##gff-version 3
##sequence-region BBa_K510042 1 12033
BBa_K510042	.	engineered_region	4397	12033	.	+	0	ID=BBa_K325909;Name=BBa_K325909
BBa_K510042	.	ribosome_entry_site	10171	10177	.	+	0	ID=annotation2112119;Name=putative rbs;Parent=BBa_K325909
BBa_K510042	.	CDS	10222	11319	.	+	0	ID=annotation2112058;Name=luxE;Parent=BBa_K325909
BBa_K510042	.	ribosome_entry_site	11311	11315	.	+	0	ID=annotation2112122;Name=putative rbs;Parent=BBa_K325909
BBa_K510042	.	CDS	9128	10108	.	+	0	ID=annotation2112057;Name=luxB;Parent=BBa_K325909
BBa_K510042	.	promoter	5439	5510	.	+	0	ID=annotation2112036;Name=PC;Parent=BBa_K325909
BBa_K510042	.	ribosome_entry_site	8012	8017	.	+	0	ID=annotation2112117;Name=putative rbs;Parent=BBa_K325909
BBa_K510042	.	CDS	8025	9089	.	+	0	ID=annotation2112056;Name=luxA;Parent=BBa_K325909
BBa_K510042	.	CDS	7071	7974	.	+	0	ID=annotation2112076;Name=luxD;Parent=BBa_K325909
BBa_K510042	.	CDS	11323	12033	.	+	0	ID=annotation2112059;Name=luxG;Parent=BBa_K325909
BBa_K510042	.	sequence_feature	4397	5475	.	+	0	ID=annotation2111897;Name=AraC;Parent=BBa_K325909
BBa_K510042	.	sequence_feature	5476	5606	.	+	0	ID=annotation2112038;Name=pBAD;Parent=BBa_K325909
BBa_K510042	.	CDS	5627	7056	.	+	0	ID=annotation2112039;Name=luxC;Parent=BBa_K325909
BBa_K510042	.	ribosome_entry_site	9115	9120	.	+	0	ID=annotation2112062;Name=putative rbs;Parent=BBa_K325909
BBa_K510042	.	ribosome_entry_site	7060	7064	.	+	0	ID=annotation2112061;Name=putative rbs;Parent=BBa_K325909
BBa_K510042	.	ribosome_entry_site	5607	5626	.	+	0	ID=annotation2112060;Name=putative rbs region;Parent=BBa_K325909
BBa_K510042	.	plasmid	1	4388	.	+	0	ID=BBa_K510000;Name=BBa_K510000
BBa_K510042	.	sequence_feature	3387	3392	.	+	0	ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	3329	3376	.	+	0	ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510042	.	stem_loop	4326	4359	.	+	0	ID=annotation2137449;Name=Terminator;Parent=BBa_K510000
BBa_K510042	.	primer_binding_site	157	176	.	+	0	ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000
BBa_K510042	.	engineered_region	4367	4387	.	+	0	ID=annotation2137423;Name=Prefix;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	3014	3213	.	+	0	ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000
BBa_K510042	.	engineered_region	2	21	.	+	0	ID=annotation2137411;Name=Suffix;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	3381	3386	.	+	0	ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	4190	4195	.	+	0	ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	3002	3014	.	+	0	ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510042	.	stem_loop	22	93	.	+	0	ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	4196	4243	.	+	0	ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510042	.	CDS	966	1826	.	+	0	ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000
BBa_K510042	.	engineered_region	3224	3318	.	+	0	ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000
BBa_K510042	.	sequence_feature	182	347	.	+	0	ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	348	360	.	+	0	ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	1965	2644	.	+	0	ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000
BBa_K510042	.	sequence_feature	4184	4189	.	+	0	ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000
BBa_K510042	.	primer_binding_site	4249	4268	.	+	0	ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000
BBa_K510042	.	CDS	3393	3926	.	+	0	ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000
>BBa_K510042
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt
gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac
gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc
cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg
tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct
atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc
cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat
attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt
ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg
gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc
tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt
ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca
tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac
gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt
agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta
ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac
caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct
gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga
gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt
tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat
taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa
gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg
gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac
tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca
aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg
tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc
gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg
gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact
tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg
gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga
tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta
actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac
atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat
gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg
cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat
gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagttatgacaacttgacggctacatcattc
actttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagtt
gatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgat
acgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaag
caaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgta
cccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttat
cgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctgg
tgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcg
gacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaa
cagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattg
agaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccg
ccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgcc
attcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacc
aaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtg
tctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccat
aagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcagg
gaaaataatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagtt
aaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagata
gaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcg
gcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggatt
gcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaat
ggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccatt
atcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatcctttt
actgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtatt
ggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacga
ggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagtatt
attgaagctcctaaagatatagaagccgcagcaatgggggttgctcatgatatttgtttctatgaccagcaagcctgct
tctctactcaagacgtttattatataggagataatttacctttatttttaaatgaacttgaaaaacagctagatcgata
cgcgaaaattttaccaaaaggttcaaatagttttgatgaaaaagcggcgtttactcttactgaaaaagaaagtctattt
gctggatatgaagtgagaaagggagataagcaagcttggttaatagtcgtatcacctacaaatagctttggaaatcaac
cgctatcacgaagtgtgtatgttcatcaagtatctgatattaaagagataattccttttgttaataaaaatagaacaca
aactgtttctatttatccttgggaagcgtcattaaaatatcgagataaattagcaagaagtggagttgaaagaattgtt
gaatcaggcatgaataatattttcagagttggaggggctcatgattcattatctcctctccagtacctagttaggtttg
tatcgcatgagagaccatttaattatacgacaaaagatgttgcggttgaaatcgaacaaacacgttacttagaggaaga
taagtttttagtttttgtcccatagttaaaggaaattatatgaaagatgaaagtgctttttttacgattgatcacatca
tcaagcttgataatggtcagtctatccgagtttgggaaacactccctaaaaagaacgtaccagagaaaaaacatacaat
acttattgcttcgggttttgctagaagaatggatcattttgcaggtcttgctgagtatttatctactaacggttttcat
gtcattcgctacgattctttgcatcatgttggattaagcagtggatgtataaatgaatttacgatgtcgattggaaaaa
atagcctgcttacagtcgtagattggcttacagatcatggtgtcgaacgaatagggctgattgctgctagtttgtcagc
gagaatcgcctatgaggtagtaaataaaattaaattatcatttttaattacggccgtaggtgtcgttaatcttagagat
acattagaaaaagcattggagtatgactatttgcaattacctatttcagagttaccagaagatcttgactttgaaggtc
ataatttaggatcggaggtctttgttacagattgctttaaacataattgggacacgttagactcgacacttaatagtgt
taaaggattagcgattccatttattgcttttactgcaaacgatgatagttgggtaaagcaaagtgaagttatagagctc
attgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttg
tattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacat
tattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaatta
ttagaattggcttaaataaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgtttttcgtatcaac
caccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggtttgatac
atattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacctgttagga
agaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaagacgttt
tattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttcgagtatt
tggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagacaggaacc
attagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgtaccaacct
gtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttggattattgg
tactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaaaatagat
cattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctgaaaaatt
ggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcataaaggtca
atggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccctgtaggc
actcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcggatttgaag
ctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaagaacctaa
ataaattacttatttgatactagagataataaggaacaagttatgaaatttggattattttttctaaactttcagaaag
atggaataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattttaa
tactgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggttttttatta
gggttaacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaagaag
ccagtttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatggaatt
ttttagacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactacaggt
tattgtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggaccta
agcaatatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtggga
ggataatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcggat
gttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatacttaa
aagactatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagttgg
gtctcatgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatcctttgaa
tcaatgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataataaa
attaaaggcaatttctatattagattgcctttttaaatttctgttgatattaggtattactggagagggtatgactgtc
catactgaatataaaagaaatcaaatcattgctagttcagaaattgatgatcttatctttatgacgaaaccacaagagt
ggtcatttgaagagcaaaaagaaatacgggataaattagttcgtgaggctttttattttcactacaatagaaatgaaga
atatagaaattattgtatcaatcagcatgtgagtgataatttacacactattgatgaaatacccgtgtttccaacatct
gtttttaaatataagaaattacatactgtcacagccgaggacattgaaaattggtatacaagtagtggaactcgtggag
taaaaagtcatattgcacgtgatcgtcttagcattgaacgcttgcttggttctgtcaacttcggaatgaaatacgttgg
agattggtttgagcatcaaatggaattgataaatttaggaccagatagattcaatacaaataatatttggtttaaatat
gtcatgagtttggtcgagttactttatccgactgaatttacagttgataatgacaaaatagattttgaaaaaacagtaa
aacatctatttagaattaagaatagtaaaaaagacatttgcttaattgggccaccattttttgtgtatcttttgtgcca
atatatgaaagaaaacaatattgaatttaaaggaggagatagagtacatattattactggtggaggatggaaatctaat
cagaatgactctttagatcgtgctgattttaatcaattattaatggatactttccaactcgacaagattaatcaaatta
gagatacctttaatcaagttgagcttaatacttgtttttttgaagatgaatttcaaagaaaacatgttccaccgtgggt
atatgctcgggctcttgatcctgaaaccttgaaacccgtagcagatggtgagatcgggttgttaagttatatggacgcc
tcatcaactgcttaccctgcttttattgttactgatgatatcggtattgtaaaagaaattagagaaccagatccttacc
caggggtaactgttgagattgttcggcgcttaaatacacgtgcgcaaaaaggatgcgcgctctctatggctaatgtcat
acaaaagaatatcaaggattaagttatgattgttgatggtagagtttcaaaaatagtattagcgtcaataagaaataat
atatataaggtatttattactgtaaattcaccaataaagttcatcgctggacaatttgtaatggtcacgattaatggga
aaaaatgccctttttcaattgcgaattgcccgacaaaaaattacgaaatagaattgcatattggtagttcgaatagaga
ctgctcattggatattatcgaatattttgtcgatgctcttgttgaggaagtcgcaattgagttagatgctccccatgga
aacgcttggttacggtctgaaagtaataacccattgctattaattgcgggaggtacaggtttatcatatataaatagca
ttctaacaaattgcttaaataggaatatacctcaagatatttatctttactggggagtaaaagacagttctcttttgta
tgaagatgaagagttactaaacttatcactaaacaacaaaaactttcattatattcctgttattgaagataaaagtgaa
gaatggatagggagaaaaggcactgttcttgatgctgtcatggaagattttactgatcttacttattttgatatttatg
tttgtggacccttcatgatggctaaaacagcaaaagaaaaattaattgaagagaaaaaagcaaagtcagaacagatgtt
tgccgatgcttttgcatacgtataa