##gff-version 3
##sequence-region BBa_K510041 1 5319
BBa_K510041	.	engineered_region	4397	5319	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K510041	.	CDS	4477	5157	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K510041	.	CDS	4477	5151	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K510041	.	engineered_tag	5158	5182	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K510041	.	ribosome_entry_site	4459	4470	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K510041	.	conserved_region	4463	4466	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K510041	.	terminator	5191	5270	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K510041	.	stem_loop	5202	5245	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K510041	.	engineered_region	5191	5270	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K510041	.	promoter	4397	4450	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K510041	.	engineered_region	4397	4450	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K510041	.	promoter	4416	4421	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K510041	.	non_covalent_binding_site	4397	4415	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K510041	.	promoter	4439	4444	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K510041	.	non_covalent_binding_site	4422	4440	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K510041	.	terminator	5279	5319	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K510041	.	stem_loop	5286	5305	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K510041	.	stop_codon	5312	5312	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K510041	.	polyA_site	5306	5319	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K510041	.	engineered_region	5279	5319	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K510041	.	plasmid	1	4388	.	+	0	ID=BBa_K510000;Name=BBa_K510000
BBa_K510041	.	sequence_feature	3387	3392	.	+	0	ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000
BBa_K510041	.	engineered_region	4367	4387	.	+	0	ID=annotation2137423;Name=Prefix;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	3014	3213	.	+	0	ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000
BBa_K510041	.	engineered_region	2	21	.	+	0	ID=annotation2137411;Name=Suffix;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	3329	3376	.	+	0	ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510041	.	sequence_feature	3381	3386	.	+	0	ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	4190	4195	.	+	0	ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	3002	3014	.	+	0	ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510041	.	stem_loop	22	93	.	+	0	ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	4196	4243	.	+	0	ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510041	.	engineered_region	3224	3318	.	+	0	ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000
BBa_K510041	.	sequence_feature	182	347	.	+	0	ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000
BBa_K510041	.	stem_loop	4326	4359	.	+	0	ID=annotation2137449;Name=Terminator;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	348	360	.	+	0	ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510041	.	CDS	966	1826	.	+	0	ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000
BBa_K510041	.	sequence_feature	1965	2644	.	+	0	ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000
BBa_K510041	.	primer_binding_site	4249	4268	.	+	0	ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000
BBa_K510041	.	CDS	3393	3926	.	+	0	ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000
BBa_K510041	.	primer_binding_site	157	176	.	+	0	ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000
BBa_K510041	.	sequence_feature	4184	4189	.	+	0	ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000
>BBa_K510041
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt
gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac
gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc
cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg
tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct
atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc
cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat
attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt
ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg
gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc
tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt
ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca
tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac
gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt
agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta
ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac
caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct
gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga
gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt
tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat
taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa
gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg
gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac
tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca
aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg
tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc
gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg
gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact
tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg
gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga
tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta
actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac
atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat
gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg
cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat
gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagtccctatcagtgatagagattgacatcc
ctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaa
gagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtc
gtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtc
cccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccg
gaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaag
acggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccat
gggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactg
aaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttaca
aaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcg
tcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata