoter 4399..4404 /label=-35 promoter 4422..4427 /label=-10 promoter 4397..4433 /label=BBa_I14032 misc_feature 4446..4449 /label=conserved RBS 4442..4453 /label=BBa_B0034 CDS 4460..5086 /label=tetR CDS 4460..5086 /label=BBa_I732080 stem_loop 5106..5149 /label=stem_loop misc_feature 5095..5174 /label=BBa_B0010 terminator 5095..5174 /label=BBa_B0010 stem_loop 5190..5209 /label=T7 TE misc_feature 5216..5216 /label=stop polyA_site 5210..5223 /label=polya misc_feature 5183..5223 /label=BBa_B0012 terminator 5183..5223 /label=BBa_B0012 misc_feature 5232..5250 /label=TetR 1 misc_feature 5232..5285 /label=BBa_R0040 promoter 5251..5256 /label=-35 misc_feature 5257..5275 /label=TetR 2 promoter 5274..5279 /label=-10 promoter 5232..5285 /label=BBa_R0040 misc_feature 4397..5285 /label=BBa_I732083 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg 2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 3001 tggccgccta ggcctgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 3061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 3121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 3181 gcaaattgcc cgtcgtatta aagaggggcg tggggttcgc ttgtcacact ggctcacctt 3241 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat 3301 ccggcttttt tattatttgc tagatcttga agtacctatt ccgaagttcc tattctctaa 3361 aaagtatagg aacttcagag gcatgcccat ggttaggtgg cggtacttgg gtcgatatca 3421 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca 3481 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg 3541 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca 3601 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg 3661 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag 3721 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca 3781 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat 3841 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc 3901 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 3961 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata 4021 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga 4081 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt 4141 caattcgatc cattgctgtt gacaaaggga atcaggggat cttccatggg catgcgaagt 4201 acctattccg aagttcctat tctctaaaaa gtataggaac ttcagagctg ccacctgacg 4261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 4321 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 4381 ttctagagta ctagagtggt gcaaaacctt tcgcggtatg gcatgatagc gcctactaga 4441 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 4501 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 4561 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 4621 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 4681 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 4741 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 4801 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 4861 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 4921 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 4981 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 5041 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc taataatact agagccaggc 5101 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 5161 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 5221 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 5281 agcac //