LOCUS BBa_K510042 12033 bp DNA linear 26-Nov-2024 DEFINITION pUC18Sfi-miniTn7BB-Gm-Lux ACCESSION BBa_K510042 VERSION BBa_K510042.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix stem_loop 22..93 /label=E. coli his operon terminator primer_bind 157..176 /label=VR (BBa_G00101) primer binding site misc_feature 182..347 /label=Tn7-L end misc_feature 348..360 /label=SfiI restriction site CDS 966..1826 /label=Ampicillin resistance (bla) misc_feature 1965..2644 /label=ColEI (pUC18) Replication origin misc_feature 3002..3014 /label=SfiI restriction site misc_feature 3014..3213 /label=Tn7-R end misc_feature 3329..3376 /label=Flipase recombination sequence (FRT) CDS 3393..3926 /label=Gentamycin resistance misc_feature 4196..4243 /label=Flipase recombination sequence (FRT) misc_feature 4367..4387 /label=Prefix misc_feature 3224..3318 /label=double terminator (BBa_B0014) misc_feature 3381..3386 /label=SphI restriction site misc_feature 3387..3392 /label=NcoI restrictino site misc_feature 4184..4189 /label=NcoI restriction site misc_feature 4190..4195 /label=SphI restriction site primer_bind 4249..4268 /label=VF2 (G00100) primer binding site stem_loop 4326..4359 /label=Terminator misc_feature 1..4388 /label=BBa_K510000 misc_feature 4397..5475 /label=AraC promoter 5439..5510 /label=PC misc_feature 5476..5606 /label=pBAD CDS 5627..7056 /label=luxC CDS 8025..9089 /label=luxA CDS 9128..10108 /label=luxB CDS 10222..11319 /label=luxE CDS 11323..12033 /label=luxG RBS 5607..5626 /label=putative rbs region RBS 7060..7064 /label=putative rbs RBS 9115..9120 /label=putative rbs CDS 7071..7974 /label=luxD RBS 8012..8017 /label=putative rbs RBS 10171..10177 /label=putative rbs RBS 11311..11315 /label=putative rbs misc_feature 4397..12033 /label=BBa_K325909 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg 2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 3001 tggccgccta ggcctgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 3061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 3121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 3181 gcaaattgcc cgtcgtatta aagaggggcg tggggttcgc ttgtcacact ggctcacctt 3241 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat 3301 ccggcttttt tattatttgc tagatcttga agtacctatt ccgaagttcc tattctctaa 3361 aaagtatagg aacttcagag gcatgcccat ggttaggtgg cggtacttgg gtcgatatca 3421 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca 3481 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg 3541 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca 3601 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg 3661 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag 3721 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca 3781 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat 3841 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc 3901 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 3961 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata 4021 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga 4081 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt 4141 caattcgatc cattgctgtt gacaaaggga atcaggggat cttccatggg catgcgaagt 4201 acctattccg aagttcctat tctctaaaaa gtataggaac ttcagagctg ccacctgacg 4261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 4321 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 4381 ttctagagta ctagagttat gacaacttga cggctacatc attcactttt tcttcacaac 4441 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga 4501 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca 4561 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta 4621 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc 4681 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta 4741 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca 4801 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt 4861 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga 4921 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac 4981 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa 5041 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat 5101 