##gff-version 3
##sequence-region BBa_K510040 1 5597
BBa_K510040	.	engineered_region	4397	5597	.	+	0	ID=BBa_I732094;Name=BBa_I732094
BBa_K510040	.	CDS	4442	4675	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732094
BBa_K510040	.	stop_codon	4670	4675	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K510040	.	CDS	4442	4675	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K510040	.	start_codon	4442	4444	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K510040	.	terminator	5469	5548	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732094
BBa_K510040	.	engineered_region	5469	5548	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K510040	.	stem_loop	5480	5523	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K510040	.	sequence_feature	4397	4415	.	+	0	ID=BBa_I732007;Name=BBa_I732007;Parent=BBa_I732094
BBa_K510040	.	sequence_feature	4399	4404	.	+	0	ID=annotation1936907;Name=BglII;Parent=BBa_I732007
BBa_K510040	.	sequence_feature	4408	4413	.	+	0	ID=annotation1936908;Name=BamHI;Parent=BBa_I732007
BBa_K510040	.	ribosome_entry_site	4684	4695	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K510040	.	conserved_region	4688	4691	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K510040	.	terminator	5557	5597	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732094
BBa_K510040	.	stop_codon	5590	5590	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K510040	.	polyA_site	5584	5597	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K510040	.	stem_loop	5564	5583	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K510040	.	engineered_region	5557	5597	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K510040	.	ribosome_entry_site	4424	4435	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732094
BBa_K510040	.	conserved_region	4428	4431	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K510040	.	engineered_region	4702	5460	.	+	0	ID=BBa_E0044;Name=BBa_E0044;Parent=BBa_I732094
BBa_K510040	.	plasmid	1	4388	.	+	0	ID=BBa_K510000;Name=BBa_K510000
BBa_K510040	.	sequence_feature	1965	2644	.	+	0	ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000
BBa_K510040	.	engineered_region	4367	4387	.	+	0	ID=annotation2137423;Name=Prefix;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	348	360	.	+	0	ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	3381	3386	.	+	0	ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	3002	3014	.	+	0	ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	182	347	.	+	0	ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	4190	4195	.	+	0	ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	4196	4243	.	+	0	ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510040	.	sequence_feature	4184	4189	.	+	0	ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000
BBa_K510040	.	CDS	966	1826	.	+	0	ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000
BBa_K510040	.	sequence_feature	3329	3376	.	+	0	ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510040	.	sequence_feature	3387	3392	.	+	0	ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000
BBa_K510040	.	stem_loop	4326	4359	.	+	0	ID=annotation2137449;Name=Terminator;Parent=BBa_K510000
BBa_K510040	.	primer_binding_site	157	176	.	+	0	ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000
BBa_K510040	.	sequence_feature	3014	3213	.	+	0	ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000
BBa_K510040	.	engineered_region	2	21	.	+	0	ID=annotation2137411;Name=Suffix;Parent=BBa_K510000
BBa_K510040	.	engineered_region	3224	3318	.	+	0	ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000
BBa_K510040	.	CDS	3393	3926	.	+	0	ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000
BBa_K510040	.	primer_binding_site	4249	4268	.	+	0	ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000
BBa_K510040	.	stem_loop	22	93	.	+	0	ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000
>BBa_K510040
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt
gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac
gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc
cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg
tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct
atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc
cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat
attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt
ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg
gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc
tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt
ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca
tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac
gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt
agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta
ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac
caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct
gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga
gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt
tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat
taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa
gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg
gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac
tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca
aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg
tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc
gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg
gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact
tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg
gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga
tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta
actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac
atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat
gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg
cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat
gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagaggaagatcttccggatccggtactagaga
aagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctgg
cgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgc
ccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagct
ggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctgctgct
gtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata