BBa_K510041
type: ComponentDefinition
persistent identity: BBa K510041
display id: BBa_K510041
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm-RFP
created: 2011-10-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector.
owned by: myers
owned by: james
mutable description: This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The BBa_I13521 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm by EcoRI and PstI clonning. In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verify by analytic digestions.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_I13521
sequence annotation: BBa_K510000
sequence annotation: BBa_I13521
sequence: https://synbiohub.org/public/igem/BBa_K510041_sequence/1

BBa_K510000
type: Component
persistent identity: component2159805
display id: component2159805
version: 1
top level: BBa_K510041
definition: BBa_K510000
access: public

BBa_I13521
type: Component
persistent identity: component2159822
display id: component2159822
version: 1
top level: BBa_K510041
definition: BBa_I13521
access: public

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2159805
display id: annotation2159805
version: 1
top level: BBa_K510041
location: https://synbiohub.org/public/igem/BBa_K510041/annotation2159805/range2159805/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K510041/annotation2159805/range2159805/1
type: Range
persistent identity: range2159805
display id: range2159805
version: 1
top level: BBa_K510041
direction: direction/reverse
start: 1
end: 4388
orientation: inline

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2159822
display id: annotation2159822
version: 1
top level: BBa_K510041
location: https://synbiohub.org/public/igem/BBa_K510041/annotation2159822/range2159822/1
component: BBa_I13521

https://synbiohub.org/public/igem/BBa_K510041/annotation2159822/range2159822/1
type: Range
persistent identity: range2159822
display id: range2159822
version: 1
top level: BBa_K510041
direction: direction/reverse
start: 4397
end: 5319
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive on mRFP (positive control)
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
definition: BBa_B0010
access: public

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
top level: BBa_I13521
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
type: Range
persistent identity: range1532566
display id: range1532566
version: 1
top level: BBa_I13521
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
top level: BBa_I13521
direction: direction/forward
start: 81
end: 761
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
top level: BBa_I13521
direction: direction/forward
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
top level: BBa_I13521
direction: direction/forward
start: 883
end: 923
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restrictino site
sequence annotation: Gentamycin resistance
sequence annotation: NcoI restriction site
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
type: Range
persistent identity: range2137411
display id: range2137411
version: 1
top level: BBa_K510000
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
type: Range
persistent identity: range2137412
display id: range2137412
version: 1
top level: BBa_K510000
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
type: Range
persistent identity: range2137413
display id: range2137413
version: 1
top level: BBa_K510000
direction: direction/forward
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
type: Range
persistent identity: range2137414
display id: range2137414
version: 1
top level: BBa_K510000
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
top level: BBa_K510000
direction: direction/forward
start: 348
end: 360
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
type: Range
persistent identity: range2137416
display id: range2137416
version: 1
top level: BBa_K510000
direction: direction/forward
start: 966
end: 1826
orientation: inline

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
top level: BBa_K510000
direction: direction/forward
start: 1965
end: 2644
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3002
end: 3014
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3014
end: 3213
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3224
end: 3318
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3329
end: 3376
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3381
end: 3386
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3387
end: 3392
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
top level: BBa_K510000
direction: direction/reverse
start: 3393
end: 3926
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4184
end: 4189
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
type: Range
persistent identity: range2137440
display id: range2137440
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4196
end: 4243
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4249
end: 4268
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4326
end: 4359
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4367
end: 4387
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K510041_sequence/1
type: Sequence
persistent identity: BBa K510041 sequence
display id: BBa_K510041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13521_sequence/1
type: Sequence
persistent identity: BBa I13521 sequence
display id: BBa_I13521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510000_sequence/1
type: Sequence
persistent identity: BBa K510000 sequence
display id: BBa_K510000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z