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg 5161 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca 5221 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt 5281 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc 5341 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca 5401 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc 5461 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat 5521 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt 5581 ctccataccc gtttttttgg gctagcaggg aaaataatga ataaatgtat tccaatgata 5641 attaatggaa tgattcaaga ttttgataat tatgcatata aagaagttaa actaaataat 5701 gataatagag taaaattatc tgtcattact gaaagttcag tttcaaaaac attaaatatc 5761 aaagatagaa ttaatctaaa tttaaatcag attgtgaatt ttttatatac cgttggtcaa 5821 cgatggaaaa gtgaagaata taatcggcga cgaacctata tccgtgagtt aaaaacatat 5881 cttggttatt ctgatgaaat ggcaagatta gaagcgaatt ggattgcaat gttattgtgc 5941 tctaaaagtg ctttgtatga cattgttaat tatgatttgg gctctataca cgtattagat 6001 gaatggcttc cacgtggtga ttgctatgtt aaagcacaac cgaaaggtgt ttctgttcac 6061 ttgttagctg gtaatgttcc attatcagga gtgacatcta ttttgcgtgc tattttaaca 6121 aaaaatgagt gcattattaa aacttcgtct tcagatcctt ttactgcaaa cgctttagtt 6181 tccagtttta ttgatgttaa tgcagaccat ccaataacca aatcaatgtc tgttatgtat 6241 tggccgcatg atgaagatat gactctatct caaagaataa tgaatcatgc cgacgtggtt 6301 attgcttggg gtggagacga ggcgattaaa tgggcggtaa aatattcacc accgcatgtc 6361 gatattctga aatttggacc aaagaaaagc ttaagtatta ttgaagctcc taaagatata 6421 gaagccgcag caatgggggt tgctcatgat atttgtttct atgaccagca agcctgcttc 6481 tctactcaag acgtttatta tataggagat aatttacctt tatttttaaa tgaacttgaa 6541 aaacagctag atcgatacgc gaaaatttta ccaaaaggtt caaatagttt tgatgaaaaa 6601 gcggcgttta ctcttactga aaaagaaagt ctatttgctg gatatgaagt gagaaaggga 6661 gataagcaag cttggttaat agtcgtatca cctacaaata gctttggaaa tcaaccgcta 6721 tcacgaagtg tgtatgttca tcaagtatct gatattaaag agataattcc ttttgttaat 6781 aaaaatagaa cacaaactgt ttctatttat ccttgggaag cgtcattaaa atatcgagat 6841 aaattagcaa gaagtggagt tgaaagaatt gttgaatcag gcatgaataa tattttcaga 6901 gttggagggg ctcatgattc attatctcct ctccagtacc tagttaggtt tgtatcgcat 6961 gagagaccat ttaattatac gacaaaagat gttgcggttg aaatcgaaca aacacgttac 7021 ttagaggaag ataagttttt agtttttgtc ccatagttaa aggaaattat atgaaagatg 7081 aaagtgcttt ttttacgatt gatcacatca tcaagcttga taatggtcag tctatccgag 7141 tttgggaaac actccctaaa aagaacgtac cagagaaaaa acatacaata cttattgctt 7201 cgggttttgc tagaagaatg gatcattttg caggtcttgc tgagtattta tctactaacg 7261 gttttcatgt cattcgctac gattctttgc atcatgttgg attaagcagt ggatgtataa 7321 atgaatttac gatgtcgatt ggaaaaaata gcctgcttac agtcgtagat tggcttacag 7381 atcatggtgt cgaacgaata gggctgattg ctgctagttt gtcagcgaga atcgcctatg 7441 aggtagtaaa taaaattaaa ttatcatttt taattacggc cgtaggtgtc gttaatctta 7501 gagatacatt agaaaaagca ttggagtatg actatttgca attacctatt tcagagttac 7561 cagaagatct tgactttgaa ggtcataatt taggatcgga ggtctttgtt acagattgct 7621 ttaaacataa ttgggacacg ttagactcga cacttaatag tgttaaagga ttagcgattc 7681 catttattgc ttttactgca aacgatgata gttgggtaaa gcaaagtgaa gttatagagc 7741 tcattgatag cattgaatct agtaattgta agctctattc gctaattgga agttcacatg 7801 atcttgggga aaatttggtt gtattaagaa atttttatca atcagtgacg aaggcagcct 7861 tagcattaga tgatggttta ttggatttag agatagacat tattgaacct cgatttgagg 7921 acgttacaag tattactgtt aaggagcgta gattaaaaaa tgaaattgaa aatgaattat 7981 tagaattggc ttaaataaac agaatcacca aaaaggaata gagtatgaag tttggaaata 8041 tttgtttttc gtatcaacca ccaggtgaaa ctcataagca agtaatggat cgctttgttc 8101 ggcttggtat cgcctcagaa gaggtagggt ttgatacata ttggacctta gaacatcatt 8161 ttacagagtt tggtcttacg ggaaatttat ttgttgctgc ggctaacctg ttaggaagaa 8221 ctaaaacatt aaatgttggc actatggggg ttgttattcc gacagcacac ccagttcgac 8281 agttagaaga cgttttatta ttagatcaaa tgtcgaaagg tcgttttaat tttggaaccg 8341 ttcgagggct ataccataaa gattttcgag tatttggtgt tgatatggaa gagtctcgag 8401 caattactca aaatttctac cagatgataa tggaaagctt acagacagga accattagct 8461 ctgatagtga ttacattcaa tttcctaagg ttgatgtata tcccaaagtg tactcaaaaa 8521 atgtaccaac ctgtatgact gctgagtccg caagtacgac agaatggcta gcaatacaag 8581 ggctaccaat ggttcttagt tggattattg gtactaatga aaaaaaagca cagatggaac 8641 tctataatga aattgcgaca gaatatggtc atgatatatc taaaatagat cattgtatga 8701 cttatatttg ttctgttgat gatgatgcac aaaaggcgca agatgtttgt cgggagtttc 8761 tgaaaaattg gtatgactca tatgtaaatg cgaccaatat ctttaatgat agcaatcaaa 8821 ctcgtggtta tgattatcat aaaggtcaat ggcgtgattt tgttttacaa ggacatacaa 8881 acaccaatcg acgtgttgat tatagcaatg gtattaaccc tgtaggcact cctgagcagt 8941 gtattgaaat cattcaacgt gatattgatg caacgggtat tacaaacatt acatgcggat 9001 ttgaagctaa tggaactgaa gatgaaataa ttgcttccat gcgacgcttt atgacacaag 9061 tcgctccttt cttaaaagaa cctaaataaa ttacttattt gatactagag ataataagga 9121 acaagttatg aaatttggat tattttttct aaactttcag aaagatggaa taacatctga 9181 agaaacgttg gataatatgg taaagactgt cacgttaatt gattcaacta aatatcattt 9241 taatactgcc tttgttaatg aacatcactt ttcaaaaaat ggtattgttg gagcacctat 9301 taccgcagct ggttttttat tagggttaac aaataaatta catattggtt cattaaatca 9361 agtaattacc acccatcacc ctgtacgtgt agcagaagaa gccagtttat tagatcaaat 9421 gtcagaggga cgcttcattc ttggttttag tgactgcgaa agtgatttcg aaatggaatt 9481 ttttagacgt catatctcat caaggcaaca acaatttgaa gcatgctatg aaataattaa 9541 tgacgcatta actacaggtt attgtcatcc ccaaaacgac ttttatgatt ttccaaaggt 9601 ttcaattaat ccacactgtt acagtgagaa tggacctaag caatatgtat ccgctacatc 9661 aaaagaagtc gtcatgtggg cagcgaaaaa ggcactgcct ttaacattta agtgggagga 9721 taatttagaa accaaagaac gctatgcaat tctatataat aaaacagcac aacaatatgg 9781 tattgatatt tcggatgttg atcatcaatt aactgtaatt gcgaacttaa atgctgatag 9841 aagtacggct caagaagaag tgagagaata cttaaaagac tatatcactg aaacttaccc 9901 tcaaatggac agagatgaaa aaattaactg cattattgaa gagaatgcag ttgggtctca 9961 tgatgactat tatgaatcga caaaattagc agtggaaaaa acagggtcta aaaatatttt 10021 attatccttt gaatcaatgt ccgatattaa agatgtaaaa gatattattg atatgttgaa 10081 ccaaaaaatc gaaatgaatt taccataata aaattaaagg caatttctat attagattgc 10141 ctttttaaat ttctgttgat attaggtatt actggagagg gtatgactgt ccatactgaa 10201 tataaaagaa atcaaatcat tgctagttca gaaattgatg atcttatctt tatgacgaaa 10261 ccacaagagt ggtcatttga agagcaaaaa gaaatacggg ataaattagt tcgtgaggct 10321 ttttattttc actacaatag aaatgaagaa tatagaaatt attgtatcaa tcagcatgtg 10381 agtgataatt tacacactat tgatgaaata cccgtgtttc caacatctgt ttttaaatat 10441 aagaaattac atactgtcac agccgaggac attgaaaatt ggtatacaag tagtggaact 10501 cgtggagtaa aaagtcatat tgcacgtgat cgtcttagca ttgaacgctt gcttggttct 10561 gtcaacttcg gaatgaaata cgttggagat tggtttgagc atcaaatgga attgataaat 10621 ttaggaccag atagattcaa tacaaataat atttggttta aatatgtcat gagtttggtc 10681 gagttacttt atccgactga atttacagtt gataatgaca aaatagattt tgaaaaaaca 10741 gtaaaacatc tatttagaat taagaatagt aaaaaagaca tttgcttaat tgggccacca 10801 ttttttgtgt atcttttgtg ccaatatatg aaagaaaaca atattgaatt taaaggagga 10861 gatagagtac atattattac tggtggagga tggaaatcta atcagaatga ctctttagat 10921 cgtgctgatt ttaatcaatt attaatggat actttccaac tcgacaagat taatcaaatt 10981 agagatacct ttaatcaagt tgagcttaat acttgttttt ttgaagatga atttcaaaga 11041 aaacatgttc caccgtgggt atatgctcgg gctcttgatc ctgaaacctt gaaacccgta 11101 gcagatggtg agatcgggtt gttaagttat atggacgcct catcaactgc ttaccctgct 11161 tttattgtta ctgatgatat cggtattgta aaagaaatta gagaaccaga tccttaccca 11221 ggggtaactg ttgagattgt tcggcgctta aatacacgtg cgcaaaaagg atgcgcgctc 11281 tctatggcta atgtcataca aaagaatatc aaggattaag ttatgattgt tgatggtaga 11341 gtttcaaaaa tagtattagc gtcaataaga aataatatat ataaggtatt tattactgta 11401 aattcaccaa taaagttcat cgctggacaa tttgtaatgg tcacgattaa tgggaaaaaa 11461 tgcccttttt caattgcgaa ttgcccgaca aaaaattacg aaatagaatt gcatattggt 11521 agttcgaata gagactgctc attggatatt atcgaatatt ttgtcgatgc tcttgttgag 11581 gaagtcgcaa ttgagttaga tgctccccat ggaaacgctt ggttacggtc tgaaagtaat 11641 aacccattgc tattaattgc gggaggtaca ggtttatcat atataaatag cattctaaca 11701 aattgcttaa ataggaatat acctcaagat atttatcttt actggggagt aaaagacagt 11761 tctcttttgt atgaagatga agagttacta aacttatcac taaacaacaa aaactttcat 11821 tatattcctg ttattgaaga taaaagtgaa gaatggatag ggagaaaagg cactgttctt 11881 gatgctgtca tggaagattt tactgatctt acttattttg atatttatgt ttgtggaccc 11941 ttcatgatgg ctaaaacagc aaaagaaaaa ttaattgaag agaaaaaagc aaagtcagaa 12001 cagatgtttg ccgatgcttt tgcatacgta taa //